The chick ovomucoid gene contains at least six intervening sequences.

A 15-kilobase pair EcoRI chick DNA fragment, containing both the termination codon UGA and the 5'-portion of the structural ovomucoid gene, has been cloned in lambda phage Charon 4A by in vitro packaging. Restriction mapping and electron microscopic analyses of this cloned DNA have revealed that the structural ovomucoid gene sequences are separated by at least six intervening sequences.     

Detection of marijuana smoke in the atmosphere of a room

Zusammenfassung Mit einer Analysenmethode gelingt es, Marijuanarauch von einer Zigarette oder weniger durch Kombination von Anreicherungsverfahren mit hochauflösender Gaschromatographie in Zimmerluft nachzuweisen. Charakteristische Profile von 200 bis 300 Komponenten (darunter Cannabinoide), die sich deutlich von denen von Tabakrauch unterscheiden, wurden mit Hilfe von Glaskapillarsäulen gewonnen.     

Improved method of large scale purification of acetylcholinesterase from the electric eel (Electrophorus electricus) by affinity chromatography

Résumé Une méthode basée sur d'affinité chromatographique nous a permis de purifier complètement l'acétylcholinestérase des organes électriques du gymnote (Electrophorus electricus). L'activité spécifique de l'acétylcholinestérase ainsi établie en milligrammes dépasse 950 mM de substrat hydrolysé (acétylcholine)/mg protéine/h et sa pureté a été vérifiée par électrophorèse sur gel de polyacrylamide.

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This work was supported by grants: USPHS No. GM-01839 and U.C. San Francisco Academic Senate Research Committee, Grant No. 46.     

Enhancing effect ofBordetella pertussis and propranolol on experimental immune hemolytic anemia

Résumé Un constituant deBordetella pertussis, et la drogue antagoniste, le propranolol-adrénergique, peuvent causer un renforcement semblable similaire de la sensibilité des souris à l'anémie hémolytique immune expérimentale. Il est connu que l'insuline se comporte de la même façon.

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This work was supported in part by Public Health Service Research Grant No. CC00223 from the National Communicable Disease Center through the Massachusetts Health Research Institute.     

Elektronenmikroskopische Beobachtungen bei experimenteller Poliomyelitis

Summary The lumbar motor columns of a cynomolgus monkey which had become tetraplegic after infection with a highly virulent strain of type 3 poliovirus were examined by electron microscopy. Within the cytoplasm of many endothelial cells of intraspinal blood vessels, as well as within the perikarya of numerous mononuclear inflammatory cells, there occurred regular arrays of dense particles which, on the basis of morphological evidence, could be identified as poliovirus crystals. The possible significance of virual replication by endothelial and mononuclear cells for poliovirus spread within the central nervous system is briefly discussed.