A rapid and accurate 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric assay for quantification of bacteriocins with nisin as an example

The objective of this study is to propose a more accurate and faster MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay (MCA) for quantitative measurement of polypeptide bacteriocins in solutions with nisin as an example. After an initial incubation of nisin and indicator bacterium Micrococcus luteus NCIB 8166 in tubes, MTT was added for another incubation period. After that, nisin was quantified by estimating the number of viable bacteria based on measuring the amount of purple formazan produced by cleavage of yellow tetrazolium salt MTT. Then MCA was compared to a standard agar diffusion assay (ADA). The results suggested a high correlation coefficient (r ²=0.975±0.004) between optical density (OD) and the inhibitory effect of nisin on a bacterial strain Micrococcus luteus NCIB 8166 at a range of 0.125~32 IU/ml. The MCA described in this study was very quick. Quantification of nisin took only 7~8 h and the detection limit was at the level of 0.125 IU/ml when compared to 12 IU/ml and 24~28 h for ADA. The MCA provides an accurate and rapid method for quantification of nisin in solutions and is expected to be used for quantification of other antimicrobial substances.     

临近空间单粒子效应的数值模型和电路模拟

针对临近空间单粒子效应进行了数值模型仿真和特征尺寸为0.1 μm的反相器电路的脉冲注入模拟研究。数值仿真结果表明器件临界电荷随着工作电压的降低而减小,敏感横截面随着临界电荷的降低而逐渐增大。临近空间微电子器件的单粒子翻转概率随敏感横截面增大而上升,但其又随临近空间高度的增加而下降。此外,利用SPICE软件脉冲注入模拟观察到了反相器电路的单粒子翻转现象。所得结论有助于深入研究临近空间的单粒子效应并为器件抗辐射加固提供了理论依据。     

信号与系统中的matlab程序

在现行的理工科教学中,matlab的应用已经成为一个重要的内容。尤其在电子类的核心专业课信号与系统中,matlab的作用更加值得教师关注。文章就信号与系统这一课程中牵涉到的一些典型matlab程序,进行了注解和说明。试图在信号与系统和matlab的应用两方面找到了一个更为恰当的结合点,使学生和教师在专业知识和应用性工具方面,得到一个更好的处理方式。     

基于循环经济模式的交通运输可持续发展研究

文章阐明了循环经济的基本概念和基本理论，在分析我国交通运输业可持续发展面临的诸多问题后，结合循环经济模式对我国交通运输可持续发展战略进行了研究。     

高校网络教育学习支持服务系统的研究与改进

通过对四川大学网络教育学院学习支持服务体系现状的调研,提出了改进学习支持服务体系建设的相关建议。     

Multiple MIPS 4Kc cores based interrupt controller design and its implementation on HDTV SoC platform

A multiple MIPS 4Kc processor cores based interrupt processing system is introduced. The interrupt controller plays a key role in the high definition television （HDTV） system-on-a-chip （SoC） platform, especially when it is a multiple processor system. Based on a general introduction to the whole HDTV SoC platform, a layered interrupt controller and its implementation are discussed in detail. The proposed scheme was implemented in our FPGA verification board. The results indicate that our scheme is reliable and efficient. Meanwhile, as a functional intellectual property （IP）, the interrupt controller has reusability and expandability with the layered structure.     

寻找精神家园的“歌手”——《浮士德》探幽

歌德的《浮士德》是“一种生活的发现艺术”。通过阅读作品，我们就会发现无论是“浮士德”形象还是“浮士德精神”，其最终的指向都是人的心灵世界，是人类追求和寻找精神家园的一种艺术化表达，浮士德的一生是寻找精神家园的一次审美体验。     

基于时态关系模型的儿童保健系统的设计研究

根据当前时态数据模型的理论,为儿童保健管理信息系统建立了一个可行的时态关系模型,并对系统中的查询做了简单的描述,最后分析了这种建模方法的优缺点.     

果胶溶液粘度的研究

本文研究了浓度、温度、酸度、Ｃａ２＋、糖等因素对果胶溶液粘度的影响，在酸度为０．９ｇＨＣｌ／１０Ｏｍｌ下来胶容液的粘度最低、而Ｃａ２＋，糖在研究的范围内对果胶溶液粘度的影响不大。     

态叠加原理及其在结构化学上的应用

本文对态叠加原理的物理实质进行了讨论。又重点地介绍了态叠加原理在结构化学上三个方面的应用。