Expression, purification of a synthetic fuse-protein-TSF from PF4 and TSP1 fragments and its effect on angiogenesis and tumor growth

Sequences encoding PF4 (58–70) and TSP1 (429–459) were linked to yield a single gene TSF which encodes the fuse-protein of TSF. The gene was cloned into a pGEX-2T expression vector to generate a protein GST-TSF, which was strongly expressed inE. coli. The purified GST-TSF was degraded with thrombin to generate the protein TSF. With the methods of MTT and wound repair assay, the effects of TSF on the proliferation and migration of EC were detected, respectively. The results showed that TSF significantly suppressed BAEC proliferation and migration in a dose-dependent manner. The fuse protein GST-TSF, and the peptides PF4 (58–70) and TSP1 (429–459) also inhibited BAEC proliferation and migration, respectively, but their inhibition rates were not as high as TSF. Using the CAM assay, it was shown that TSF, GST-TSF, PF4 (58–70) and TSP1 (429–459) inhibited angiogenesis in chick CAM potentially, the effect of TSF was the highest.In vivo, the growth of Lewis lung carcinoma was potently inhibited by TSF treatment, and the inhibition rate was 68.75% at a dose of 1.00 μmol/kg · d. These findings suggest that the design on TSF gene was successful, and TSF with its anti-angiogenic and anti-tumor activity, should be a useful source of the inhibitor.     

自由基降解甲基橙溶液试验

针对含有偶氮类难降解有机物的染料废水具有色度大,生物可降解性低,难于处理等特点,模拟了有典型偶氮结构的甲基橙染料废水,对强电离放电产生的自由基溶液降解甲基橙进行了研究,讨论了pH值、甲基橙溶液初始质量浓度、自由基溶液质量浓度、温度以及反应时间等因素对甲基橙溶液降解性能的影响.结果表明,酸性条件有利于甲基橙溶液的降解,pH=1.5时,自由基氧化降解200 mg/L的甲基橙溶液3 min后,其COD的去除率和脱色率最大值分别达到86.87%和88.53%.对比甲基橙溶液处理前后紫外-可见光谱图的变化,发现经自由基降解后,偶氮键及苯环都受到破坏,大部分甲基橙分子已经氧化分解.     

微双折射光程差测量技术

研究了微双折射光程差测量技术，提出用旋转半波片－反射法测量半封闭玻璃制品应力，探讨以电光调制法为基础的微双折射光程差测量系统的稳定性。从软件和硬件两个方面建立起自动测量系统。实践证明达到了预期要求。     

温度对正负离子表面活性剂及其复配体系表面活性的影响

本文应用张力法测定了正负离子表面活性剂及其复配体系在不同温度下的表面张力，研究了各种体系的表面性质与温度的关系，并计算了在不同温度下的标准自由能变化．对不同体系的变化规律作了讨论．     

STM隔振系统的复模态分析

用复模态分析法求出了针对扫描隧道显微镜隔振系统的运动解和振幅传递函数的解析表达式，从该表达式可以看出，隔振系统第一级参数极大地影响着整个系统的隔振性能，然后用由本方法计算得出的三维和二维图象分析ＳＴＭ隔振系统，在悬挂弹簧加磁阻尼系统和金属块中夹橡圈系统的比较中，得出了一些有用的结论。     

总熵判据的作用和局限

首先讨论了环境熵变和热源熵变间的区别，以及两者相同时的条件；然后阐述了总熵判据和克劳修斯不等式在本质上并无不同之处，都可以用于判别过程的可逆性；最后将总熵变与隔离体系的熵变相比较，对它们的用途进行了详细讨论。     

低能N+注入凤仙花的变异检测

以低能N 注入凤仙花(Impatiens balsaminaL)种子后获得了高茎、矮茎2个变异品系.对其进行过氧化物同工酶(POD)检测的结果表明,2个变异品系叶片的POD分子大小、表达强度和条带数目与对照相比都发生了一定变化.随机扩增多态性DNA(random amplified polymorphic DNA,RAPD)分析结果也表明变异品系DNA链的脱氧核苷酸序列发生了变异,从25个随机引物中筛选出了9个具有多态性.实验证明适当剂量的低能N 注入能够在基因水平引起凤仙花的变异,为花卉产业的发展提供了一种遗传育种手段.     

Hg1—xCdxTe液相外延薄膜的貌相分析

用扫描电子显微镜、能量散射Ｘ－射线分析及金相显微镜等方法，研究了Ｈｇ１－ｘＣｄｘＴｅ液相外延薄膜的表面形貌与衬底沾污等的关系，以及外延层夹杂和晶界存在的影响。结果表明，衬底沾污及晶界的存在会使外延薄膜的貌相变差；生长前衬底表面覆盖可以减轻其表面沾污，用适当的回熔ＬＰＥ工艺，可以改进外延层表面形貌及质量