Affinity chromatographic preparation of arterial heavy meromyosin subfragment-1.

Heavy meromyosin subfragment-1 (HMM S-1) was prepared by papain digestion of arterial myosin or actomyosin and was purified by agarose-ATP affinity chromatography. Proteolysis of crude arterial myosin suspensions was preceded by solubilization. HMM-S-1 thus obtained consisted mainly of a 90,000 dalton polypeptide and fully retained the K+- and Ca2+-ATPase of the parent myosin. Its affinity to agarose-ATP was comparable to that of skeletal muscle HMM S-1.     

Occurance of aldehyde-fuchsin and performic acid-victoria blue positive granules in the ovarian pedicle of Dysdercus koenigii F. (Pyrrhocridae: Heteroptera).

A cycle of activity of aldehyde-fuchsin and performic acid-Victoria blue positive granules was observed in the ovarian pedicle of Dysdercus koenigii during the first ovipositional cycle. The quantitative variation of these granules in the pedicle can also be correlated directly with the increase or decrease of the neurosecretory material in the A-type cells of the pars intercerebralis medialis region of the protocerebrum of the brian.     

Influence of the visual cortex upon collicular evoked responses in the rabbit.

In rabbit, the depression of the visual cortex by KCl showed an enhancement of the collicular evoked potentials. This enhancement revealed that the visual cortex had exerted a tonic type influence upon the superior colliculus.     

Biochemical markers in the taxonomy of the Actinomycetales.

Useful biochemical markers for the classification of Actinomycetales are: 1. DL- or LL-diaminopimelic acid in the peptidoglycan, 2. sugar composition of polysaccharides, 3. fatty acid spectrum of cell lipids. The occurrence of various kinds of branched fatty acids and of unsaturated fatty acids is of special value.     

Age and peritoneal fluid cellular distribution in women 20-40 years of age.

Cytologic aspiration specimens of peritoneal fluid revealed that mesothelial cell proportions were significantly reduced 19.2% in women between 26 and 35 years of age. Possibly, mesothelial cell renewal was decreased in women of the older age groups.     

The accumulation pattern of ingested gossypol in selected organs of the rat.

Bound gossypol levels in the spleen and kidney of rats ingesting dietary gossypol (0.98%) varied directly with the feeding intervals of 6, 14, 28 and 35 days. Free gossypol level in the kidney, spleen and lungs increased for 14 days and then tended to decrease as the feeding period was extended.     

The identification of dansyl sarcosine and its occurrence in molluscs.

An unknown dansyl derivative was identified as dansyl sarcosine. In molluscs, sarcosine was found to be largely localized to the nervous system. Examination of individual snail neurones, regions of the octopus brain and the squid giant synapse showed dramatic variations in sarcosine levels.