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11.
Résumé Des essais réitérés ont montré aux auteurs que la protéine extraite des racines d'Allium cepa, deVicia faba et dePisum sativum est identifiable à l'histone. L'analyse de l'amine acide des hydrolysates des histones ne révèle pas de différences entre les trois espèces végétales citées et le thymus de veau. Dans les végétaux, cependant, le rapport lysine-arginine est plus élevé.
The research was partially supported by the US Atomic Energy Commission under Contract AT(11-1)826. 相似文献
The research was partially supported by the US Atomic Energy Commission under Contract AT(11-1)826. 相似文献
12.
Correlation between the ATPase and microtubule translocating activities of sea urchin egg kinesin 总被引:2,自引:0,他引:2
Coupling between ATP hydrolysis and microtubule movement was demonstrated several years ago in flagellar axonemes and subsequent studies suggest that the relevant microtubule motor, dynein, uses ATP to drive microtubule sliding by a cross-bridge mechanism analogous to that of myosin in muscles. Kinesin, a microtubule-based motility protein which may participate in organelle transport and mitosis, binds microtubules in a nucleotide-sensitive manner, and requires hydrolysable nucleotides to translocate microtubules over a glass surface. Recently, neuronal kinesin was shown to possess microtubule-activated ATPase activity although coupling between ATP hydrolysis and motility was not demonstrated. Here we report that sea urchin egg kinesin, prepared either with or without a 5'-adenylyl imidodiphosphate(AMPPNP)-induced microtubule binding step, also possesses significant microtubule-activated ATPase activity when Mg-ATP is used as a substrate. This ATPase activity is inhibited in a dose-dependent manner by addition of Mg-free ATP, by chelation of Mg2+ with EDTA, by addition of Na3VO4, or by addition of AMPPNP with or without Mg2+. Addition of these same reagents also inhibits the microtubule-translocating activities of sea urchin egg kinesin in a dose-dependent manner, supporting the hypothesis that kinesin-driven motility is coupled to the microtubule-activated Mg2+-ATPase activity. 相似文献
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Anti-nucleic acid specificites of mouse myeloma immunoglobulins 总被引:5,自引:0,他引:5
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W Bruening N Bardeesy B L Silverman R A Cohn G A Machin A J Aronson D Housman J Pelletier 《Nature genetics》1992,1(2):144-148
Denys-Drash syndrome is a rare human developmental disorder affecting the urogenital system and leading to renal failure, intersex disorders and Wilms' tumour. In this report, four individuals with this syndrome are described carrying germline point mutations in the Wilms' tumour suppressor gene, WT1. Three of these mutations were in the zinc finger domains of WT1. The fourth occurred within intron 9, preventing splicing at one of the alternatively chosen splice donor sites of exon 9 when assayed in vitro. These results provide genetic evidence for distinct functional roles of the WT1 isoforms in urogenital development. 相似文献
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Submucosal glands are the predominant site of CFTR expression in the human bronchus. 总被引:19,自引:0,他引:19
J F Engelhardt J R Yankaskas S A Ernst Y Yang C R Marino R C Boucher J A Cohn J M Wilson 《Nature genetics》1992,2(3):240-248
We have used in situ hybridization and immunocytochemistry to characterize the cellular distribution of cystic fibrosis (CF) gene expression in human bronchus. The cystic fibrosis transmembrane conductance regular (CFTR) was primarily localized to cells of submucosal glands in bronchial tissues from non-CF individuals notably in the serous component of the secretory tubules as well as a subpopulation of cells in ducts. Normal distribution of CFTR mRNA was found in CF tissues while expression of CFTR protein was genotype specific, with delta F508 homozygotes demonstrating no detectable protein and compound heterozygotes expressing decreased levels of normally distributed protein. Our data suggest mechanisms whereby defects in CFTR expression could lead to abnormal production of mucus in human lung. 相似文献
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Heterozygous mutations in the gene encoding noggin affect human joint morphogenesis 总被引:10,自引:0,他引:10
Gong Y Krakow D Marcelino J Wilkin D Chitayat D Babul-Hirji R Hudgins L Cremers CW Cremers FP Brunner HG Reinker K Rimoin DL Cohn DH Goodman FR Reardon W Patton M Francomano CA Warman ML 《Nature genetics》1999,21(3):302-304
The secreted polypeptide noggin (encoded by the Nog gene) binds and inactivates members of the transforming growth factor beta superfamily of signalling proteins (TGFbeta-FMs), such as BMP4 (ref. 1). By diffusing through extracellular matrices more efficiently than TGFbeta-FMs, noggin may have a principal role in creating morphogenic gradients. During mouse embryogenesis, Nog is expressed at multiple sites, including developing bones. Nog-/- mice die at birth from multiple defects that include bony fusion of the appendicular skeleton. We have identified five dominant human NOG mutations in unrelated families segregating proximal symphalangism (SYM1; OMIM 185800) and a de novo mutation in a patient with unaffected parents. We also found a dominant NOG mutation in a family segregating multiple synostoses syndrome (SYNS1; OMIM 186500); both SYM1 and SYNS1 have multiple joint fusion as their principal feature. All seven NOG mutations alter evolutionarily conserved amino acid residues. The findings reported here confirm that NOG is essential for joint formation and suggest that NOG requirements during skeletogenesis differ between species and between specific skeletal elements within species. 相似文献