全文获取类型
收费全文 | 31537篇 |
免费 | 88篇 |
国内免费 | 133篇 |
专业分类
系统科学 | 317篇 |
丛书文集 | 558篇 |
教育与普及 | 78篇 |
理论与方法论 | 90篇 |
现状及发展 | 14242篇 |
研究方法 | 1282篇 |
综合类 | 14862篇 |
自然研究 | 329篇 |
出版年
2013年 | 185篇 |
2012年 | 421篇 |
2011年 | 768篇 |
2010年 | 189篇 |
2008年 | 536篇 |
2007年 | 642篇 |
2006年 | 587篇 |
2005年 | 594篇 |
2004年 | 540篇 |
2003年 | 607篇 |
2002年 | 512篇 |
2001年 | 1079篇 |
2000年 | 1069篇 |
1999年 | 627篇 |
1994年 | 366篇 |
1992年 | 613篇 |
1991年 | 455篇 |
1990年 | 554篇 |
1989年 | 495篇 |
1988年 | 452篇 |
1987年 | 518篇 |
1986年 | 529篇 |
1985年 | 640篇 |
1984年 | 466篇 |
1983年 | 429篇 |
1982年 | 397篇 |
1981年 | 428篇 |
1980年 | 440篇 |
1979年 | 1063篇 |
1978年 | 819篇 |
1977年 | 788篇 |
1976年 | 642篇 |
1975年 | 665篇 |
1974年 | 933篇 |
1973年 | 795篇 |
1972年 | 719篇 |
1971年 | 862篇 |
1970年 | 1135篇 |
1969年 | 945篇 |
1968年 | 888篇 |
1967年 | 878篇 |
1966年 | 775篇 |
1965年 | 569篇 |
1959年 | 325篇 |
1958年 | 505篇 |
1957年 | 340篇 |
1956年 | 313篇 |
1955年 | 292篇 |
1954年 | 288篇 |
1948年 | 200篇 |
排序方式: 共有10000条查询结果,搜索用时 9 毫秒
761.
五峰垃圾填埋场渗滤液污染特征及处理系统 总被引:1,自引:0,他引:1
系统分析了五峰垃圾填埋场渗滤液中COD,BOD_5的季节变化特征,并对该填埋场的污水处理系统进行了研究和评价。 相似文献
762.
763.
Calcium/calmodulin-dependent protein kinase II increases glutamate and noradrenaline release from synaptosomes 总被引:28,自引:0,他引:28
A variety of evidence indicates that calcium-dependent protein phosphorylation modulates the release of neurotransmitter from nerve terminals. For instance, the injection of rat calcium/calmodulin-dependent protein kinase II (Ca2+/CaM-dependent PK II) into the preterminal digit of the squid giant synapse leads to an increase in the release of a so-far unidentified neurotransmitter induced by presynaptic depolarization. But until now, it has not been demonstrated that Ca2+/CaM-dependent PK II can also regulate neurotransmitter release in the vertebrate nervous system. Here we report that the introduction of Ca2+/CaM-dependent PK II, autoactivated by thiophosphorylation, into rat brain synaptosomes (isolated nerve terminals) increases the initial rate of induced release of two neurotransmitters, glutamate and noradrenaline. We also show that introduction of a selective peptidergic inhibitor of Ca2+/CaM-dependent PK II inhibits the initial rate of induced glutamate release. These results support the hypothesis that activation of Ca2+/CaM-dependent PK II in the nerve terminal removes a constraint on neurotransmitter release. 相似文献
764.
R D Salter R J Benjamin P K Wesley S E Buxton T P Garrett C Clayberger A M Krensky A M Norment D R Littman P Parham 《Nature》1990,345(6270):41-46
Adhesion measurements between CD8 and 48 point mutants of HLA-A2.1 show that the CD8 alpha-chain binds to the alpha 3 domain of HLA-A2.1. Three clusters of alpha 3 residues contribute to the binding, with an exposed, negatively charged loop (residues 223-229) playing a dominant role. CD8 binding correlates with cytotoxic T-cell recognition and sensitivity to inhibition by anti-CD8 antibodies. Impaired alloreactive T-cell recognition of an HLA-A2.1 mutant with reduced affinity for CD8 is not restored by functional CD8 binding sites on an antigenically irrelevant class I molecule. Therefore, complexes of CD8 and the T-cell receptor bound to the same class I major histocompatibility complex molecule seem to be necessary for T-cell activation. 相似文献
765.
