Overexpression and export of Vibrio anguillarum metalloprotease in Escherichia coli

Vibrio anguillarum metalloprotease, an extracellular zinc metalloprotease involved in the virulence mechanism of Vibrio anguillarum, is synthesized from the empA gene as a 611-residue precursor and naturally secreted via Sec secretion pathway in Vibrio anguillarum. In this study, heterologous expression of the empA gene encoding metalloprotease and export of the recombinant metalloprotease in Escherichia coli were examined. The empA gene was subcloned into pBAD24 with arabinose promoter and sequenced. The sequence encoded a polypeptide (611 amino acids) consisting of four domains: a signal peptide, an N-terminal propeptide, a mature region and a C-terminal propeptide. The empA gene inserted in plasmid pBAD24 was overexpressed in TOP10 strain of E. coli after arabinose induction. The 36kDa polypeptide of the recombinant metalloprotease as the mature protease was further confirmed by SDS-PAGE and immunoblotting. It was found that recombinant metalloprotease with the EmpA activity and antigenicity was exported into the periplasm of Escherichia coli cells via Sec translocation pathway, whereas it was secreted into extracellular environments in V. anguillarum. The results imply that the expression, export and processing mechanism of the protein in E. coli are similar to those in V. anguillarum.     

通信原理实验系统的设计与实现

当前的通信原理实验多为纸质版实验报告,需要学员将实验结果、信号波形进行徒手绘制,效率不高;且纸质实验报告还不便于档案的保存和管理。从提高实验课堂效率和档案记录的数字化存贮、管理角度出发,开发了通信原理实验系统。教学实践表明,该系统能满足实验报告计算机存贮、管理的功能,但有待进一步完善。     

影响机械加工精度的主要因素

文章介绍了影响机械加工精度的几个重要因素。在诸多误差因素中,机床的几何误差、工艺系统的受力变形和受热变形占有突出的位置,通过了解这些误差因素是如何影响加工误差的,可以使工件的加工达到质量要求。     

基于VB与MO的120急救系统的设计与开发

120急救在现代城市中扮演着越来越重要的角色,快速、准确、高效的指挥决策和调度是城市急救系统实现的关键.系统从功能和技术两方面入手,在分析整合平顶山市现有120急救系统的基础上引入GIS、GPS和GSM技术,利用组件mapobjects,结合visual basic开发语言,实现对数据的空间分析和管理,建立了基于GIS的城市120急救系统,实现了信息的查询分析、最佳路径分析以及缓冲区分析功能.     

Kinetic study on the reaction between CO2-CO and wustite using the isotope exchange method

To investigate the reaction between CO₂-CO and wustite using the isotope exchange method at 1073, 1173, 1273, and 1373 K, the experiment apparatus was designed to simulate the fluidized bed. The chemical rate constant was estimated by considering the effect of gas phase mass transfer on the reaction. It is found that the chemical rate constant is inversely decreased with the increase in the ratio of CO₂/CO by volume. The activation energy of reaction is in a linear relationship with the ratio of CO₂/CO by volume, and the average activation energy is 155.37 kJ/mol.     

Testing for the usefulness of forecasts

Ashley (Journal of Forecasting 1983; 2 (3): 211–223) proposes a criterion (known as Ashley's index) to judge whether the external macroeconomic variables are well forecast to serve as explanatory variables in forecasting models, which is crucial for policy makers. In this article, we try to extend Ashley's work by providing three testing procedures, including a ratio‐based test, a difference‐based test, and the Bayesian approach. The Bayesian approach has the advantage of allowing the flexibility of adapting all possible information content within a decision‐making environment such as the change of variable's definition due to the evolving system of national accounts. We demonstrate the proposed methods by applying six macroeconomic forecasts in the Survey of Professional Forecasters. Researchers or practitioners can thus formally test whether the external information is helpful. Copyright © 2010 John Wiley & Sons, Ltd.     

数据挖掘在网络教学评价中的应用

随着互联网和教育事业的日益融合和发展,网络教学已成为当今教育研究热点.通过关联规则对学生的教学反馈信息进行挖掘,寻找网络教学过程中有价值的信息,从而达到提高教学质量的效果.     

金属快速凝固理论及材料制备方法

快速凝固技术是近年来得到广泛发展和应用的新型材料的制备技术,其特点是具有较高的冷却速率和明显的非平衡效应,文章讨论了金属非平衡凝固过程的原理与方法,并对非平衡凝固技术在制备新材料中的应用作详细介绍.     

牙科畏惧症发病因素的初步探讨

1988年对920名大学生进行了牙科畏惧症发病因素的流行病学研究,同时采用Corah’s 问卷(CDAS)测量了525人的牙科畏惧水平,结果显示我国大学生的牙科畏惧水平高于国外同类报导。女性的 DAS 均值比男性高,差别有显著性(P<0.001)。随着近20年来我国龋齿发病率的上升,儿童时期的创伤性牙科经历相应增加。因此,这种直接的创伤性经历,牙科环境的影响,来自于家庭或同龄人对牙科的间接经验,以及居民对口腔保健的认识等,是牙科畏惧症的多种发病因素。     

FORTRAN语言打印机绘图软件及其应用

本文针对许多科技工作者应用计算机进行科学计算后,缺乏专门的绘图设备将数据绘成图形这一普遍存在的问题,用FORTRAH语言研制了点阵打印机绘图的程序。不需要增加任何硬件,便可很方便地绘制出各种设备图、函数曲线图以及标量和矢量场参数的分布图,使研究结果的输出形式更为形象、直观和清晰。