逆向物流网络结构研究

文章介绍了逆向物流概念,分析了发展逆向物流的必要性和紧迫性,从逆向物流的结构特征出发,以不同的角度对逆向物流系统做了分类研究,然后从逆向物流的网络着手,分析了逆向物流网络的构建环境、网络特征和网络类型。     

线性代数：研究生必备，第二版

本书是一本高级线性代数教材。线性代数是充满活力的经典数学分支之一，无论在纯粹或应用数学、物理和工程、计算机科学及社会科学中都有广泛应用。由于现在流行的线性代数教材在理论深度和取材范围上的局限，一些专业的研究生在对理论证明和数值算法的理解上都存在某些不足。     

关于可对称化矩阵的Weyl-Лидский型定理

建立了可对称化矩阵情形下的型定理和与近似不变子空间相关的特征值扰动分析.拓广了W Kahan的相应结果.     

几种重要工业沸石概况

介绍了NaA、NaY和ZSM-5等重要沸石的工业合成.讨论了合成中的化学、技术及经济等因素.此外,还描述了SiCl_4脱铝和沸石的成型等过程.     

鲁米诺—过氧化氢—奎宁化学发光体系测定微量奎宁

本文报导了鲁米诺—过氧化氢—奎宁化学发光体系,建立了测定微量奎宁的最佳条件。对6.6×10~(-6)mol/L奎宁测定的变异系数为1%。该法可用于测定药物中的奎宁,结果良好。     

Transgenesis in fish

Gene transfer into fish embryo is being performed in several species (trout, salmon, carps, tilapia, medaka, goldfish, zebrafish, loach, catfish, etc.). In most cases, pronuclei are not visible and microinjection must be done into the cytoplasm of early embryos. Several million copies of the gene are generally injected. In medaka, transgenesis was attempted by injection of the foreign gene into the nucleus of oocyte. Several reports indicate that the injected DNA was rapidly replicated in the early phase of embryo development, regardless of the origin and the sequence of the foreign DNA. The survival of the injected embryos was reasonably good and a large number reached maturity. The proportion of transgenic animals ranged from 1 to 50% or more, according to species and to experimentators. The reasons for this discrepancy have not been elucidated. In all species, the transgenic animals were mosaic. The copy number of the foreign DNA was different in the various tissues of an animal and a proportion lower than 50% of F1 offsprings received the gene from their parents. This suggests that the foreign DNA was integrated into the fish genome at the two cells stage or later. An examination of the integrated DNA in different cell types of an animal revealed that integration occurred mainly during early development. The transgene was found essentially unrearranged in the fish genome of the founders and offsprings. The transgenes were therefore stably transmitted to progeny in a Mendelian fashion. Southern blot analysis revealed the presence of possible junction fragments and also of minor bands which may result from a rearrangement of the injected DNA. In all species, the integrated DNA appeared mainly as random end-to-end concatemers. In adult trout blood cells, a small proportion of the foreign DNA was maintained in the form of non-integrated concatemers, as judged by the existence of end fragments. The transgenes were generally only poorly expressed. The majority of the injected gene constructs contained essentially mammalian or higher vertebrates sequences. The comparison of the expression efficiency of these constructs in transfected fish and mammalian cells indicates that some of the mammalian DNA sequences are most efficiently understood by the fish cell machinery. Chloramphenicol acetyl transferase gene under the control of promoters from Rous sarcoma virus, and human cytomegalovirus, was expressed in several tissues of transgenic fish. Chicken delta-crystallin gene was expressed in several tissues of transgenic fish.(ABSTRACT TRUNCATED AT 400 WORDS)