New Sivapithecus humeri from Pakistan and the relationship of Sivapithecus and Pongo 总被引:2,自引:0,他引:2
New humeri of two species of the Miocene hominoid Sivapithecus are described from near Chinji in Pakistan from between approximately 9 and 11 Myr ago. Sivapithecus, a middle and late Miocene hominoid from Turkey and Indo-Pakistan, is overall unlike any living hominoid, although facial-palatal similarities to the extant orangoutan, Pongo, have been used to support a hypothesis of close relationship. Living hominoids have postcranial similarities assumed to be shared derived, among them features of the proximal humerus. However, the new Sivapithecus proximal humeri differ from those of living hominoids, supporting an alternative hypothesis in which Sivapithecus and Pongo are not closely related. It is not clear how to choose between these incompatible hypotheses. 相似文献
766.
767.
A mutation in the tRNA(Leu)(UUR) gene associated with the MELAS subgroup of mitochondrial encephalomyopathies 总被引:90,自引:0,他引:90
Mitochondrial encephalomyopathies are usually divided into three distinct clinical subgroups: (1) mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS); (2) myoclonus epilepsy associated with ragged-red fibres (MERRF); and (3) chronic progressive external ophthalmoplegia (CPEO) including Kearns-Sayre syndrome. Large deletions of human mitochondrial DNA and a transition mutation at the mitochondrial transfer RNALys gene give rise to CPEO including Kearns-Sayre syndrome and MERRF, respectively. Here we report an A-to-G transition mutation at nucleotide pair 3,243 in the dihydrouridine loop of mitochondrial tRNA(Leu)(UUR) that is specific to patients with MELAS. Because this mutation creates an ApaI restriction site, we could perform a simple molecular diagnostic test for the disease. The mutation was present in 26 out of 31 independent MELAS patients and 1 out of 29 CPEO patients, but absent in the 5 MERRF and 50 controls tested. Southern blot analysis confirmed that the mutant DNA always coexists with the wild-type DNA (heteroplasmy). 相似文献
768.
The neurons of layer 4 in the adult cerebral cortex receive their major ascending inputs from the thalamus. In development, however, thalamic axons arrive at the appropriate cortical area long before their target layer 4 neurons have migrated into the cortical plate. The axons accumulate and wait in the zone below the cortical plate, the subplate, for several weeks before invading the cortical plate. The subplate is a transient zone that contains the first postmitotic neurons of the telencephalon. These neurons mature well before other cortical neurons, and disappear by cell death after the thalamic axons have grown into the overlying cortical plate. The close proximity of growing thalamocortical axons and subplate neurons suggests that they might be involved in interactions important for normal thalamocortical development. Here we show that early in development the deletion of subplate neurons located beneath visual cortex prevents axons from the lateral geniculate nucleus of the thalamus from recognizing and innervating visual cortex, their normal target. In the absence of subplate neurons, lateral geniculate nucleus axons continue to grow in the white matter past visual cortex despite the presence of their target layer 4 neurons. Thus the transient subplate neurons are necessary for appropriate cortical target selection by thalamocortical axons. 相似文献
769.
Acute leukaemia in bcr/abl transgenic mice 总被引:38,自引:0,他引:38
N Heisterkamp G Jenster J ten Hoeve D Zovich P K Pattengale J Groffen 《Nature》1990,344(6263):251-253
770.
In the host cell, retroviral DNAs exist in three main forms: unintegrated linear, unintegrated circular, and integrated (the provirus). High levels of unintegrated forms of retroviral DNA often correlate with superinfection and accompanying cytopathic effects, as, for example, in the case of feline acquired immunodeficiency. In culture, HIV-1 infection also results in high levels of unintegrated viral DNA although direct correlations with cytopathicity have not been made. The low frequency of HIV-1-infected cells in patients has made it difficult to determine the structure of the viral DNA in fresh tissue samples from AIDS patients by standard methods such as Southern hybridization. The PCR technique however, which allows the detection of viral DNA at levels far below that possible by other hybridization methods is, in its conventional form, of limited use for quantitative analysis. To study the amount and form of HIV-1 DNA in primary tissue of AIDS patients we have therefore modified the PCR method. Our results indicate that each of the three species of viral DNA are detectable in blood and brain of AIDS patients, and that in autopsy samples from patients with HIV encephalitis there is a considerably higher proportion of unintegrated viral DNA. 相似文献