全文获取类型
收费全文 | 31084篇 |
免费 | 88篇 |
国内免费 | 97篇 |
专业分类
系统科学 | 339篇 |
丛书文集 | 543篇 |
教育与普及 | 64篇 |
理论与方法论 | 116篇 |
现状及发展 | 12945篇 |
研究方法 | 1317篇 |
综合类 | 15448篇 |
自然研究 | 497篇 |
出版年
2013年 | 236篇 |
2012年 | 440篇 |
2011年 | 1020篇 |
2010年 | 201篇 |
2009年 | 141篇 |
2008年 | 534篇 |
2007年 | 653篇 |
2006年 | 602篇 |
2005年 | 623篇 |
2004年 | 579篇 |
2003年 | 601篇 |
2002年 | 549篇 |
2001年 | 1102篇 |
2000年 | 1089篇 |
1999年 | 611篇 |
1992年 | 597篇 |
1991年 | 457篇 |
1990年 | 524篇 |
1989年 | 508篇 |
1988年 | 487篇 |
1987年 | 482篇 |
1986年 | 508篇 |
1985年 | 574篇 |
1984年 | 453篇 |
1983年 | 405篇 |
1982年 | 357篇 |
1981年 | 381篇 |
1980年 | 428篇 |
1979年 | 973篇 |
1978年 | 778篇 |
1977年 | 784篇 |
1976年 | 607篇 |
1975年 | 660篇 |
1974年 | 989篇 |
1973年 | 758篇 |
1972年 | 758篇 |
1971年 | 938篇 |
1970年 | 1188篇 |
1969年 | 938篇 |
1968年 | 875篇 |
1967年 | 858篇 |
1966年 | 774篇 |
1965年 | 560篇 |
1959年 | 321篇 |
1958年 | 499篇 |
1957年 | 333篇 |
1956年 | 271篇 |
1955年 | 270篇 |
1954年 | 254篇 |
1948年 | 168篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
141.
The in vivo expression of the globin genes of theβ cistron in γ-,δ-, andδβ-thalassemia heterozygotes
A. J. Dimovski A. D. Adekile T. H. J. Huisman 《Cellular and molecular life sciences : CMLS》1994,50(2):167-170
There is considerable evidence suggesting that the switch from to and chain production after birth is due, in part, to silencing of the genes by stage-specific factors which bind to their promoters and to the competition from the adult ( and ) genes for a common enhancer element located in the locus control region. As a consequence one can expect that the increased Hb F production in adults with hereditary persistence of fetal hemoglobin or -thalassemia is directed mainly by -globin genes in cis to the deletion(s) responsible for these conditions. Here we review data on heterozygotes with -, -, or -thalassemia, who also had anAT mutation, in cis or in trans, which was used as a marker of gene expression. The results show that a deletion affecting adult genes favors the expression of genes in cis, while the deletion of a single gene does not affect the expression of the gene in cis but leads to a faster switch postnatally. 相似文献
142.
Highly active metabolites have been detected in the hemolymph of the lepidopteranSpodoptera exigua infected with the mycopathogen,Beauveria bassiana. A combination of phenyl sepharose and CM ion exchange chromatography was utilized to extract the active metabolites from infected hemolymph samples. The active in vivo metabolites, having a molecular mass greater than 10 KDa, were thermolabile and were inactivated by proteinase K. These metabolites were characterized by their ability to disrupt metamorphosis, killing treated larvae at the wandering or pupal stage. Additionally, injection ofS. exigua larvae with active samples caused a reduction in the number of filopodial-producing hemocytes. The biological activities and biochemical properties suggest that novel compounds are produced duringB. bassiana mycosis. 相似文献
143.
144.
P. Paoli G. Camici G. Manao G. Ramponi 《Cellular and molecular life sciences : CMLS》1995,51(1):57-62
A new aromatic acyl phosphate, 2-methoxybenzoyl phosphate, has been synthesized. The compound shows an intrinsic fluorescence; it displays an intense emission band at 390 nm upon excitation in the near UV region. This band practically disappears after hydrolysis of the product. On the other hand, the product displays differences in the near UV absorption spectra measured before and after hydrolysis. The at 301 nm is 2720 M–1 cm–1, a value that is 4.3-fold higher than that of benzoyl phosphate (the usual substrate for acylphosphatase assay) at 283 nm. The main kinetic parameters of three different acylphosphatase molecular forms (the muscular isoenzyme and two subtypes of the organ common isoenzyme) were determined using both benzoyl phosphate and 2-methoxybenzoyl phosphate as substrates, and then compared. These kinetic data and the UV absorption and fluorescence properties of 2-methoxybenzoyl phosphate sugest that this compound has better substrate features than benzoyl phosphate, and can be used for both high sensitivity continuous fluorimetric and UV absorption spectrophotometric assays of acylphosphatase. 相似文献
145.
