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991.
The stripe disease-specific protein (SP) encoded by the rice stripe virus (RSV) was successfully used as a localization signal
of the virus in its vector, the small brown lanthopper,Laodelphax striatellus Fallen. Immunogold particles in large numbers were detected in various parts of the viruliferous females: the ovum, surface
of chorion, the midgut lumen, and the columnar cells. Whereas there was none of these particles in the non-viruliferous females
and males, and testis of viruliferous males. Endosymbionts (mycetocytes) were abundant, harboring ovaries of both viruliferous
and non-viruliferous females, but none in the testis of males. The results provided us with the direct proof that RSV is a
ciruculative and propagative plant virus and it was transovarially transmitted alongside with endosymbionts of its vector.
Therefore, we deem it a nice lead for future studies on the mechanism of RSV transmission and functioning of its viral proteins. 相似文献
992.
Shen Xianrong Ji Dongmei Hu Youqing Jia Fuxing Deng Xinxian Wang Ling Chu Zhiyong Jiang Dingwen Lei Chengxiang Huang Jiansheng Hu Xinran Ren Daming 《科学通报(英文版)》2001,46(24):2037-2042
Sequences encoding PF4 (58–70) and TSP1 (429–459) were linked to yield a single gene TSF which encodes the fuse-protein of TSF. The gene was cloned into a pGEX-2T expression vector to generate a protein GST-TSF, which was strongly expressed inE. coli. The purified GST-TSF was degraded with thrombin to generate the protein TSF. With the methods of MTT and wound repair assay, the effects of TSF on the proliferation and migration of EC were detected, respectively. The results showed that TSF significantly suppressed BAEC proliferation and migration in a dose-dependent manner. The fuse protein GST-TSF, and the peptides PF4 (58–70) and TSP1 (429–459) also inhibited BAEC proliferation and migration, respectively, but their inhibition rates were not as high as TSF. Using the CAM assay, it was shown that TSF, GST-TSF, PF4 (58–70) and TSP1 (429–459) inhibited angiogenesis in chick CAM potentially, the effect of TSF was the highest.In vivo, the growth of Lewis lung carcinoma was potently inhibited by TSF treatment, and the inhibition rate was 68.75% at a dose of 1.00 μmol/kg · d. These findings suggest that the design on TSF gene was successful, and TSF with its anti-angiogenic and anti-tumor activity, should be a useful source of the inhibitor. 相似文献
993.
The local distribution of preferred orientation and misorientation in TiN whiskers in TiNw-AINp/A1 composite system is investigated using backscattering electron diffraction combined with a scanning electron microscope. These preliminary results demonstrate the utility of this new automated technique for characterizing local textures in composite materials using backscattering electron diffraction. The composite investigated comprises TiN whiskers in a polycrystalline aluminum matrix. The local textures of samples before and after deformation were measured. The misorientation distributions show a breakdown of the monocrystal of TiN whisker in the deformed composite material relative to the starting material and an increase in small angle misorientations. 相似文献
994.
彩色等离子体显示器智能接口电路的研究 总被引:1,自引:0,他引:1
提出了可自动识别信号制试和格式的彩色等离子体显示(PDP)接口电路和实现方法,该电路可智能识别并适应常见不同分辨率的VGA信号格式和NTSC、PAL等两种电视制式,其音频处理部分支持最新的丽音标准,带有杜比Pro Logic处理功能。研究和实验结果发现,本接口电路应用于54cm和107cm彩色PDP的图像显示效果均很好,完全达到了实用化的程度。 相似文献
995.
Li1+2x +yAlxEuyTi2 -x -ySixP3 -xO12 锂快离子导体可用精选的天然高岭石Al4 [Si4 O10 ](OH) 8为原料 ,与Li2 CO3,TiO2 ,NH4 H2 PO4 ,Eu2 O3 高温 (80 0~ 10 0 0℃ )固相反应约 2 0h而制得 .在 y =0 .5 ,x≤ 0 .3 ;x =0 .2 ,y≤ 0 .3的原始组成范围内 ,可以形成一个空间群为R3C的固熔体相 .它们具有较好的离子电导率和较小的离子导电活化能 .当x =0 .2 ,y =0 .1,t=40 0℃时 ,其电导率达 9.98mS·cm-1,其活化能为 34.0kJ.mol-1. 相似文献
996.
邻硝基对甲基苯酚的相转移催化合成 总被引:2,自引:0,他引:2
研究了用季nin盐作相转移催化剂催化合成邻硝基对甲基苯酚,在18%的硝酸、1.0g催化剂、反应温度20℃~25℃、反应45min的条件下,获得70%以上的产率。此法具有产率高、易操作、反应条件温和、催化剂能重复使用的优点。 相似文献
997.
研究以像增强CCD为光电探测器的弱光Hartmann-Shack(H-S)波前传感器在质心同探测过程中阈值选取对质心探测精度的影响,首先给出了阈值选取对质心探测系统误差和随机误差的影响,一义质心测量精度的评价指标,并根据此指标指出阈值选取的最佳范围为-3σr≤Tm≤-σr,并以实验验证了理论分析结果。 相似文献
998.
煤的可溶化技术与煤的化学族组成 总被引:6,自引:0,他引:6
综述了煤可溶化技术研究的现状,讨论了影响煤可溶化的主要因素以及几种强化方法,指出了今后应作深入研究的几个方面,并提出了利用煤的可深化技术进行煤的化学族组成研究的设想。 相似文献
999.
理论研究与企业实践表明,激烈的市场竞争要求企业必须抛弃传统基于劳 动分工的职能管理思想,而采用以流程为基础的集成化管理的思想.为此,传统的生 产、营销等职能部门的决策应该得到有效的协调.本文在供应链背景下提出了一种生 产批量与定价的联合决策模型.在分析了最优联合决策的性质后,开发了求解模型的 算法,以有效地辅助供应链协调管理.,It is shown that the process-based management philosophy should be used to replace the la bor-division-based management philosophy so that the company can survive in the intensive competitive market. For this reason, the traditional department of production, marketing and finance should be coor dinated. In this paper, a joint decision model is proposed for the management of production decision and the marketing decision in a supply chain. In the model, the marketing variable is the quantity discount pricing policy and the production variable is the lot-sizing policy, which were studied separately in the past researches. After the properties of the solution to the model are studied, an effective algorithm is de veloped for supporting the supply chain management. 相似文献
1000.
地形特征匹配辅助导航方法研究 总被引:1,自引:0,他引:1
对传统的地形轮廓匹配辅助导航算法进行了如下改进:首先在景象匹配的基础上对飞行区域作出预测,其次利用实测高程度列和预测高程序列匹配,对飞行器进行实时定位和下一时刻的航迹预测;最后利用递堆和缩小匹配网格搜索范围等手段减少计算量。该方法提高了地形轮廓匹配的实时性和精度,增加了飞行器超低空飞行的可靠性和机动性。 相似文献