Orchid bees (Apidae: Euglossini) in Cerrado remnants in northeast Brazil

There is a general lack of information concerning the diversity of bees belonging to the Euglossini tribe in Cerrado areas closest to the Brazilian Amazon. The state of Maranhão is situated in the northeast Brazilian Cerrado and has become the agricultural frontier of the country due to the advancement of monoculture and cattle farming. These activities have suppressed animal and plant populations in large areas of the Cerrado remnants, for which we have not yet obtained adequate scientific knowledge of local species. The objective of this study was to conduct a survey of Euglossini fauna in the northeast Cerrado. We evaluated the variation in species richness, composition and abundance between two distinct vegetation types: Cerrado sensu stricto (s.s.) and gallery forest. Male bees were captured from 7:00 a.m. to 5:00 p.m. over two consecutive days. Captures were carried out once per month from July 2012 to December 2013, using a combination of passive and active collection techniques, including baited traps, as well as a collector with an entomological net to collect bees near traps. We collected a total of 766 Euglossini males belonging to 24 species and five genera. The most abundant species were Eulaema bombiformis, Eulaema nigrita and Eulaema cingulata for the gallery forest site, whereas Eulaema nigrita, Euglossa melanotricha and Euglossa cordata were more abundant in the Cerrado s.s. The gallery forest yielded a higher number of male Euglossini (n = 503, 21 species) compared with the Cerrado s.s. (n = 263, 16 species). The presence of seven exclusive species in the gallery forest and three in the Cerrado s.s. indicated that both environments are important for the maintenance of Euglossini species in this region and highlight the increasing need for conservation programmes for the protection of Cerrado environments.     

Reproductive biology of two species of Hyalella Smith, 1874 (Crustacea: Amphipoda: Hyalellidae) from southern Brazil

Reproduction is a critical event in the life cycle of animals, and a high amount of energy is allocated to it. The aim of this study was to characterize the reproductive biology of two recently described species of Hyalella from the north-east region of state of Rio Grande do Sul in southern Brazil. The specimens were collected during 12 months from a stream source (Hyalella georginae) and from a water reservoir (H. gauchensis), in Palmeira das Missões municipality, state of Rio Grande do Sul. Upon sampling, ovigerous females and pairs in precopulatory behaviour were separated into microtubes. In the laboratory, the cephalothorax length (CL in mm) of all specimens was measured. Male size at onset of sexual maturity was larger than that of the females, in both species. There was a correlation between the size of paired males and females: larger males carried larger females, in both Hyalella species. Precopulatory pairs were found throughout the year; however, H. georginae had a higher frequency in spring, and H. gauchensis in autumn. There was a positive correlation between the CL of ovigerous females and the number of eggs/juveniles. A reduction in the number of eggs throughout the embryonic development was observed in H. georginae. The average fecundity of H. georginae was significantly higher than H. gauchensis. The differences between the reproductive biology of these species may be related to the physical and environmental conditions at which they are exposed in their habitats.     

Heterospecific mating between distantly related species of stink bugs and its evolutionary implications

The copulatory process in animals is preceded by a series of integrated communication processes which are often under selection to guarantee species-specific courtship and copula. However, individuals of species supposedly in reproductive isolation frequently copulate in nature, which has sparked the attention of evolutionary biologists for decades. While studies on this topic have strongly focused on recently diverged species or populations, few instances of heterospecific copula between distantly related species are known. Here we report an intriguing case of interspecific copula between two distantly related species of stink bugs in nature. We replicated this observation in laboratory conditions and revealed an asymmetric relation: males of Chinavia obstinata copulated with females of C. erythrocnemis but the reverse combination did not occur. We propose a series of hypotheses to explain our observations in the light of the current knowledge on mate recognition and reproductive isolation in stink bugs, considering the potential pre- and postcopulatory barriers involved. Once these two species have probably evolved in allopatry and contacted secondarily, the absence of directional selection against mating signals differentiation is a probable process. We argue that similarities in genital shape and size, most likely a result of convergent evolution, may have played a fundamental role in guaranteeing that these species achieved proper coupling.     

