Anti-CD44 mAb remodels biological behaviors of spheroid cells with stemness from human ovarian cancer cell line SKOV-3

There is accumulating evidence that cancer stem cells (CSCs) play an important role in tumor progression. Novel strategies targeting CSCs have been widely researched. In the present study, we explored whether such CSCs existed in human ovarian cancer (OVCA) cell line and whether anti-CD44 antibody had effects on such subpopulation. We isolated and identified spheroid cells from SKOV-3. Then we used A3D8, an anti-CD44 mAb to treat spheroid cells with so-called "stemness". Effects of A3D8 on spheroid cells’ biological behaviors were examined. Our findings showed that there was a small subpopulation that had so-called "stemness" in SKOV-3 cell line. Against spheroid cells, A3D8 can (1) inhibit cell proliferation; (2) change cell cycle distribution and expression of p21, CDK2 and cyclinA; (3) enhance cisplatin (DDP)-induced apoptosis; (4) promote cell differentiation; (5) inhibit clone formation efficiency; (6) reduce invasive efficacy; (7) inhibit tumorigenicity. Thus, to sum up points which we have just showed, spheroid cells isolated from SKOV-3 can be used as an appropriate in vitro model for relevant study of human ovarian CSCs. And our results reasoned that anti-CD44 therapy may become a potential promising strategy for OVCA treatment.     

Discussion on the Construction Technology and Application of Civil Engineering Measurement

笔者结合自己多年的工作经验,阐述了建筑工程测量技术的相关问题及其应用.     

吸收剂量和增容剂对CR/PAANa遇水膨胀橡胶性能的影响

采用辐射硫化技术制备了氯丁/聚丙烯酸钠遇水膨胀橡胶,考察了在一定辐射剂量时增容剂对膨胀橡胶力学性能、膨胀性能和质量损失率的影响,以及辐射剂量对质量损失率的影响.结果表明,增容剂有利于提高力学和膨胀性能,降低质量损失率,辐射剂量提高有利于质量损失率的降低.     

Juvenile hominoid cranium from the terminal Miocene of Yunnan, China

Fossil apes are known from several late Miocene localities in Yunnan Province, southwestern China, principally from Shihuiba (Lufeng) and the Yuanmou Basin, and represent three species of Lufengpithecus. They mostly comprise large samples of isolated teeth, but there are also several partial or complete adult crania from Shihuiba and a single juvenile cranium from Yuanmou. Here we describe a new, relatively complete and largely undistorted juvenile cranium from the terminal Miocene locality of Shuitangba, also in Yunnan. It is only the second ape juvenile cranium recovered from the Miocene of Eurasia and it is provisionally assigned to the species present at Shihuiba, Lufengpithecus lufengensis. Lufengpithecus has most often been linked to the extant orangutan, Pongo pygmaeus, but recent studies of the crania from Shihuiba and Yuanmou have demonstrated that this is unlikely. The new cranium reinforces the view that Lufengpithecus represents a distinct, late surviving lineage of large apes in the late Miocene of East Asia that does not appear to be closely affiliated with any extant ape lineage. It substantially increases knowledge of cranial morphology in Lufengpithecus and demonstrates that species of this genus represent a morphologically diverse radiation of apes, which is consistent with the dynamic tectonic and biotic milieu of southwestern China in the late Miocene.     

Pascal定理的投影证明

给出了利用中心投影证明 Pascal 定理的过程,并用较为初等的方法,导出了一些结论.     

一种非线性竞争性Hebbian算法

与主量分析密切相关的Ｈｅｂｂｉａｎ算法大都局限于线性模型精确输入恢复前提，本文考察了一类非线性的竞争性Ｈｅｂｂｉａｎ学习，提出了一个确切的基于纯竞争机制的优化算法，仿真实验表明，该算法较之于现有的算法更为快速、有效。     

频率步进高距离分辨技术在圆锥扫体制中的应用

对频率步进高距离分辨技术在圆锥扫描栖制雷达中的应用做了探讨，分析了在锥扫描体制下利用频率步进高距离分辨技术获取目标的距离回波而据以识别目标的方法、目标识别和获取角误差信号的关系。     

盐生杜氏藻碱性蛋白的研究

以一种单细胞的盐生杜氏藻为实验材料，利用化学和机械的方法破碎细胞，然后梯度分离细胞核，用盐酸抽提细胞核蛋白质，并与小牛胸腺组蛋白相比较。用ＳＤＳ－ＰＡＧＥ分析测定所得的碱性蛋白发现盐生杜氏藻核中有三条碱性蛋白带，两条蛋白带与Ｈ３与Ｈ４相对应，另一条分子量很小。     

国产格栅加筋沥青混凝土抗车辙能力的研究

国产格栅加筋沥青混凝土抗车辙能力的研究杨军，邓学钧（东南大学交通学院，南京２１００１８）自１９８４年３月在伦敦举办的聚合物格栅加筋专题国际研讨会以来，各国道路专家学者通过多年的研究和实践，肯定了采用ＴＥＮＳＥＲ格栅、玻璃纤维格栅，对减少沥青路面车辙，...     

连续配筋混凝土路面端部锚固原理研究

首先分析了连续配筋路面端部移动计算原理，提出了端部锚固应力计算的迭代法，计算结果表明混凝土的弹性模量，线胀系数和温度变化量是影响锚固应力的主要因素，最后提出了合理的端部固应力计算方法。