遗传学立体化教材的建设与研究

为适应现代遗传学的学科发展,促进高校生物创新人才的培养,变革高校传统的遗传学教材体系和教学模式已势在必行.在探究教学环节的过程中,针对目前高校遗传学教学中存在的普遍性问题,提出建设遗传学立体化教材,实施立体化的教学模式.本文主要从遗传学主教材的构建、多媒体课件的制作、学习指导书的编订以及试题库的建设等方面详细探讨这一新的教材体系的建设模式及其初步实践效果.     

网格化工商业市场监管系统设计与实现

介绍网格化工商市场监管系统的设计,采用分布式数据库结构,C/S与B/S相结合,实现了对企业监管的网格化、分级式管理,包括企业注册登记、企业信用等级监管、用户投诉与审核、企业荣誉与处罚等功能.     

Discovery and significance of buried paleoearthquake of the early Shang Dynasty (1260–1520 BC) in Xuecun, Xingyang, Henan Province, China

Due to the South-to-North Water Diversion Project,the salvage archaeological excavations were conducted in Xuecun,Xingyang city,Henan Province,China,by the Institute of Cultural Relics and Archaeology of Henan Province in 2005 and 2006.Ample evidence for a paleoearthquake was found during the excavation in the Xuecun archaeological site.The evidence includes faults,two small grabens and a series of ground fissures.These geological structures are considered to be generated by the earthquake.Based on the rela...     

Quantitative reactivity profiling predicts functional cysteines in proteomes

Cysteine is the most intrinsically nucleophilic amino acid in proteins, where its reactivity is tuned to perform diverse biochemical functions. The absence of a consensus sequence that defines functional cysteines in proteins has hindered their discovery and characterization. Here we describe a proteomics method to profile quantitatively the intrinsic reactivity of cysteine residues en masse directly in native biological systems. Hyper-reactivity was a rare feature among cysteines and it was found to specify a wide range of activities, including nucleophilic and reductive catalysis and sites of oxidative modification. Hyper-reactive cysteines were identified in several proteins of uncharacterized function, including a residue conserved across eukaryotic phylogeny that we show is required for yeast viability and is involved in iron-sulphur protein biogenesis. We also demonstrate that quantitative reactivity profiling can form the basis for screening and functional assignment of cysteines in computationally designed proteins, where it discriminated catalytically active from inactive cysteine hydrolase designs.     

混凝土中结合氯离子研究综述

混凝土中氯离子的结合会影响其侵蚀进程。且结合氯离子并不稳定,在酸化、碳化、化学侵蚀与通电作用等情况下会失稳释放,从水泥结合氯离子的机理、结合能力及结合氯离子稳定性等方面对中外研究现状进行综述。提出了对于某些因素需要建立更合理的评价标准,需要深入研究多因素耦合作用及降低失稳氯离子对钢筋腐蚀危害的研究建议。     

Effects of thermomechanical treatments on the microstructures and mechanical properties of GTA-welded AZ31B magnesium alloy

Thermomechanical treatments were carried out to improve the properties of AZ31B joints prepared by gas tungsten arc welding. The microstructures of the joints were studied by optical microscopy and scanning electron microscopy with energy-dispersive spectrometry. Tensile tests and hardness tests were performed to investigate the effects of thermomechanical treatments on the mechanical properties of the joints. It is found that the thermomechanical-treated joints show superior mechanical properties against the as-welded joints, and their ultimate tensile strength can reach more than 92% of the base material. This mainly attributes to the formation of fine equiaxed grains in the fusion zone. After thermomechanical treatments the dendrites are transformed to fine spherical grains, and the dendritic segregation can be effectively eliminated.     

应用光学原理研究原油沥青的聚沉过程

利用Turbiscan Lab 稳定性分析仪研究了原油/甲苯混合体系的稳定性。将不同加量的沉淀剂应用于1：9 的原油/甲苯混合体系中,通过测量原油/甲苯的透射光和背散射光强度随时间的变化,可描述混合体系中沥青的聚集和沉淀过程,有助于分析原油不稳定性发生的机理,并定量分析了沥青聚沉过程中的颗粒粒径大小、粒径增大速率和颗粒沉降速率等。实验表明,随着沉淀剂的加量从80.0% 增加到90.9%,混合体系中沥青的聚集速率从192.216 μm/min 增加到5 332.120 μm/min,粒径从10.0 μm 增大为53.4 μm ,沉积速率从0.17 mm/min 增加到0.46 mm/min。     

巨厚砾岩下开采地表沉陷预计模型的改进

为了探究巨厚砾岩下开采的地表沉陷规律,结合关键层理论,通过数值模拟从垂直应力、塑性区、垂直位移3个方面分析覆岩内部稳定性及造成地表倾向下沉曲线偏态的原因,基于概率积分法,建立改进的地表沉陷预计模型。结果表明:关键层初次破断距为291 m,周期破断距为281 m;悬力臂是造成下沉曲线偏态的主要原因;相对于经典的概率积分沉陷预计模型,改进的地表沉陷预计模型相对误差减小114%。可见在巨厚砾岩层下开采地表沉陷预计中,改进的地表沉降预计模型具有较高的准确度和适用性。     

长三角地区常用遥感降水产品质量评估

长三角地区是中国经济最发达地区之一,选定较好的卫星降水产品是进行科学防灾减灾的重要环节.基于地面气象站降水实测数据,采用7种指标对目前中外常用遥感降水产品热带降水任务卫星(tropical rainfall measurement mission,TRMM)、气候预测中心校正产品(climate prediction center morphing technique,CMORPH)、全球降水计划数据集(global precipi-tation measurement,GPM)以及中国自动站与CMORPH降水产品融合数据集(China merged precipitation analysis,CMPA)在长三角地区的适用性进行评估.结果表明:在年尺度,CMPA产品质量最优,相关系数达到0.95;在季节尺度,冬季GPM最优,夏季TRMM最优;平原地区比山区地区的产品质量要好.融合多降水产品的高精度产品未来将成为科学防灾减灾的重要数据.     

甘蓝型油菜BnHY5基因的表达模式及非生物胁迫响应分析

为探究甘蓝型油菜中HY5的表达情况,首先通过甘蓝型油菜基因组数据分析预测HY5及其同源基因HYH(HY5-Homologous),克隆获得了四个HY5(命名为BnHY5)以及三个HYH(命名为BnHYH)重复基因全长cDNA序列.其次,对BnHY5进行了蛋白结构预测、理化性质分析,研究结果表明:BnHY5蛋白为不稳定、亲水性蛋白,主要结构域为亮氨酸拉链.最后,探究了甘蓝型油菜中该基因的表达情况,用荧光定量PCR分析了BnHY5重复基因表达模式和响应非生物胁迫的情况,BnHY5四个基因中,BnHY5-2的表达水平最高,BnHY5-1、BnHY5-2不存在组织表达差异且表达水平高于BnHY5-3、BnHY5-4.BnHY5基因不仅响应高温、低温、镉、高盐非生物胁迫,而且参与ABA和GA_3激素信号响应,说明BnHY5在逆境胁迫中有重要作用.此外BnHY5重复基因在非生物胁迫下的表达模式发生改变,BnHY5四个基因的表达占比与不同类型胁迫处理相关,存在表达差异.