Population dynamics of the bark-dwelling spider Eustala perfida Mello-Leitão, 1947 (Araneidae)

Population dynamics of spiders may vary according to biotic and abiotic factors. We studied the phenology of the bark-dwelling spider Eustala perfida over a two-year period and investigated how temperature, precipitation, and prey availability were associated with the population of this species. The population of E. perfida presented a well-defined fluctuation, with one reproductive cycle per year. The recruitment of spiders occurred in summer, followed by successive phenological peaks at all stages of development. The species is protogynic, and the population of adult males peaks in the cold/dry season, indicating a winter mature phenological pattern. The diet of E. perfida is composed mainly of medium-sized homopterans, complemented by small dipterans and hymenopterans. We found that the higher abundance of spiders occurred two months after the highest values of precipitation and temperature, and three months after the higher abundance of potential prey items. These results indicate that spiders require time to respond to changes in environmental conditions. The species’ natural history and the structure of the trunks in which it lives may also affect the number of individuals in a particular area and over time.     

Identification of Megaselia scalaris (Loew, 1866) (Diptera: Phoridae) in mummified human body from Itacambira (MG), Brazil,using scanning electron microscopy and cuticular hydrocarbons

Insect puparia were found adhered to the ribs and other tissues in the abdominal cavity of a natural male mummy found in Itacambira (State of Minas Gerais, Brazil) dating to the Colonial Period. They were collected for identification by scanning electron microscopy, and for comparison of several morphological features with those described in the literature. The puparia were found open and dorsoventrally flattened, making it difficult to visualize the dorsal projections. The tegument is covered by tapered spines and contains rows of small tubercles on the dorsal and lateral regions of the puparium. The posterior spiracle consists of four parallel openings arranged in pairs. These results are indicative that the specimens belong to the species Megaselia scalaris (Loew, 1866) (Diptera: Phoridae). Additionally, cuticular hydrocarbons of the puparia were analysed by gas chromatography coupled to mass spectrometry and compared with the profile of M. scalaris reared in the laboratory.     

Filamentous bodies in human glomerulonephritis

Filamentous bodies have been identified in the glomerular cells of human kidney biopsies. These structures have a close morphological resemblance to ciliary rootlets, although the cells of the glomerular tuft only occasionally bear cilia. Their significance could be, as for cilia, of a cellular disdifferentiation of a pathological cellular proliferation.     

Management of Waste Electrical and Electronic Equipment in Brazilian Public Education Institutions: Implementation Through Action Research on a University Campus

One of the problems facing public education institutions in Brazil is the question of the correct handling and treatment of waste electrical and electronic equipment (WEEE). Being that these institutions are part of the public sector, they should adhere to Decree no. 99,658/1990 for the disposal of their goods. However, this decree is not completely in accordance with the Brazilian National Solid Waste Policy (PNRS), especially when considering the regulations for disposal of public goods. Thus, these institutions have no WEEE management model which encompasses not only the Decree but also the PNRS. An example of this situation can be found at the Federal University of Itajubá (UNIFEI) in Minas Gerais, Brazil. Due to the lack of such a management model, the university warehouse is at full capacity and unable to receive additional electronic equipment. Therefore, the objective of this study is to create a management model for WEEE to be used at public education institutions using the guidelines set forth in both the Decree and the PNRS. An action research investigation was performed at UNIFEI which relied upon two improvement and learning cycles to deal with all the WEEE found at the university. The first was undertaken at the university warehouse and the second at other sectors within the institution. In addition to the practical results obtained by properly disposing of 474 WEEE products, this study showed that action research is an adequate management tool for public institutions looking to deal with problems of this nature, being that these institutions are almost always subject to bureaucratic controls with respect to their public property, and expected to follow rigorous legislation that is often times controversial.