Natural engineering principles of electron tunnelling in biological oxidation-reduction

We have surveyed proteins with known atomic structure whose function involves electron transfer; in these, electrons can travel up to 14 A between redox centres through the protein medium. Transfer over longer distances always involves a chain of cofactors. This redox centre proximity alone is sufficient to allow tunnelling of electrons at rates far faster than the substrate redox reactions it supports. Consequently, there has been no necessity for proteins to evolve optimized routes between redox centres. Instead, simple geometry enables rapid tunnelling to high-energy intermediate states. This greatly simplifies any analysis of redox protein mechanisms and challenges the need to postulate mechanisms of superexchange through redox centres or the maintenance of charge neutrality when investigating electron-transfer reactions. Such tunnelling also allows sequential electron transfer in catalytic sites to surmount radical transition states without involving the movement of hydride ions, as is generally assumed. The 14 A or less spacing of redox centres provides highly robust engineering for electron transfer, and may reflect selection against designs that have proved more vulnerable to mutations during the course of evolution.     

The p66shc adaptor protein controls oxidative stress response and life span in mammals

Gene mutations in invertebrates have been identified that extend life span and enhance resistance to environmental stresses such as ultraviolet light or reactive oxygen species. In mammals, the mechanisms that regulate stress response are poorly understood and no genes are known to increase individual life span. Here we report that targeted mutation of the mouse p66shc gene induces stress resistance and prolongs life span. p66shc is a splice variant of p52shc/p46shc (ref. 2), a cytoplasmic signal transducer involved in the transmission of mitogenic signals from activated receptors to Ras. We show that: (1) p66shc is serine phosphorylated upon treatment with hydrogen peroxide (H2O2) or irradiation with ultraviolet light; (2) ablation of p66shc enhances cellular resistance to apoptosis induced by H2O2 or ultraviolet light; (3) a serine-phosphorylation defective mutant of p66shc cannot restore the normal stress response in p66shc-/- cells; (4) the p53 and p21 stress response is impaired in p66shc-/- cells; (5) p66shc-/- mice have increased resistance to paraquat and a 30% increase in life span. We propose that p66shc is part of a signal transduction pathway that regulates stress apoptotic responses and life span in mammals.     

模式识别与计算机视觉手册第三版

本书的第一、二、三版分别于1993、1999和2005年出版。书中全面提供了过去20年中在模式识别与计算机视觉领域中的进展和成就，作者都是这个领域的第一流专家，其中的两位Thomas　Huang和Jake　Aggarwal是权威的K．S．Fu奖金获得者，该项奖金是由国际模式识别协会（IAPR）授予。     

1-(substituted)benzyl-5-aminoimidazole-4-carboxamides are potent orally active inhibitors of Trypanosoma cruzi in mice

1-(Substituted)benzyl-5-aminoimidazole-4-carboxamides are potent orally active inhibitors of Trypanosoma cruzi infections in mice. The most active compounds are the 1-(4-chlorobenzyl)- and 1-(3,4-dichlorobenzyl)-analogs (L-153,094 [2] and L-153,153 [4], resp.) which are approximately 7-fold more potent upon oral administration than nifurtimox (Lampit) in suppressing parasite levels in the blood of mice with acute Trypanosoma cruzi infections.     

测井资料分析系统接口电路设计

从一种超声模拟测井记录的井下信号、深度信号送微机处理的实际要求出发，分析井下信号数据采集的工作原理，阐述了与１２位Ａ／Ｄ转换器实时配套工作的双存储体工作时序。通过对深度信号的解调，提出了一种解决４ＦＳＫ解调的有效方法。     

BP网络的抗干扰性研究及误差分析

从ＢＰ网络对信息存储的分布式特点出发，分析了连接权系数及输入变化对输出的影响，探讨了ＢＰ网络的容错性及抗干扰特性，得出了一些有益的结论。     

任意波形合成系统硬件设计

提出并分析了任意波形合成系统（ＡＷＳ）──一种高度智能化的计算机仪器的几种典型结构及其特点，着重讨论了ＰＣ总线式任意波形合成系统的系统组成及各部分硬件电路设计的关键技术     

风险投资公司经营能力的模糊评价方法

针对风险投资公司的经营能力问题 ,依据风险投资的运作机制和风险公司经营活动的特征 ,首先建立了一套包括业绩与经验、管理能力、人力资源和资产状况等 4个方面的评价指标体系 ,共涉及 1 0个指标 .然后在此基础上 ,针对建立的指标体系中既有主观指标又有客观指标的特点 ,给出了一种模糊多指标评价方法 .最后通过一个实例计算说明了给出方法的应用 .     

Wireless Location Determination for Mobile Objects Based on GSM in Intelligent Transportation Systems

1 .INTRODUCTIONTheabilityofpositioningmobileobjects (MOs) ,in cludingvehiclesandtravelersholdingmobiletermi nals,isafundamentalfunctionforsomeITSsubsys tems,suchasemergencyrescue,securitysystem ,au tomaticnavigationsystem ,andtrafficinducementsystem[1 ] .Atpresent,therearethreedominatingtypes[2 ]ofwirelesspositioningtechnologies,i.e.positioningbasedonsatellites,positioningbasedondedicatedcommunicationsystemsandpositioningbasedonpub liclandwirelesstelecommunicationsystems (PLMTS) ,respe…     

Monoclonal antibodies to L-asparaginase

Five hybridomas secreting monoclonal antibody to E. coli L-asparaginase were isolated. These monoclonal antibodies were classified into 3 different subclasses; Ig G1 (1 clone), Ig G2 (2 clones) and Ig G3 (2 clones). One of them possessed anti-L-asparaginase neutralizing activity. Four antibodies examined demonstrated a linear Langmuir binding plot and binding affinities, with equilibrium dissociation constant (Kd) ranging between 2.5 X 10(-9) M and 6.3 X 10(-10) M. The monoclonal antibodies should be useful probes for investigation of the enzyme activity.