Production and characterization of antibody against aflatoxin M1

Antibody against aflatoxin M1 was obtained after immunization of rabbits with bovine serum albumin-afla M1 oxime conjugate. The antibody has greatest binding efficiency for afla M1, and was less efficient for afla B1. Cross-reaction of antibody with aflatoxin Q1, aflatoxicol, and aflatoxin B2a was weak. Aflatoxin B2, G1, and G2 and afla B1-guanine adducts showed almost no cross-reaction with the antibody. The sensitivity of the binding assay for aflatoxin M1 detection is in the range of 1-10 ng per assay. Detailed methods for the preparation of the conjugate, production of immune serum, and methods for antibody determination are described.     

Quantitative reactivity profiling predicts functional cysteines in proteomes

Cysteine is the most intrinsically nucleophilic amino acid in proteins, where its reactivity is tuned to perform diverse biochemical functions. The absence of a consensus sequence that defines functional cysteines in proteins has hindered their discovery and characterization. Here we describe a proteomics method to profile quantitatively the intrinsic reactivity of cysteine residues en masse directly in native biological systems. Hyper-reactivity was a rare feature among cysteines and it was found to specify a wide range of activities, including nucleophilic and reductive catalysis and sites of oxidative modification. Hyper-reactive cysteines were identified in several proteins of uncharacterized function, including a residue conserved across eukaryotic phylogeny that we show is required for yeast viability and is involved in iron-sulphur protein biogenesis. We also demonstrate that quantitative reactivity profiling can form the basis for screening and functional assignment of cysteines in computationally designed proteins, where it discriminated catalytically active from inactive cysteine hydrolase designs.     

地震荷载作用下核级(IE)蓄电池电气性能研究

简要介绍核级蓄电池模拟地震试验的试件选择、安装环境的模拟以及试验的基本程序,重点讨论地震荷载作用期间蓄电池连续放电特性,以及试验前后蓄电池容量的考核等特性问题。验证了样机设计的可靠性及合理性,为改进设计及现场安装提供了依据。还论证了国产蓄电池应用在核电领域中的可行性。     

富锗煤烟灰中的锗含量测定

建立了湿法消解-电感耦合等离子发射光谱法(ICP-AES)测定富锗煤烟灰中锗含量的方法,研究了样品预处理、进样系统、光谱干扰、等离子体测定条件对测定结果的影响.在优化实验条件下,方法的相对标准偏差0.79%,加标回收率98.55%~105.85%,表明该方法具有较好的准确度和精密度.     

汽车ABS电机PWM控制方法研究

通过对ABS工作过程中制动液流量的分析,确定了ABS电机的基本工作要求.结合ABS电机的工作特性,采用基于逻辑门限值的PWM控制方法控制电机.运用DSpace搭建快速原型试验台,对比在不同电机工作转速、PWM控制频率下的噪声和制动踏板感觉,优化逻辑门限值大小,得出合理的工作方式,使电机既能满足控制要求,又有较长的寿命.通过在不同路面的试验结果对该控制方法的两个关键参数进行了优化.最后进行不同工况的试验,结果表明:通过优化控制参数的PWM控制方法,具有较强的鲁棒性,能够满足ABS在任何工作条件下对电机的要求.     

在物化实验中用自编程序实现计算机数据处理

在我们所开设的物理化学实验中有许多电化学实验,用扫描恒电位仪发出信号,Ｘ－Ｙ记录仪接收信号,因为普通扫描情怀电位仪没有计算机接口,所以不能用计算机采集数据及处理。本文通过采用信号处理仪代替Ｘ－Ｙ记录仪采用数据并通过自编软件帝现数据转换,再用美国ＥＧ＆Ｇ－ＰＡＲ公司提供的电化学软件包解析数据,实现数据采集及处理的计算机化,减小了数据记录及处理的误差,求得了相应的电化学参数。     

Evaluation of humic acid removal by a flat submerged membrane photoreactor

A flat submerged membrane combined with a TiO 2 /UV photocatalytic reactor (FSMPR) was employed in batch mode to remove humic acid (HA). HA removal efficiency was characterized by UV 254 absorbance, UV-vis spectra, dissolved organic carbon (DOC) concentration, specific UV absorbance (SUVA), and trihalomethane formation potential (THMFP). The FSMPR process was effective in removing more than 86% of DOC and nearly 100% of UV 254 absorbance, while the THMFPs of samples were reduced to < 19 μ g/L after 150 min of treatment. In addition, changes in transmembrane pressure (TMP) with and without UV were evaluated; TiO 2 /UV was effective at controlling membrane fouling by HA. Analysis of the molecular weight (MW) distributions and three-dimensional excitation-emission matrix (EEM) fluorescence spectra of HAs revealed that the effectiveness in membrane fouling control is a result of changes in HA molecular characteristics. The TiO 2 /UV photocatalytic reactor caused the degradation of high MW, hydrophobic humic-like molecules to low MW, hydrophilic protein-like molecules, although this fraction was not completely removed during 150 min of treatment and was less responsible for membrane fouling.     

一个非线性系统动力学行为的注记

讨论参数λ＜０时非线性系统ｘｎ＋１＝λｘｎ（１－ｘｎ）的动力学行为．     

好稿外流外稿选优

“好稿外流”造成学报稿原匮乏的情况，在市属高校不同程度地存在着；与此同时，“外稿”却与日俱增，补充了学报的文稿，也带来了用稿的矛盾。解决这个矛盾的正确办法是既不反对好稿外流，又要做了外稿选优的工作，以保证学报的稿源和质量