Über den Einbau von Anthranilsäure in Peganin

Summary Anthranilic acid-(¹⁴COOH) was administered to rooted leaves ofAdhatoda vasica Nees. The isolated alkaloid peganine = vasicine was degraded according to the method ofSpäth andHikawitz (Figure). The anthranilic acid obtained showed the same specific radioactivity as the alkaloid. Therefore anthranilic acid must be regarded as a direct precursor of peganine inA. vasica Nees.     

Protein and lysozyme content of adult human nucleus pulposus

Summary A radiologically normal human nucleus pulposus was extracted with 4 M guanidinium chloride and the non-collagenous proteins separated from the proteoglycans by dissociative density gradient centrifugation. Lysozyme was identified as a matrix constituent of the normal, mature human nucleus pulposus.Supported by Public Health Service Training Grant ES-07088.     

A new semisynthetic ergot peptide alkaloid: DCN 203-922

Summary We report the synthesis, stereochemistry and preliminary pharmacological evaluation of DCN 203-922, a novel ergot alkaloid of the cyclol type, which contains in its peptide moiety the uncommon amino acid L-allo-isoleucine.Part of this paper was reported by this author at the Herbstversammlung der Schweizerischen Chemischen Gesellschaft, Bern, in October 1986.     

Volatile constituents of wolf (Canis lupus) urine as related to gender and season

Summary The volatile constituents of wolf urine were examined via capillary gas chromatography and compared among male, female, and castrate male. Several compounds including methyl isopentyl sulfide, 3,5-dimethyl-2-octanone, and acetophenone were clearly associated with the gender of the animal and many displayed a seasonal dependence. In addition, 2 long-chain aldehydes isolated from urine samples by an HPLC procedure also correlated with the endocrine status of the animal.This work was partially funded by the US Fish and Wildlife Service.We thank Dr Sharon L. Smith of IBM Instruments, Danbury, Connecticut, for recording spectra of some wolf urinary constitutents on an IBM Fourier-transform IR spectrometer.No such compounds were observed in any other animal species during our previous work with several mammals.     

HCO 3 − -ATPase activity distribution in rat liver cell fractions prepared by zonal centrifugation

Summary Plasma membrane sheets prepared by zonal centrifugation of a premicrosomal pellet obtained from a rat liver homogenate are devoid of HCO ₃ ^– -ATPase activity. Since the microsomal fraction is also lacking in this ATPase activity, it can be concluded that the HCO ₃ ^– -ATPase is not involved in the secretion of HCO ₃ ^– into bile. Acknowledgments. This investigation was supported in part by grants No. DE-02600 and AM 80686 from the United States Public Health Service. The A-XII zonal rotor is used under subcontract No. 3796 with the Union Carbide Corporation.