Habitat-induced reciprocal transformation in the root phenotype of Oriental ginseng is associated with alteration in DNA methylation

Oriental ginseng is an important medicinal plant that grows in 2 major forms or ecotypes, wild and domesticated. Each form differs conspicuously in root phenotype, but can be converted from one type to another by habitat. Here we show that the habitat-induced transformation of ginseng root phenotype was accompanied by alteration in cytosine methylation at a large number of 5′-CCGG-3′ sites detected by the methylation-sensitive polymorphism (MSAP) marker. The collective CG and CHG methylation levels of all 4 landraces of the domesticated form were significantly lower than those of the wild form. Interestingly, artificially transplanted ginseng plants recreated in both directions the methylation levels (at least in CHG) of their natural counterparts. The methylation differences between the 2 ginseng ecotypes were validated at 2 isolated MSAP loci bearing homology to a 5S rRNA gene or a copia retrotransposon. Our results implicate a link between epigenetic variation and habitat-induced phenotypic flexibility in Oriental ginseng.     

Evaluation of humic acid removal by a flat submerged membrane photoreactor

A flat submerged membrane combined with a TiO 2 /UV photocatalytic reactor (FSMPR) was employed in batch mode to remove humic acid (HA). HA removal efficiency was characterized by UV 254 absorbance, UV-vis spectra, dissolved organic carbon (DOC) concentration, specific UV absorbance (SUVA), and trihalomethane formation potential (THMFP). The FSMPR process was effective in removing more than 86% of DOC and nearly 100% of UV 254 absorbance, while the THMFPs of samples were reduced to < 19 μ g/L after 150 min of treatment. In addition, changes in transmembrane pressure (TMP) with and without UV were evaluated; TiO 2 /UV was effective at controlling membrane fouling by HA. Analysis of the molecular weight (MW) distributions and three-dimensional excitation-emission matrix (EEM) fluorescence spectra of HAs revealed that the effectiveness in membrane fouling control is a result of changes in HA molecular characteristics. The TiO 2 /UV photocatalytic reactor caused the degradation of high MW, hydrophobic humic-like molecules to low MW, hydrophilic protein-like molecules, although this fraction was not completely removed during 150 min of treatment and was less responsible for membrane fouling.     

基于RFID技术的电子围栏内施工人员行为预判与告警算法

在电子围栏内施工人员行为预判与告警研究中,面对不确定的行为信息,常见的告警算法存在大量时隙分配冲突,导致算法的自适应性较差。为此,提出基于射频识别(Radio Frequency Identification, RFID)技术的电子围栏内施工人员行为预判与告警算法。通过远程通信和RFID读写器实时读取施工人员的位置信息和行为信息,将逻辑运算符与时间约束运算符相结合预判施工人员行为,在预判到危险行为后,产生相应的告警信息,设计防冲突算法,将告警信息传递到对应的电子标签上,实现对施工人员的告警。实验结果表明：面对不确定的行为信息,设计的告警算法对施工人员行为信息识别水平高,在时隙分配中未发生冲突事件,且分配成功率在99%以上,其自适应性得到提升。     

A20 (TNFAIP3) deficiency in myeloid cells triggers erosive polyarthritis resembling rheumatoid arthritis

A20 (TNFAIP3) is a protein that is involved in the negative feedback regulation of NF-κB signaling in response to specific proinflammatory stimuli in different cell types and has been suggested as a susceptibility gene for rheumatoid arthritis. To define the contribution of A20 to rheumatoid arthritis pathology, we generated myeloid-specific A20-deficient mice and show that specific ablation of Tnfaip3 in myeloid cells results in spontaneous development of a severe destructive polyarthritis with many features of rheumatoid arthritis. Myeloid-A20-deficient mice have high levels of inflammatory cytokines in their serum, consistent with a sustained NF-κB activation and higher TNF production by macrophages. Destructive polyarthritis in myeloid A20 knockout mice was TLR4-MyD88 and IL-6 dependent but was TNF independent. Myeloid A20 deficiency also promoted osteoclastogenesis in mice. Together, these observations indicate a critical and cell-specific function for A20 in the etiology of rheumatoid arthritis, supporting the idea of developing A20 modulatory drugs as cell-targeted therapies.     

Discrete Euler processes and their applications

This paper introduces discrete Euler processes and shows their application in detecting and forecasting cycles in non‐stationary data where periodic behavior changes approximately linearly in time. A discrete Euler process becomes a classical stationary process if ‘time’ is transformed properly. By moving from one time domain to another, one may deform certain time‐varying data to non‐time‐varying data. With these non‐time‐varying data on the deformed timescale, one may use traditional tools to do parameter estimation and forecasts. The obtained results then can be transformed back to the original timescale. For datasets with an underlying discrete Euler process, the sample M‐spectrum and the spectra estimator of a Euler model (i.e., EAR spectral) are used to detect cycles of a Euler process. Beam response and whale data are used to demonstrate the usefulness of a Euler model. Copyright © 2008 John Wiley & Sons, Ltd.     

基于C/S结构的城市公交监控系统设计

介绍了一种基于C/S结构的城市公交车监控系统,阐述了在Delphi编程环境下如何利用socket编程机制实现客户端与服务器的数据交换,实现了城市公交监控系统的设计。     

光学活性4(S)-2-甲基-4-甲氧羰基-2-噁唑啉的合成和开环聚合

以L—丝氨酸为原料,合成了旋光纯度很高的4(S)—2—甲基—4—甲氧羰基—2—噁唑啉单体。该化合物在对甲基苯磺酸、硫酸二甲酯等引发剂作用下进行正离子开环聚合,获得了具有光学活性的聚合物。结构分析表明,产物具有两种类型的结构,反应温度以及引发剂用量对这两种结构成份的相对含量有影响。对开环聚合机理进行了初步探讨。     

不同培养条件对朱顶红形态发生的影响

作者通过试验探讨了促进朱顶红快速成苗的有效途径。     

鹿茸的组织结构及离体细胞的显微结构观察

本文对鹿茸的组织结构、鹿茸皮肤及顶端肉质部分的显微结构进行了较详尽的观察,同时对从鹿茸分离的细胞及由鹿茸组培养出的细胞形态结构也作了初步研究。     

滞后对象的连续模型参考自适应控制

本文基于MRAC的基本原理,提出了一种从模型取状态的滞后系统的MRAC方案。这种方法较之其它方案结构简单,且具有一定的抗输出端扰动的能力。仿真结果说明了系统结构是可行的。