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41.
Structure of the detoxification catalyst mercuric ion reductase from Bacillus sp. strain RC607 总被引:10,自引:0,他引:10
Several hundred million tons of toxic mercurials are dispersed in the biosphere. Microbes can detoxify organo-mercurials and mercury salts through sequential action of two enzymes, organomercury lyase and mercuric ion reductase (MerA). The latter, a homodimer with homology to the FAD-dependent disulphide oxidoreductases, catalyses the reaction NADPH + Hg(II)----NADP+ + H+ + Hg(0), one of the very rare enzymic reactions with metal substrates. Human glutathione reductase serves as a reference molecule for FAD-dependent disulphide reductases and between its primary structure and that of MerA from Tn501 (Pseudomonas), Tn21 (Shigella), p1258 (Staphylococcus) and Bacillus, 25-30% of the residues have been conserved. All MerAs have a C-terminal extension about 15 residues long but have very varied N termini. Although the enzyme from Streptomyces lividans has no addition, from Pseudomonas aeruginosa Tn501 and Bacillus sp. strain RC607 it has one and two copies respectively of a domain of 80-85 residues, highly homologous to MerP, the periplasmic component of proteins encoded by the mer operon. These domains can be proteolytically cleaved off without changing the catalytic efficiency. We report here the crystal structure of MerA from the Gram-positive bacterium Bacillus sp. strain RC607. Analysis of its complexes with nicotinamide dinucleotide substrates and the inhibitor Cd(II) reveals how limited structural changes enable an enzyme to accept as substrate what used to be a dangerous inhibitor. Knowledge of the mode of mercury ligation is a prerequisite for understanding this unique detoxification mechanism. 相似文献
42.
43.
尚翠平 《科技情报开发与经济》2004,14(12):224-225
论述了普通机床数控改造的可行性、必要性和经济性,并对C620-1型普通机床的数控改造过程进行了较详细的总结和论述,对普通机床的数控改造具有实际的指导作用。 相似文献
44.
21世纪党校图书馆工作面临的挑战与对策 总被引:2,自引:0,他引:2
刘翠萍 《科技情报开发与经济》2003,13(12):66-67
针对21世纪党校图书馆工作面临的挑战与目前的现状,提出了适应时代新要求的党校图书馆工作管理的对策。 相似文献
45.
崔新军 《济南大学学报(自然科学版)》2003,17(2):163-164
提出了利用电感耦合等离子发射光谱ICP -AES法测定原油中铁、钙元素含量的方法 ,考察了雾化压力、RF功率等对被测元素分析线强度的影响 ,确定了仪器的最佳使用条件。试验表明 ,该方法简便、快速、精密度和准确度均令人满意 ,能满足实际应用的要求 相似文献
46.
LANE RE 《Advancement of science》1948,5(18):96-118
47.
Objective: This study is aimed at describing the clinical outcome of amniotic membrane transplantation for exposure of porous sphere implants. Methods: A retrospective review of consecutive cases of porous sphere orbital implant exposure was carried out. Eight cases were presented between May 2004 and Oct. 2006 (5 males, 3 females; mean age 44.5 years). Six had enucleation and two had evisceration. Exposure occurred in two primary and six secondary. Orbital implant diameter was 22 mm in seven cases and 20 mm in one case. Six patients are with hydroxyapatite and two with high-density porous polyethylene (Medpor) orbital implants. The mean time from implantation to exposure was 1.1 months (range 0.8~2 months). All patients required surgical intervention. Results: The time of follow-up ranged from 3.0 to 28.0 months (mean 16.5 months). Amniotic membrane grafting successfully closed the defect without re-exposure in all of these patients. The grafts were left bare with a mean time to conjunctiva of about 1 month (range 0.8~1.5 months). Conclusion: Exposed porous sphere implants were treated successfully with amniotic membrane graft in all of patients. The graft is easy to harvest. This technique is useful, dose not lead to prolonged socket inflammation and infection, and it is valuable application extensively. 相似文献
48.
E. Häfliger 《Cellular and molecular life sciences : CMLS》1948,4(6):223-225
Summary The action of DDT on honey-bees depends to a high extent on the temperature (see graph). At 36°C (breeding temperature) DDT has a far weaker insecticidal action than at 20°C (laboratory temperature). This shows that the insecticidal properties of DDT diminish with the raise of temperature.This resistance to DDT at higher temperature is most propitious for bee-keeping and also explains the fact why in agricultural practice there has been no corroborated case of poisoning of bees, though in laboratory tests DDT avered itself to be toxic to bees. 相似文献
49.
陶娥 《长春师范学院学报》2007,(3)
由于民间文学的社会功能是多方面的,东北地区的民间文学在东北旅游文化中能起到传播知识、净化灵魂、陶冶情操、娱乐休闲等教益作用。 相似文献
50.