Cupric ion release and cytotoxicity for Yuangong Cu-IUDs and the release behavior of indomethacin for medicated 220 Cu-IUD

These years Yuangong copper-bearing intrauterine devices （Cu-IUDs） have been used because of less side effects in use. The corrosion of copper is essential to the success of contraception, and the release behavior of indomethacin from medicated Cu-IUD is related to its therapeutic effect. In this study, analytical methods were established to investigate the release behavior of cupric ion of three kinds of Yuangong Cu-IUDs and indomethacin of medicated Yuangong 220 Cu-IUD. Cu-IUDs were incubated in simulated uterine solution （SUS）. The concentrations of cupric ion and indomethacin were analyzed by flame atomic absorption spectrometer （FAAS） for 60 days and UV/vis -3310 spectrophotometer for 60 days, respectively. The morphology of copper after corrosion was characterized by SEM. In addition, we detected cytotoxicity by MTT of L929 mouse fibroblasts cells caused by extracts of the three Yuangong Cu-IUDs. The release behavior of cupric ion for three kinds of Yuangong Cu-IUDs was biphasic, which consisted of the initial burst release and then slow and constant release. In vitro release experiment confirmed a biphasic release of indomethacin from Yuangong 220. The copper wire of Yuangong Cu-IUDs showed uneven corrosion. The RGR value of Yuangong 365 Cu-IUD was smaller than that of medicated Yuangong 220 Cu-IUD and RGR value of medicated Yuangong 220 Cu-IUD was smaller than that of Yuangong 300 Cu-IUD. The cupric ion release and indomethacin release showed biphasic. Indomethacin increased the cupric ion release rate and might diminish the adverse effects caused by burst release of cupric ion. The toxicity grade of these three Yuangong Cu-IUDs was 4. We should canvass the adverse events of Cu-lUDs based on practical experiments, and try our best to reduce the toxicity of Cu-lUDs.     

一种基于区分服务的IEEE 802.11 QoS信道访问控制机制

研究表明,无线移动自组织网络具有带宽有限,信道质量不稳定,节点移动造成网络拓扑结构变化等诸多特性,这些特性严重损害了网络性能,以致在传统无线移动网络中支持具有严格QoS要求的多媒体应用面临严峻的挑战.本文提出了一种基于区分服务的信道接入控制机制,以更好地支持实时通信流.该机制对传统的IEEE 802.11DCF机制进行...     

扇叶铁线蕨总黄酮和总酚含量及其抗氧化能力分析

测定了扇叶铁线蕨(Adiantum flabellulatum L.)的总黄酮和总酚含量及其对DPPH(DPPH˙)自由基、ABTS自由基(ABTS˙)的清除力和Fe的还原力.结果表明:1)扇叶铁线蕨总黄酮质量分数在6%~8%之间,总酚质量分数在7%~14%之间,地下部分总黄酮和总酚含量高于地上部分,不同产地的扇叶铁线蕨总黄酮和总酚含量存在一定差异;2)扇叶铁线蕨提取物具有一定的Fe还原能力,以及清除DPPH自由基和ABTS自由基的能力;且ABTS自由基清除能力和Fe还原能力随总黄酮和总酚浓度的增加而增强.     

Preparation of the selenomethionine derivative of tabtoxin resistance protein

In order to obtain phase information for the X-ray diffraction of tabtoxin resistance protein (TTR) crystal using the MAD phasing method, a selenomethionine (Se-Met) derivative of TTR was overexpressed in E. coli strain M15, with pQE-30 plasmid, through IPTG induction in M9 medium containing Se-Met. The product was purified to an estimated homogeneity of greater than 95% according to SDS-PAGE, by a Ni-NTA metal affinity followed by a Mono Q anion exchange column chromatography. The successful substitution of Se-Met for methionine (Met) was confirmed by MALDI-TOF and ESI-Quadrupole Mass Spectrometry analysis. The derivative crystal was obtained using similar conditions as those for the native.     

《现代汉语词典》第5版家具类词计量研究

现代汉语家具类词颇具特色。在语音形式上,家具类词语以单音节和双音节居多,三音节相对较少,四音节很罕见。在词义架构上,单义词占优势,多义词只占少数一部分,词义架构简单。从词的内部语素构成来看,家具类词语中合成词占有绝对的优势。单纯词中以单音节单纯词为主。合成词中不存在重叠式合成词。复合式合成词中,偏正型占有主要地位,联合型次之。附加式合成词中,以"词根+词缀"型为主。《现汉》在家具类词语的诠释中较之《汉语大词典》和《现代汉语规范词典》还存在一些有待改善的地方,尤其在遵循统一性方面还有许多问题可以讨论。     

利用RBF神经网络实现聚合反应的内模控制

文中研究基于径向基(RBF)神经网络算法的内模控制策略在苯乙烯本体聚合反应相对分子质量分布控制领域的应用。利用神经网络对非线性系统的逼近能力，把内模控制推广到聚合反应过程质量指标控制这一非线性系统中。针对建模过程中存在的稳态误差，在训练数据中增加了部分静态数据，有效的提高了模型的验证精度，大大改善了由神经网络构成的内模控制器的控制精度，消除了系统余差。仿真结果证明，基于神经网络算法的内模控制策略达到了较好的控制质量。     

一种聚丙烯酰胺凝胶的快速染色方法——热考染法

在蛋白的染色方法中,目前以考马斯亮蓝染色最为常用.以樟子松的针叶为材料,以加热的考马斯亮蓝R-350染液和未经加热的考马斯亮蓝R-350染液以及未经加热的考马斯亮蓝R-250染液进行染色法方法的比较,从而证明热考染法具有蛋白点清晰、背景及对比度良好、安全、操作便捷等较优的染色特点.     

加强机关事业单位工会组织规范化

就机关事业单位工会组织的规范化,论述了要以强化民主管理工作为重点的规范化建设,全面提高其工作水平,促进机关事业单位的决策科学化、民主化和民主政治建设,调动干部职工的积极性、创造性,增强机关事业单位凝聚力,推动各项工作圆满完成.     

Improved Approximate Minimum Degree Ordering Method and Its Application for Electrical Power Network Analysis and Computation

Electrical power network analysis and computation play an important role in the planning and operation of the power grid, and they are modeled mathematically as differential equations and network algebraic equations.The direct method based on Gaussian elimination theory can obtain analytical results. Two factors affect computing efficiency: the number of nonzero element fillings and the length of elimination tree. This article constructs mapping correspondence between eliminated tree nodes and quotient graph nodes through graph and quotient graph theories.The Approximate Minimum Degree(AMD) of quotient graph nodes and the length of the elimination tree nodes are composed to build an Approximate Minimum Degree and Minimum Length(AMDML) model. The quotient graph node with the minimum degree, which is also the minimum length of elimination tree node, is selected as the next ordering vector. Compared with AMD ordering method and other common methods, the proposed method further reduces the length of elimination tree without increasing the number of nonzero fillings; the length was decreased by about 10% compared with the AMD method. A testbed for experiment was built. The efficiency of the proposed method was evaluated based on different sizes of coefficient matrices of power flow cases.     

半线性二阶微分方程周期边值问题

本文利用整体反函数理论,研究了半线性二阶微分方程周期边值问题,给出了周期解存在唯一性的充分条件.