N-terminal of L protein of vesicular stomatitis virus contains a new signal sequence

The L protein (241kD) of vesicular stomatitis virus (VSV) is the most important snbnnit of the replication complex. The existence of specific localization signal in the L protein was investigated by making recombinant constructs expressing truncated mutants of the L protein fused to green fluorescent protein (GFP) in transient transfection assays. The chimeric genes encoding varied N-terminal of L and GFP gene were put under the control of T7 promoter or CMV promoter. The fusion proteins were transiently expressed in BHK-21, COS-7, CHO or Hep G2 cells. When more than 120 residues were deleted or only 96 residues were kept on the N-terminal, the fusion proteins were shown to be distributed throughout the cells, cytoplasm and nucleus under the confocal microscope. However, other chimeric proteins with 120 or more amino acids were dotted and distributed in the perinuclear regions. And the fusion protein with 96—120 aa has the similar distribution. A thirteen-residue peptide QGYSFLHEVDKEA (108—120) was identified as localization signal, whose function would be absolutely distributed with the deficiency of D or V. Our results show that there is an independent localizing signal in N-terminal domain of L protein of VSV and this functional signal is conserved in different cell lines.     

Contributions of different land cover types in Otindag Sandy Land and Bashang area of Hebei Province to the material source of sand stormy weather in Beijing

Different land cover types in Otindag Sandy Land and Bashang area of Hebei Province are linked to a material source of sand stormy weather in Beijing based on results of field vegetation and soil survey and laboratory works. Results of grain size analysis show that dust release potential in per unit area of moving sandy land is small,while lowland meadow and meadow steppe in stony hills have high potential of releasing dusts in per unit area during sand stormy weather occurrence. Further considering the effects of vegetation, it is inferred that the moving sandy land served as a material source of past dust storm and the possibility of dust release in per unit area is low in current time.Typical steppe in stony hills is undergoing desertification and its dust release possibility in per unit area is high. Farmland has strong potential of release dusts when they are ploughed in spring, but a large amount of therophytes grow and thus prevent dusts from release when cultivation was terminated.Potentials of dust release in per unit area in fixed sand dunes,stony mountain meadow steppe and low land meadow are lowdue to high cover of perennials. Sand dune reactivation and desiccation of lakes and lowlands under estimated future climatic change will make them serve as a future material source of sand stormy weather.     

基于GIS和VR的矿图数字化

从矿山系统工程角度，分析了矿图以及使用CAD技术实现其数字化工作的意义，探讨了矿图数字化建立在地理信息系统（GIS）和虚拟现实技术（VR）基础上的相关措施，讨论了进行该项工作需要使用的软件，目前基于GIS和VR的矿图数字化工作，已经取得了进展，进一步的研究有理论和应用方面的重要价值。     

论对环境因素全方位的控制

从资源，固体废弃物，废水、废气，绿树、绿地等方面阐述了只有对环境因素进行全方位控制，才能实现最佳物业管理服务效用，创造优美、舒适、温馨的社区环境。     

AIP吸收器传质特性中试装置及KLa测试方法研究

基于不依赖空气动力装置（AIP）排出CO2气体旋转床吸收器传质特性研究要求，选择S-195型柴油机为AIP排出气体发生器，优化设计了气、水流程，建立了波纹碟片填料旋转床吸收器的传质特性中间试验装置（简称中试装置），该装置具有多参数调节和测试功能。针对在较高气体压力下，AIP排出气体旋转床传质试验无法直接测算其体积传质系数（Kla），提出了一种间接测算Kla实验值的方法。实验结果表明，用间接测算方法求取的Kla实验值与直接测算值相对误差小于10％。     

时滞分布参数系统变结构控制器的设计

研究了一类多时滞抛物型分布参数系统的变结构控制问题．通过非奇线性变换建立了系统的滑动模运动方程，设计了系统的无记忆功能的变结构控制器，给出了运动轨线到达滑动模态区的时间的估计．     

热通道玻璃幕墙的热工计算

简述了热通道的节能原理，推导出了热通道玻璃幕墙的热工计算模型，确定了模型中各方程的系数，介绍了计算及测量结果。该模型可用来计算内外侧玻璃的壁面温度、热通道内空气的温度和流速，以及室内的得热速率等，为相关工程计算提供参考。     

H_0~1[a,b]中的最佳数值积分公式

本文利用H10[a，b]中样条插值算子理论，讨论了H10[a，b]中的最佳逼近泛函，并给出最佳数值积分公式。     

结构系统疲劳可靠性分析方法及其应用

研究了结构系统疲劳可靠性分析方法，建立了结构系统疲劳可靠性分析模型，提出了求取结构系统疲劳主要失效模式的方法，最后，以一海上平台为工程实际算例验证了文中算法的可行性及有效性。     

带堆焊层钢管焊接接头耐蚀性的电化学评定

采用电化学测试技术评价了低碳钢管管端内壁不锈钢焊条堆焊后再用相同焊条进行打底焊所得焊接接头（特别是焊接热影响区）的耐蚀性，选用两种高合金焊条M2、M6，并对堆焊金属进行热模拟试验，测定了模拟热影响区不同部位在3％NaCl介质中的阳极极化行为．结果表明，按新工艺焊接的接头代替普通碳钢焊接接头可有效地提高管道接头的使用寿命．两种焊条中，含Nb的M2耐蚀性比M6好，铌能够有效提高接头的耐均匀腐蚀、晶间腐蚀和点蚀能力，热影响区600～1000℃是高合金接头耐蚀性最薄弱的区域，建议采用超低碳含铌高合金焊条，以解决热影响区的腐蚀问题．