全文获取类型
收费全文 | 3721篇 |
免费 | 114篇 |
国内免费 | 179篇 |
专业分类
系统科学 | 151篇 |
丛书文集 | 110篇 |
教育与普及 | 135篇 |
理论与方法论 | 14篇 |
现状及发展 | 81篇 |
研究方法 | 44篇 |
综合类 | 3477篇 |
自然研究 | 2篇 |
出版年
2024年 | 19篇 |
2023年 | 39篇 |
2022年 | 64篇 |
2021年 | 75篇 |
2020年 | 59篇 |
2019年 | 26篇 |
2018年 | 42篇 |
2017年 | 51篇 |
2016年 | 56篇 |
2015年 | 95篇 |
2014年 | 127篇 |
2013年 | 146篇 |
2012年 | 176篇 |
2011年 | 178篇 |
2010年 | 181篇 |
2009年 | 169篇 |
2008年 | 193篇 |
2007年 | 143篇 |
2006年 | 135篇 |
2005年 | 120篇 |
2004年 | 85篇 |
2003年 | 161篇 |
2002年 | 272篇 |
2001年 | 257篇 |
2000年 | 181篇 |
1999年 | 148篇 |
1998年 | 80篇 |
1997年 | 91篇 |
1996年 | 74篇 |
1995年 | 65篇 |
1994年 | 79篇 |
1993年 | 52篇 |
1992年 | 53篇 |
1991年 | 60篇 |
1990年 | 53篇 |
1989年 | 60篇 |
1988年 | 48篇 |
1987年 | 18篇 |
1986年 | 11篇 |
1985年 | 9篇 |
1984年 | 4篇 |
1981年 | 4篇 |
1979年 | 6篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1972年 | 6篇 |
1971年 | 4篇 |
1968年 | 3篇 |
1966年 | 5篇 |
1957年 | 3篇 |
排序方式: 共有4014条查询结果,搜索用时 0 毫秒
141.
Structural insight into brassinosteroid perception by BRI1 总被引:1,自引:0,他引:1
She J Han Z Kim TW Wang J Cheng W Chang J Shi S Wang J Yang M Wang ZY Chai J 《Nature》2011,474(7352):472-476
Brassinosteroids are essential phytohormones that have crucial roles in plant growth and development. Perception of brassinosteroids requires an active complex of BRASSINOSTEROID-INSENSITIVE 1 (BRI1) and BRI1-ASSOCIATED KINASE 1 (BAK1). Recognized by the extracellular leucine-rich repeat (LRR) domain of BRI1, brassinosteroids induce a phosphorylation-mediated cascade to regulate gene expression. Here we present the crystal structures of BRI1(LRR) in free and brassinolide-bound forms. BRI1(LRR) exists as a monomer in crystals and solution independent of brassinolide. It comprises a helical solenoid structure that accommodates a separate insertion domain at its concave surface. Sandwiched between them, brassinolide binds to a hydrophobicity-dominating surface groove on BRI1(LRR). Brassinolide recognition by BRI1(LRR) is through an induced-fit mechanism involving stabilization of two interdomain loops that creates a pronounced non-polar surface groove for the hormone binding. Together, our results define the molecular mechanisms by which BRI1 recognizes brassinosteroids and provide insight into brassinosteroid-induced BRI1 activation. 相似文献
142.
Bivona TG Hieronymus H Parker J Chang K Taron M Rosell R Moonsamy P Dahlman K Miller VA Costa C Hannon G Sawyers CL 《Nature》2011,471(7339):523-526
Human lung adenocarcinomas with activating mutations in EGFR (epidermal growth factor receptor) often respond to treatment with EGFR tyrosine kinase inhibitors (TKIs), but the magnitude of tumour regression is variable and transient. This heterogeneity in treatment response could result from genetic modifiers that regulate the degree to which tumour cells are dependent on mutant EGFR. Through a pooled RNA interference screen, we show that knockdown of FAS and several components of the NF-κB pathway specifically enhanced cell death induced by the EGFR TKI erlotinib in EGFR-mutant lung cancer cells. Activation of NF-κB through overexpression of c-FLIP or IKK (also known as CFLAR and IKBKB, respectively), or silencing of IκB (also known as NFKBIA), rescued EGFR-mutant lung cancer cells from EGFR TKI treatment. Genetic or pharmacologic inhibition of NF-κB enhanced erlotinib-induced apoptosis in erlotinib-sensitive and erlotinib-resistant EGFR-mutant lung cancer models. Increased expression of the NF-κB inhibitor IκB predicted for improved response and survival in EGFR-mutant lung cancer patients treated with EGFR TKI. These data identify NF-κB as a potential companion drug target, together with EGFR, in EGFR-mutant lung cancers and provide insight into the mechanisms by which tumour cells escape from oncogene dependence. 相似文献
143.
