Variability within and between strains for mating behaviour parameters inDrosophila pseudoobscura

Résumé Chez un caryotype donné deDrosophila pseudoobscura on constate dans quelques lignées plus de variabilité dans la durée du comportement sexuel précédant l'accouplement que dans la durée de celui-ci.

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Supported by the Commonwealth of Australia Scholarship and Fellowship Plan.     

A structural state of the myosin V motor without bound nucleotide

The myosin superfamily of molecular motors use ATP hydrolysis and actin-activated product release to produce directed movement and force. Although this is generally thought to involve movement of a mechanical lever arm attached to a motor core, the structural details of the rearrangement in myosin that drive the lever arm motion on actin attachment are unknown. Motivated by kinetic evidence that the processive unconventional myosin, myosin V, populates a unique state in the absence of nucleotide and actin, we obtained a 2.0 A structure of a myosin V fragment. Here we reveal a conformation of myosin without bound nucleotide. The nucleotide-binding site has adopted new conformations of the nucleotide-binding elements that reduce the affinity for the nucleotide. The major cleft in the molecule has closed, and the lever arm has assumed a position consistent with that in an actomyosin rigor complex. These changes have been accomplished by relative movements of the subdomains of the molecule, and reveal elements of the structural communication between the actin-binding interface and nucleotide-binding site of myosin that underlie the mechanism of chemo-mechanical transduction.     

Atom-molecule coherence in a Bose-Einstein condensate

Recent advances in the precise control of ultracold atomic systems have led to the realisation of Bose Einstein condensates (BECs) and degenerate Fermi gases. An important challenge is to extend this level of control to more complicated molecular systems. One route for producing ultracold molecules is to form them from the atoms in a BEC. For example, a two-photon stimulated Raman transition in a (87)Rb BEC has been used to produce (87)Rb(2) molecules in a single rotational-vibrational state, and ultracold molecules have also been formed through photoassociation of a sodium BEC. Although the coherence properties of such systems have not hitherto been probed, the prospect of creating a superposition of atomic and molecular condensates has initiated much theoretical work. Here we make use of a time-varying magnetic field near a Feshbach resonance to produce coherent coupling between atoms and molecules in a (85)Rb BEC. A mixture of atomic and molecular states is created and probed by sudden changes in the magnetic field, which lead to oscillations in the number of atoms that remain in the condensate. The oscillation frequency, measured over a large range of magnetic fields, is in excellent agreement with the theoretical molecular binding energy, indicating that we have created a quantum superposition of atoms and diatomic molecules two chemically different species.     

Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase

Protein kinases are involved in stress signalling in both plant and animal systems. The hormone abscisic acid mediates the responses of plants to stresses such as drought, salinity and cold. Abscisic-acid-activated protein kinase (AAPK -- found in guard cells, which control stomatal pores -- has been shown to regulate plasma membrane ion channels. Here we show that AAPK-interacting protein 1 (AKIP1), with sequence homology to heterogeneous nuclear RNA-binding protein A/B, is a substrate of AAPK. AAPK-dependent phosphorylation is required for the interaction of AKIP1 with messenger RNA that encodes dehydrin, a protein implicated in cell protection under stress conditions. AAPK and AKIP1 are present in the guard-cell nucleus, and in vivo treatment of such cells with abscisic acid enhances the partitioning of AKIP1 into subnuclear foci which are reminiscent of nuclear speckles. These results show that phosphorylation-regulated RNA target discrimination by heterogeneous nuclear RNA-binding proteins may be a general phenomenon in eukaryotes, and implicate a plant hormone in the regulation of protein dynamics during rapid subnuclear reorganization.     

Points of control in inflammation

Inflammation is a complex set of interactions among soluble factors and cells that can arise in any tissue in response to traumatic, infectious, post-ischaemic, toxic or autoimmune injury. The process normally leads to recovery from infection and to healing, However, if targeted destruction and assisted repair are not properly phased, inflammation can lead to persistent tissue damage by leukocytes, lymphocytes or collagen. Inflammation may be considered in terms of its checkpoints, where binary or higher-order signals drive each commitment to escalate, go signals trigger stop signals, and molecules responsible for mediating the inflammatory response also suppress it, depending on timing and context. The non-inflammatory state does not arise passively from an absence of inflammatory stimuli; rather, maintenance of health requires the positive actions of specific gene products to suppress reactions to potentially inflammatory stimuli that do not warrant a full response.     

Einstein's struggle for a Machian gravitation theory

The story of Einstein's struggle to create a general theory of relativity, and his early discontentment with the final form of the theory (1915), is well known in broad outline. Thanks to the work of John Norton and others, much of the fine detail of the story is also now known. One aspect of Einstein's work in this period has, however, been relatively neglected: Einstein's commitment to Mach's ideas on inertia, and the influence this commitment had on Einstein's work on general relativity from 1907 to 1918. In this paper published writings and archival material are examined, to try to reconstruct the details of Einstein's thinking about inertia and gravitation, and the role that Mach's ideas played in Einstein's crucial work on the general theory. By the end, a clear picture of Einstein's conceptions of Mach's ideas on inertia, and their philosophical motivations, will emerge. Several surprising conclusions also emerge: Einstein's desire for a Machian gravitation theory was the central force driving his work from 1912 to 1915, keeping him going despite numerous frustrating setbacks; Einstein's continued commitment to Mach's ideas in 1916–1917 kept him at work trying various strategies of modification of the field equations, in order to exclude anti-Machian solutions (including the addition of the cosmological constant in 1917); and as late as early 1918, Einstein was ready to call the whole General Theory a failure if no way of squaring it with Mach's ideas on inertia could be found. But by 1920 Einstein advocated a view that granted spacetime (under the name ‘ether’) independent existence with physical qualities of its own, a complete break with his earlier Machian views.