J. Martín-Gil F. J. Martín-Gil G. Delibes-de-Castro P. Zapatero-Magdaleno F. J. Sarabia-Herrero 《Cellular and molecular life sciences : CMLS》1995,51(8):759-761
Vermillion has been shown to be useful in preserving human bones from 5000 years ago. Remarkably well-preserved human bones have been found in the dolmenic burial La Velilla in Osorno (Palencia, Spain), carefully covered by pulverized cinnabar (vermillion) which ensured their preservation even in non-favorable climatic conditions. We believe the red powder was deliberately deposited for preservative use because no cinnabar mine is to be found within 160 km, because of the large amount (hundreds of kilograms) used, and because its composition, red mercuric sulphide, is similar to that of preparations used in technical embalming. This finding pushes back the data of the use of mercury ore for preservation by four millennia in South America, and by at least one millennium in the Old World. Chemical and thermal analyses of vermillion in La Velilla have demonstrated its great purity and shown that the cinnabar was pulverized and washed (but not heated), producing a bright red-orange tone. 相似文献
146.
X. Wang C. A. Lessman D. B. Taylor T. K. Gartner 《Cellular and molecular life sciences : CMLS》1995,51(11):1097-1102
Gastrulation is characterized by dramatic cell migration which is thought to require the interaction of cell adhesion molecules with extracellular molecules. We have tested two novel peptides, a fibronectin peptide and a fibronectin receptor peptide, for their effects on gastrulation of the leopard frogRana pipiens. The fibronectin peptide DRVPHSRNSIT corresponds to residues 1373–1383 of the cell-binding domain of fibronectin; the receptor peptide DLYYLMDL corresponds to residues 124–131 of 1 subunit of a variety of integrins including 51. Either of these peptides significantly inhibited gastrulation after being microinjected into mid-blastulae. These results indicate that these sequences may correspond to the ligand/receptor interaction sites of fibronectin and its receptor(s). 相似文献
147.
The composition of the Earth's early atmosphere is a subject of continuing debate. In particular, it has been suggested that elevated concentrations of atmospheric carbon dioxide would have been necessary to maintain normal surface temperatures in the face of lower solar luminosity in early Earth history. Fossil weathering profiles, known as palaeosols, have provided semi-quantitative constraints on atmospheric oxygen partial pressure (pO2) before 2.2 Gyr ago. Here we use the same well studied palaeosols to constrain atmospheric pCO2 between 2.75 and 2.2 Gyr ago. The observation that iron lost from the tops of these profiles was reprecipitated lower down as iron silicate minerals, rather than as iron carbonate, indicates that atmospheric pCO2 must have been less than 10(-1.4) atm--about 100 times today's level of 360 p.p.m., and at least five times lower than that required in one-dimensional climate models to compensate for lower solar luminosity at 2.75 Gyr. Our results suggest that either the Earth's early climate was much more sensitive to increases in pCO2 than has been thought, or that one or more greenhouse gases other than CO2 contributed significantly to the atmosphere's radiative balance during the late Archaean and early Proterozoic eons. 相似文献
148.
采用雪崩热电子注入技术和高频C-V准静态C-V特性测试,研究了新型快速热氮化的SiOxNy介质膜界面陷阱的特征,侧重于研究界面陷阱的特性与分布。结果表明:这种SiOxNy薄膜禁带中央界面陷阱密度随氮化时间的分布变化呈现”回转效应“,且存在着不同类型、密度悬殊很大的电子陷阱、指出雪崩热电子注入过程中在Si/SiOxNy界面上产生两种性质不同的快界面态陷阱;给出了这两种界面态陷阱密度在禁带中能量的分布 相似文献
149.
The smectite to illite transformation in active geothermal systems of New Zealand can be simulated by a first-order reaction
kinetic model, which provides direct estimates about the minumum ages of active geothermal systems themselves. The derived
kinetic values show that the smectite to illite transformation is sensitive to both temperature and time. 相似文献
150.