对贵州省兴义市坡岗自然保护区进行陆生贝类资源调查,共获得标本2 954号,经鉴定得66种(8个未定种),分隶于12科32属。区系分析表明:保护区内陆生贝类以东洋界种类为主,占种类总数的89%。东洋界西南区种类,占种类总数的33%;同时也有少数古北界的种类渗透,占种类总数的7%;广分布种类较少,仅占种类总数的4%。 相似文献
144.
郭畅 《重庆工商大学学报(自然科学版)》2021,38(1):113-119
针对所获取的类别不平衡的深沪A股制造业上市公司财务数据,为了预测制造业上市公司信用违约情况,提出基于欠采样改进的Lasso-Logistic模型;首先通过计算WOE和IV值,剔除风险识别能力和稳定性较差的变量,接着从"数据"层面对现有的Lasso-Logistic模型进行批量欠采样处理,最后结合"算法"层面对Lasso... 相似文献
145.
Shengnan Wang Chenguang Yuan Nana Chang Yang Song Huamin Zhang Yanbin Yin Xianfeng Li 《科学通报(英文版)》2021,(9):889-896
Aqueous zinc-based batteries (ZBBs) have great potential as commercial energy storage devices.However,the poor cycling stability of zinc anode under high areal ... 相似文献
146.
针对铝箔剪切机的碎屑收集问题,该文研究基于计算流体力学(CFD)使用负压管收集方法。在分析流场压力-速度耦合分布特征的基础上,探讨了不同管路结构对双支管吸力的影响规律,并给出管路优化设计建议。仿真结果表明:设置2个挡板时,第2个支管可形成有效负压及吸力,但在第1个支管附近会形成较大的低速回流区,气流流出支管;当2个支管共用1个出口时,2个支管均可产生负压及吸力,但支管与出口距离不一致时会导致支管速度差异达72.17%;优化后的等距型管路结构的支管速度差异降低至约2%,可在支管口形成均匀吸力,满足工业需求;随着挡板高度增加,支管口速度值、支管口速度差异分别呈现增大、减小趋势;支管与主管倒角设置对气流有良好的导向作用,支管速度可提高约9.64%。 相似文献
147.
Jieting Zhang Yan Wang Xiaohua Jiang Hsiao Chang Chan 《Cellular and molecular life sciences : CMLS》2018,75(10):1737-1756
Mutations of cystic fibrosis transmembrane conductance regulator (CFTR) cause cystic fibrosis, the most common life-limiting recessive genetic disease among Caucasians. CFTR mutations have also been linked to increased risk of various cancers but remained controversial for a long time. Recent studies have begun to reveal that CFTR is not merely an ion channel but also an important regulator of cancer development and progression with multiple signaling pathways identified. In this review, we will first present clinical findings showing the correlation of genetic mutations or aberrant expression of CFTR with cancer incidence in multiple cancers. We will then focus on the roles of CFTR in fundamental cellular processes including transformation, survival, proliferation, migration, invasion and epithelial–mesenchymal transition in cancer cells, highlighting the signaling pathways involved. Finally, the association of CFTR expression levels with patient prognosis, and the potential of CFTR as a cancer prognosis indicator in human malignancies will be discussed. 相似文献
148.
Sung LY Gao S Shen H Yu H Song Y Smith SL Chang CC Inoue K Kuo L Lian J Li A Tian XC Tuck DP Weissman SM Yang X Cheng T 《Nature genetics》2006,38(11):1323-1328
Since the creation of Dolly via somatic cell nuclear transfer (SCNT), more than a dozen species of mammals have been cloned using this technology. One hypothesis for the limited success of cloning via SCNT (1%-5%) is that the clones are likely to be derived from adult stem cells. Support for this hypothesis comes from the findings that the reproductive cloning efficiency for embryonic stem cells is five to ten times higher than that for somatic cells as donors and that cloned pups cannot be produced directly from cloned embryos derived from differentiated B and T cells or neuronal cells. The question remains as to whether SCNT-derived animal clones can be derived from truly differentiated somatic cells. We tested this hypothesis with mouse hematopoietic cells at different differentiation stages: hematopoietic stem cells, progenitor cells and granulocytes. We found that cloning efficiency increases over the differentiation hierarchy, and terminally differentiated postmitotic granulocytes yield cloned pups with the greatest cloning efficiency. 相似文献
149.
150.
作为纪录历史的档案文献,是党和国家的宝贵财富。学校档案工作是我国教育事业的重要组成部分,对学校教育教学管理和国家经济建设有着及其重要的作用。文章从梳理学校档案工作的概念和作用入手,对中等专业学校档案工作存在问题进行分析,提出一些加强这项工作的措施,以求科学、规范地做好中等专业学校档案工作。 相似文献