Generation of Linear and Parabolic Concentration Gradients by Using a Christmas Tree-Shaped Microfluidic Network

This paper describes a simple method of generating concentration gradients with linear and parabolic profiles by using a Christmas tree-shaped microfluidic network.The microfluidic gradient generator consists of two parts:a Christmas tree-shaped network for gradient generation and a broad microchannel for detection.A two-dimensional model was built to analyze the flow field and the mass transfer in the microfluidic network.The simulating results show that a series of linear and parabolic gradient profiles were generated via adjusting relative flow rate ratios of the two source solutions(R_L~2≥0.995 and _PR~2≥0.999),which could match well with the experimental results(R_L~2≥0.987 and _PR~2≥0.996).The proposed method is promising for the generation of linear and parabolic concentration gradient profiles,with the potential in chemical and biological applications such as combinatorial chemistry synthesis,stem cell differentiation or cytotoxicity assays.     

三七多糖的微波辅助提取工艺

采用正交试验法优化了三七多糖的微波提取工艺,采用激光粒度分析法探讨了提取机理,分析了多糖的单糖组成,并测定了多糖的溶解性。三七多糖的较佳微波辅助提取工艺条件为微波处理时间8min、浸泡时间60min、液固比30mL/g、粒度200目。微波辅助提取的多糖提取率(21.0mg/g)显著高于传统提取(12.7mg/g)。三七多糖的Molish反应呈阳性,多糖酸水解产物的薄层色谱呈现出3个斑点,分别与葡萄糖、半乳糖和阿拉伯糖相对应。微波辅助提取对三七多糖的提取效果明显优于传统提取,其机理可能是微波处理促进了三七样品的水化和溶胀过程,使样品结构变得松散、膨胀,从而促进了多糖从样品中扩散和溶出。三七多糖主要由葡萄糖、半乳糖和阿拉伯糖3种单糖构成,pH值对其溶解性影响较大。     

Synthesis and luminescence properties of Mn4+-dopant Ca14Zn6Ga10−xAlxO35 solid solution

Mn~(4+)-activated oxide phosphors,owing to their desirable spectral features,eco-friendly and low cost,are emerging as a new class of non-rare-earth red phosphors for warm white LEDs.However,these phosphors possess low photoluminescence quantum efficiency excited by blue chip currently.Herein we report an isostructural solid solution of Ca_(14)Zn_6Ga_(10-x)Al_xO_(35):0.15Mn~(4+)(0≤x≤10)synthesized by a traditional solidstate reaction route.The microstructure and luminescent performance of this red-emitting phosphor are investigated in detail with the aids of X-ray diffraction,diffuse reflection spectra,photoluminescence spectra/decay/QE,and temperature-dependent PL/QE measurements.Blue shift of energy peaks of~4A_2→~4T_1and~4A_2→~4T_2transition is illustrated by the Tanabe–Sugano diagram and the configurational coordinate diagram.The crystal field strength(Dq)and the Racah parameters(B and C)are carefully calculated to estimate the nephelauxetic effectβrespectively.Particularly we achieve external and internal quantum efficiencies as high as26.1%and 40.3%for Ca_(14)Zn_6Ga_6Al_4O_(35):0.15Mn~(4+)excited by 466 nm,the highest one ever reported in Mn~(4+)activated oxide phosphors under the similar condition.     

Nanoengineering of amino - functionalized mesoporous silica nanospheres as nanoreactors

Selective functionalization of mesoporous silica nanospheres (MSNs) is crucial for nanoengineering of MSNs. Herein, we have combined “surface-protected etching strategy” and “cationic surfactant assisted etching strategy” to prepare functionalized MSNs with externally attached amino groups. The externally attached NH2 groups endow the catalysts with excellent catalytic performance for nitroaldol reaction between nitromethane and benzaldehyde. In addition, those NH2-MSNs can also be used to support gold nanoparticles, which display very good catalytic performance for reduction of 4-nitrophenol. It can be envisioned that the synthesis protocol developed in this work could also be extended to nanoengineered MSNs, which provides opportunities for nanoreactors design.     

