An HTLV-III peptide produced by recombinant DNA is immunoreactive with sera from patients with AIDS

Human T-cell lymphotropic retrovirus type III (HTLV-III), also called lymphadenopathy-associated virus (LAV), has been identified as the aetiological agent of acquired immune deficiency syndrome (AIDS). The sera of most patients with AIDS or AIDS-related complexes, and of asymptomatic individuals infected with HTLV-III, contain antibodies against antigens of HTLV-III. The characterization of these antibodies and their corresponding viral antigens is important not only for understanding immunity against HTLV-III and the pathology of AIDS, but also for the development of diagnostic methods and preventive vaccine for AIDS. Following the successful establishment of a long-term T-cell line permissive for HTLV-III replication, large quantities of virus have been produced, facilitating the purification of viral proteins and the development of mouse monoclonal antibodies against several viral antigens. More recently, the structure of HTLV-III proviral DNA has been elucidated. We now report the production, by genetic engineering methods, of a peptide encoded by a gene segment of HTLV-III. A 1.1-kilobase (kb) EcoRI DNA segment from an isolate of HTLV-III was inserted into a lpp and lac promoter-coupled expression vector, pIN-III-ompA. Escherichia coli transformants of this plasmid produced a peptide of relative molecular mass (Mr) 15,000 (15K) which was strongly immunoreactive with anti-HTLV-III antibodies present in sera from AIDS patients. Lysates of the clones expressing this 15K peptide inhibited the reactivity of the p31 virion protein with AIDS sera, suggesting that it is a fragment of the viral p31 protein. The peptide reacted with sera from all 20 AIDS patients but none of the 8 normal controls tested. These results suggest that the peptide may be useful for detecting anti-HTLV-III antibodies in blood samples.     

我国南方九孔鲍工厂化养殖可持续发展的探讨

综述了九孔鲍工厂化养的现状和存在问题，并对九孔鲍养殖业走出困境，恢复持续发展提出四点看法：１）减少污染，保持良好的生态环境；２）提倡养殖废水的综合利用；３）加强的鲍的健康苗的种培育及交育种研究工作；４）加强鲍病的综合防治。     

风积沙筑路材料的击实特性及压实工艺

文章以试验为基础,通过理论上分析,探讨风积沙击实曲线与常规筑路材料不同的原因,并分析风积沙击实特性,进而寻求合理的压实工艺.     

Application-Oriented Performance Comparison of 802.11p and LTE-V in a V2V Communication System

In recent years, the Vehicle-to-Vehicle(V2V) communication system has been considered one of the most promising technologies to build a much safer and more efficient transportation system. Both simulation and field test have been extensively performed to evaluate the performance of the V2V communication system. However,most of the evaluation methods are communication-based, and although in a transportation environment, lack a V2V application-oriented analysis. In this study, we conducted real-world tests and built an application-oriented evaluation model. The experiments were classified into four scenarios: static, following, face 2 face, and crossing vertically, which almost covered all the V2V communication patterns on the road. Under these scenarios, we conducted experiments and built a probability model to evaluate the performance of 802.11p and LTE-V in safetyrelated applications. Consequently, we found out that improvements are still needed in Non-Line-of-Sight scenarios.     

粤港澳大湾区水环境状况分析及治理对策初探

通过梳理粤港澳大湾区河流、水库、近岸海域的水体污染现状, 分析城市内涝、河流及入海口水量变化问题, 发现湾区水体污染主要由大城市密集的人口和工业活动造成, 不完善的污水处理系统和管理体系使水体污染进一步加剧; 对生态用水的过度侵占是水量问题产生的主要原因。通过总结国际先进湾区完善立法、提高标准、控制排放和保护生态的水环境治理经验, 对粤港澳大湾区水环境的进一步治理提出对策: 1) 提升水资源利用效率, 加强污水处理; 2) 加强海绵城市建设; 3) 控制围垦, 加强生态保护; 4) 加快推进社会经济转型。     

再生水脱氮工艺中碳源投加的优化研究

对某再生水厂两级生物滤池脱氮工艺的甲醇投加量进行优化,在2年的实际监测数据基础上,采用线性分析拟合得到甲醇投加量与进/出水总氮质量浓度、水处理量、日均温度等因素的相关方程,为一线工人确定精准投加甲醇量提供了便捷的操作指导。实际运行1年后,出水指标全部合格,甲醇-总氮去除比达4.16。采用以上优化措施降低了脱氮过程中甲醇的投用量,节约了甲醇投用成本。     

汽轮发电机组超速保护对机组热力系统的影响

利用仿真软件对某发电机组的超速保护系统进行建模,模拟机组甩负荷和转子超速后功率负荷不平衡保护和超速保护的动作特性,分析超速保护动作对机组热力系统的影响.结果表明:当机组功率负荷不平衡保护和转子超速保护动作时,快关高调阀和中调阀,会导致阀前主蒸汽压力迅速升高,升至额定压力的1.1415倍,对机组阀门产生一定的冲击力,甚至导致安全阀超压,影响锅炉燃烧.功率负荷不平衡保护存在的唯一优点在于机组超速时能早于超速保护动作,但两种情况对控制超速的效果相差不大,相比较而言,误动作带来的危害更大.      

基于不确定性测度的直觉模糊决策模型

在综合衡量直觉模糊数自身所具有的不确定性及其所表达信息的不确定性的基础上,以凸组合的形式设计了混合型直觉模糊熵和交叉熵测度;与已有测度相比,混合型熵测度具有更强的区分能力,而混合型交叉熵测度具有自然的对称性。在此基础上,设计了直觉模糊环境下的多属性群体决策模型,并将其应用于教育教学质量评价。理论分析和数值结果表明：混合型直觉模糊熵和交叉熵测度能够成对设计,互为印证,因而更具合理性;基于不确定性测度的群体决策模型结构清晰,步骤简明,决策结果客观有效。     

用蒸发法制备NEA光电阴极初探

用蒸发法制备负电子亲和势(NEA)光电阴极,探讨了两个可能性:(一)改进现有多碱阴极的工艺规范,制备负电子亲和势光电阴极。(二)用蒸发法制备Ⅲ—Ⅴ族负电子亲和势光电阴极。     

假设检验的偏好性

根据假设检验的基本原理 ,给出 3种单侧假设检验在相同的显著性水平下具有相同的拒绝域 ,并指出在实际应用中如何选择原假设和备择假设及其相互关系 .