An exonucleolytic activity of human apurinic/apyrimidinic endonuclease on 3' mispaired DNA

Human apurinic/apyrimidinic endonuclease (APE1) is an essential enzyme in DNA base excision repair that cuts the DNA backbone immediately adjacent to the 5' side of abasic sites to facilitate repair synthesis by DNA polymerase beta (ref. 1). Mice lacking the murine homologue of APE1 die at an early embryonic stage. Here we report that APE1 has a DNA exonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules. The efficiency of this activity is inversely proportional to the gap size in DNA. In a base excision repair system reconstituted in vitro, the rejoining of nicked mismatched DNA depended on the presence of APE1, indicating that APE1 may increase the fidelity of base excision repair and may represent a new 3' mispaired DNA repair mechanism. The exonuclease activity of APE1 can remove the anti-HIV nucleoside analogues 3'-azido-3'-deoxythymidine and 2',3'-didehydro-2', 3'-dideoxythymidine from DNA, suggesting that APE1 might have an impact on the therapeutic index of antiviral compounds in this category.     

"三个代表"是中国"实践的唯物主义者"的使命

"实践的唯物主义者,即共产主义者","三个代表"的重要思想是对马、恩有关共产主义者使命著名论断的有力坚持和发展,充分体现了共产主义者使命的共性与个性的内在统一,渗透和贯穿了"实践的唯物主义"的丰富而深刻的哲学内涵.共产主义者使命的哲学依据,是基于实践辩证地把握和对待现存世界、社会历史及人生的"实践的唯物主义".     

一个非线性电路模型的混沌态特性分析

对非线性系统进行分析时,可能出现测量结果为混沌态,以一个非线性电路模型为例。分析混沌态的几率密度并提出“赝温度”的新概念,以表征混沌态的赝随机变化的剧烈程度。从而为非线性系统的混沌态进行“乱中有序”的定量描述提供了一种新方法。     

Gigantic jets between a thundercloud and the ionosphere

Transient luminous events in the atmosphere, such as lighting-induced sprites and upwardly discharging blue jets, were discovered recently in the region between thunderclouds and the ionosphere. In the conventional picture, the main components of Earth's global electric circuit include thunderstorms, the conducting ionosphere, the downward fair-weather currents and the conducting Earth. Thunderstorms serve as one of the generators that drive current upward from cloud tops to the ionosphere, where the electric potential is hundreds of kilovolts higher than Earth's surface. It has not been clear, however, whether all the important components of the global circuit have even been identified. Here we report observations of five gigantic jets that establish a direct link between a thundercloud (altitude approximately 16 km) and the ionosphere at 90 km elevation. Extremely-low-frequency radio waves in four events were detected, while no cloud-to-ground lightning was observed to trigger these events. Our result indicates that the extremely-low-frequency waves were generated by negative cloud-to-ionosphere discharges, which would reduce the electrical potential between ionosphere and ground. Therefore, the conventional picture of the global electric circuit needs to be modified to include the contributions of gigantic jets and possibly sprites.     

Adaptive terminal sliding mode control for high-order nonlinear dynamic systems

An adaptive terminal sliding mode control (SMC) technique is proposed to deal with the tracking problem for a class of high-order nonlinear dynamic systems. It is shown that a function augmented sliding hyperplane can be used to develop a new terminal sliding mode for high-order nonlinear systems. A terminal SMC controller based on Lyapunov theory is designed to force the state variables of the closed-loop system to reach and remain on the terminal sliding mode, so that the output tracking error then converges to zero in finite time which can be set arbitrarily. An adaptive mechanism is introduced to estimate the unknown parameters of the upper bounds of system uncertainties. The estimates are then used as controller parameters so that the effects of uncertain dynamics can be eliminated. It is also shown that the stability of the closed-loop system can be guaranteed with the proposed control strategy. The simulation of a numerical example is provided to show the effectiveness of the new method.     

Axisymmetric fundamental solutions for a finite layer with impeded boundaries

Axisymmetrie fundamental solutions that are applied in the consolidation calculations of a finite clay layer with impeded boundaries were derived. Laplace and Hankel integral transforms were utilized with respect to time and radial coordinates, respectively in the analysis. The derivation of fundamental solutions considers two boundary-value problems involving unit point loading and ring loading in the vertical. The solut-ions are extended to circular distributed and strip distributed normal load. The computation and analysis of set-tlements, vertical total stress and excess pore pressure in the consolidation layer subject to circular loading are presented.     

实时数据通讯的方法及应用

介绍了在Windows98环境下,通过Modem和公用电话网(PSTN),利用设置好的"拨号网络",实现PC机系统之间的实时数据传输的方法。讨论了运用VB语言,调用WindowsAPI所提供的远程访问函数,设计了一个专用于"拨号网络"通讯的类,并详细介绍了该类的功能和用法。     

基于GA优化的城市可持续发展评价的普适公式

在分析城市可持续发展指标体系的特征基础上,分别确定了用遗传算法优化的持续、协调、结构和集聚４类单项指标的发展指数的普适公式;并对可持续发展的不同层次指标分别采用广义对比分析法和层次分析法进行加权,建立了城市可持续发展的综合指数评价模型。模型应用于乌鲁木齐的可持续发展评价结果与实况定性分析结果一致。该评价模型简单、实用,具有可比性和通用性。     

The mammalian sodium channel BNC1 is required for normal touch sensation

Of the vertebrate senses, touch is the least understood at the molecular level The ion channels that form the core of the mechanosensory complex and confer touch sensitivity remain unknown. However, the similarity of the brain sodium channel 1 (BNC1) to nematode proteins involved in mechanotransduction indicated that it might be a part of such a mechanosensor. Here we show that disrupting the mouse BNC1 gene markedly reduces the sensitivity of a specific component of mechanosensation: low-threshold rapidly adapting mechanoreceptors. In rodent hairy skin these mechanoreceptors are excited by hair movement. Consistent with this function, we found BNC1 in the lanceolate nerve endings that lie adjacent to and surround the hair follicle. Although BNC1 has been proposed to have a role in pH sensing, the acid-evoked current in cultured sensory neurons and the response of acid-stimulated nociceptors were normal in BNC1 null mice. These data identify the BNC1 channel as essential for the normal detection of light touch and indicate that BNC1 may be a central component of a mechanosensory complex.