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排序方式: 共有164条查询结果,搜索用时 31 毫秒
111.
INTRODUCTIONFungal laccases (benzenediol:oxygen oxidoreductases, EC 1.10.3.2) are multicopper oxidases, capable of reducing oxygen to water and simultaneously involved in oxidation of aromatic hydrogen donors. These enzymes and very similar polyphenol oxidases can be used as free or immobilized type both in water or in some organic solvents for improving several biotechnological processes (Burton etal. 1995; Luterek etal. 1998; Milstein etal. 1993). Of possible applications, the enzyme i… 相似文献
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一类高阶非线性系统的零解稳定性 总被引:1,自引:0,他引:1
由Барбащин公式得到的四阶常系数线性系统的Ляпунов函数出发,通过类比法构造了一类四阶非线性系统的Ляпунов函数,并由此得到了这些系统零解的全局渐近稳定性的充分条件 相似文献
115.
In Ha Cho Min Jung Lee Dae Hwan Kim Bora Kim Jeomil Bae Kyu Yeong Choi Seon-Myung Kim Yun Hyun Huh Kun Ho Lee Chong-Hyun Kim Woo Keun Song 《Cellular and molecular life sciences : CMLS》2013,70(22):4369-4383
Actin plays a fundamental role in the regulation of spine morphology (both shrinkage and enlargement) upon synaptic activation. In particular, actin depolymerization is crucial for the spine shrinkage in NMDAR-mediated synaptic depression. Here, we define the role of SPIN90 phosphorylation/dephosphorylation in regulating actin depolymerization via modulation of cofilin activity. When neurons were treated with NMDA, SPIN90 was dephosphorylated by STEP61 (striatal-enriched protein tyrosine phosphatase) and translocated from the spines to the dendritic shafts. In addition, phosphorylated SPIN90 bound cofilin and then inhibited cofilin activity, suggesting that SPIN90 dephosphorylation is a prerequisite step for releasing cofilin so that cofilin can adequately sever actin filaments into monomeric form. We found that SPIN90 YE, a phosphomimetic mutant, remained in the spines after NMDAR activation where it bound cofilin, thereby effectively preventing actin depolymerization. This led to inhibition of the activity-dependent redistribution of cortactin and drebrin A, as well as of the morphological changes in the spines that underlie synaptic plasticity. These findings indicate that NMDA-induced SPIN90 dephosphorylation and translocation initiates cofilin-mediated actin dynamics and spine shrinkage within dendritic spines, thereby modulating synaptic activity. 相似文献
116.
Optic atrophy 3 as a protein of the mitochondrial outer membrane induces mitochondrial fragmentation
Seung-Wook Ryu Hyeon Joo Jeong Myunghwan Choi Mariusz Karbowski Chulhee Choi 《Cellular and molecular life sciences : CMLS》2010,67(16):2839-2850
The optic atrophy 3 (OPA3) gene, which has no known homolog or biological function, is mutated in patients with hereditary
optic neuropathies. Here, we identified OPA3 as an integral protein of the mitochondrial outer membrane (MOM), with a C-terminus
exposed to the cytosol and an N-terminal mitochondrial targeting domain. By quantitative analysis, we demonstrated that overexpression
of OPA3 significantly induced mitochondrial fragmentation, whereas OPA3 knockdown resulted in highly elongated mitochondria.
Cells with mitochondria fragmented by OPA3 did not undergo spontaneous apoptotic cell death, but were significantly sensitized
to staurosporine- and TRAIL-induced apoptosis. In contrast, overexpression of a familial OPA3 mutant (G93S) induced mitochondrial
fragmentation and spontaneous apoptosis, suggesting that OPA3 mutations may cause optic atrophy via a gain-of-function mechanism.
Together, these results indicate that OPA3, as an integral MOM protein, has a crucial role in mitochondrial fission, and provides
a direct link between mitochondrial morphology and optic atrophy. 相似文献
117.
Differential use of an in-frame translation initiation codon regulates human mu opioid receptor (OPRM1) 总被引:1,自引:1,他引:0
Kyu Young Song Hack Sun Choi Cheol Kyu Hwang Chun Sung Kim Ping-Yee Law Li-Na Wei Horace H. Loh 《Cellular and molecular life sciences : CMLS》2009,66(17):2933-2942
The pharmacological effects of morphine and morphine-like drugs are mediated primarily through the μ opioid receptor. Here
we show that differential use of an in-frame translational start codon in the 5′-untranslated region of the OPRM1 generates
different translational products in vivo and in vitro. The 5′-end of the OPRM1 gene is necessary for initiating the alternate
form and for subsequent degradation of the protein. Initiation of OPRM1 at the upstream site decreases the initiation at the
main AUG site. However, alternative initiation of the long form of OPRM1 produces a protein with a short half-life, resulting
from degradation mediated by the ubiquitin–proteasome pathway. Reporter and degradation assays showed that mutations of this
long form at the second and third lysines reduce ubiquitin-dependent proteasome degradation, stabilizing the protein. The
data suggest that MOP expression is controlled in part by initiation of the long form of MOP at the alternate site. 相似文献
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Distinct molecular mechanism for initiating TRAF6 signalling 总被引:20,自引:0,他引:20
Ye H Arron JR Lamothe B Cirilli M Kobayashi T Shevde NK Segal D Dzivenu OK Vologodskaia M Yim M Du K Singh S Pike JW Darnay BG Choi Y Wu H 《Nature》2002,418(6896):443-447
Tumour-necrosis factor (TNF) receptor-associated factor 6 (TRAF6) is the only TRAF family member that participates in signal transduction of both the TNF receptor (TNFR) superfamily and the interleukin-1 receptor (IL-1R)/Toll-like receptor (TLR) superfamily; it is important for adaptive immunity, innate immunity and bone homeostasis. Here we report crystal structures of TRAF6, alone and in complex with TRAF6-binding peptides from CD40 and TRANCE-R (also known as RANK), members of the TNFR superfamily, to gain insight into the mechanism by which TRAF6 mediates several signalling cascades. A 40 degrees difference in the directions of the bound peptides in TRAF6 and TRAF2 shows that there are marked structural differences between receptor recognition by TRAF6 and other TRAFs. The structural determinant of the petide TRAF6 interaction reveals a Pro-X-Glu-X-X-(aromatic/acidic residue) TRAF6-binding motif, which is present not only in CD40 and TRANCE-R but also in the three IRAK adapter kinases for IL-1R/TLR signalling. Cell-permeable peptides with the TRAF6-binding motif inhibit TRAF6 signalling, which indicates their potential as therapeutic modulators. Our studies identify a universal mechanism by which TRAF6 regulates several signalling cascades in adaptive immunity, innate immunity and bone homeostasis. 相似文献
120.
Y. Choi 《Cellular and molecular life sciences : CMLS》1985,41(1):127-129
Summary The effect of 750 second chromosomes ofDrosophila melanogaster on viability was studied. 19.3% of them proved letal or semilethal (=drastics) in homozygous condition. Compared to data obtained in previous years at the same sampling site, a significant frequency decrease of drastics during the past decade could be observed. The dynamic processes taking place in the Korean wild populations ofD. melanogaster are discussed.Acknowledgment. This work was supported by a research grant from the Korean Science and Engineering Foundation. 相似文献