论政府信息资源的开发

论述了政府信息的内容、特点及其如何搜集检索,指出开发我国政府信息资源应从宏观调控、政策法规、信息公开等5个方面进行。     

广西山口红树林自然保护区蝶类资源考察报告

广西山口红树林自然保护区的蝶类有7科30属46种,以粉蝶科、蛱蝶科的种类较多,分别占总种类数的26.1%和23.9%.不同季节的蝴蝶种类和数量分布不均匀,春、夏、秋、冬季的蝴蝶种类数分别为15种、36种、39种和11种,捕获的数量分别占18.6%、32.2%、40.9%和8.3%.春季常见的蝶类为美目蛱蝶(Junonia almana almana)、珐蛱蝶指名亚种(Phalanta phalantha phalantha)、橙粉蝶(Ixias pyrene)和达摩凤蝶(Popilio demoleus)等;夏季常见蝶类为酢浆小灰蝶指名亚种(Pseudozizeeria maha maha)、幻紫斑蛱蝶海南亚种(Hypolimnas bolina kezia)、波蛱蝶(Ariadne ariadne)和青粉蝶海南亚种(Pareronia valeria hainanensis)等;秋季常见蝶类为虎斑蝶指名亚种(Danaus genutia genutia)、青粉蝶海南亚种(Pareronia valeria hainanensis)、紫灰蝶(Chilades lajus)和黑脉圆粉蝶指名亚种(Cepora nerissa nerissa)等;冬季的常见种为黑脉圆粉蝶指名亚种、无标黄粉蝶云南亚种(Eurema brigitta yunnana)和达摩凤蝶(Popilio demoleus)等.仅有6种蝴蝶可在一年四季采集到.区系成分以东洋种为主,占60.9%,东洋古北兼有种占36.9%,广布种仅占2.2%.蝴蝶群落多样性指数以秋季为最高.     

小城镇的发展与乡村城市化

界定了乡村城市化的内涵,分析了中国小城镇形成过程及其功能特征,认为小城镇的发展将会对农业人口转移、农村经济发展、生产技术、社会文化习俗、建筑风络等产生重要影响,并极大地促进城市化进程,最终实现城乡一体化目标。     

硫酸庆大霉素聚乳酸微球的制备及其体外缓释度的测定

以硫酸庆大霉素为囊心物质,采用复乳法制备了硫酸庆大霉素-聚乳酸微球（gentamycin sulfate-polylactic acid-microspheres,GTMS-PLA-MS）,比较了不同制备条件下所得微球的差异.扫描电镜（SEM）测试表明微球外观呈球形并且多孔,两种条件下制备的微球平均粒径分别为6.67 μm和11.70μm,药物包封率分别为37.52%和49.19%.用差热分析法（DTA）分析微球得知,硫酸庆大霉素与聚乳酸两种物质相互融为一体.以pH=7.4的磷酸盐缓冲溶液为释药介质,用紫外分光光度法（UV）对载药微球的体外释药过程进行了试验.结果表明,微球在最初20 min有突释效应,此后是缓慢释药,最终累积释药量达95%以上.     

MAHIS-based analysis and design of financial investment planning system

In financial investment planning, a large number of components that interact in varying and complex ways are involved. This leads to complex behavior that is difficult to understand, predict and manage. A single intelligent technique can not solve the complicated and elaborate investment planning problems. It is necessary to study those problems synthetically by combining the multiple intelligent techniques. We employed fussy algorithms, genetic algorithms, etc. to solve complicated financial portfolio management in this paper. We analyse and design an agent-based hybrid intelligent system by following the methodology for constructing agent-based hybrid intelligent system (MAHIS). The system starts with the financial risk tolerance evaluation based on fussy algorithms. Asset allocation, portfolio selections, interest predictions, and ordered weighted averaging can be conducted by using hybrid intelligent techniques. The planning agent in the system can easily access all the intelligent processing agents, including the agents of financial risk tolerance assessment, asset allocation, portfolio selection, interest prediction, and decision aggregation. Overall system robustness is facilitated.     

重组人内皮抑素层析复性的相关研究

研究了由大肠杆菌表达的重组人内皮抑素(Recombinant human endostatin,rhES)的复性、纯化过程.比较先层析复性后纯化(工艺1)和先纯化后层析复性(工艺2)两种工艺的结果表明:工艺1所得rhES的收率和活性回收率分别为64.44%和213.19%,与工艺2相比分别提高8.70%和33.04%.工艺1中,研究了3种凝胶过滤柱复性及复性pH和上样蛋白浓度对复性的影响,并优化相关工艺.结果表明:在pH9.0,rhES浓度3.91 mg/mL的条件下,采用双梯度凝胶过滤Sephacryl S-100 HR进行层析复性,Sephadex G-25脱盐后,用SP Sepharose FF阳离子交换吸附纯化,获得活性回收率为213.19%,HPLC纯度为97.07%的rhES,其IC50为1.58 ug/mL.