Archaeal acylamino acid releasing enzyme/lipase:Crystallization and preliminary crystallographic analysis in a new crystal form

A primitive orthorhombic crystal form of acylamino acid releasing enzyme‘lipase(APE1547)from hyperthermophilic archaeon Aeropyrum pernix strain K1 has been obtained at 291K.The diffraction pattern of the crystal extends to 0.27nm resolution at 100K using Cu Kαradiation.The crystal belongs to the space group P212121 with unit cell dimensions of b=6.399,b=10.439and c=16.953nm.The presence of two molecules per asymmetric unit gives a crystal volume per protein mass(Vm)of 0.0022nm^3 Da^-1 and a solvent content of 43% by volume.A full set of X-ray diffraction data were collected to 0.3nm from the native crystal.     

Initial organic geochemical investigation on Late Neoproterozoic-Early Cambrian sediments in the Yangtze region, China

Totally 19 samples of typical Upper Proterozoic-Lower Cambrian sedimentary rocks were collected and analyzed for an organic geochemical investigation. Almost all these rocks have high TOCs, super-maturities and similar biomarker distribution. As an exception, however, the Sinian Nantuo Tillite shows much lower TOCs and little phytane and pristane in comparison with those in other strata, which implies a very faint photosynthetic process, and a restricted euphotic zone and quite limited sunlight within the sedimentary water column during the Sinian glaciation age in the western Yangtze region providing an evidence for palaeo-oceanic environment of the Neoproterozoic Snowball Earth age.     

论FDI对西部制度变迁的影响及对策

本文首先对我国西部地区利用FDI的现状进行了描述 ,然后运用回归分析的方法分析了FDI和制度变迁的关系 ,指出FDI是影响区域制度变迁的重要因素 ,FDI在区域分布上的不均衡严重制约着西部地区制度变迁的进程。西部地区应该加快体制改革的步伐 ,改善投资环境 ,吸引FDI ,进而通过FDI带动西部地区制度变迁的进程 ,最终形成FDI和制度变迁相互推动、相互促进的良性循环。     

Evolution of the South China Sea and monsoon history revealed in deep-sea records

As the third summary report of ODP Leg 184 to the South China Sea (SCS), this paper discusses the evolution of the East Asian monsoon and the SCS basin. A multi-proxy approach, involving geochemistry, micropale-ontology, pollen and other analyses, was adopted for reconstructing the evolutionary history of the East Asian monsoon, which was characterized by a series of paleo-climate events especially at 8, 3.2, 2.2 and 0.4 Ma. The new record indicates similar stages in the development of the East and South Asian monsoons, with an enhanced winter monsoon over East Asia being the major difference. The rich spectrums of monsoon variability from the southern SCS also reveal other characteristic features of the low latitude ocean. Evidence for the evolution of the SCS includes the hemipelagic Oligocene sediments, implying the existence of deep water environments during the early seafloor spreading stage of the SCS basin. The four major unconformities and some remarkabl ediagenetic features in upper Oligocene deposits indicate the strongest tectonic events in the region. From a careful comparison of lithologies and sedimentation rates, we conclude that the prominent differences in sedimentary environments between the southern and northern SCS were established only by ～3 Ma.     

多媒质区域介质子网格时域有限差分法

介绍了应用于多媒质区域电磁问题的介质子网格时域有限差分法．该方法在保证精度的前提下，节省了大量计算时间及计算机内存，尤其在处理多媒质界面方面具有很大的优势．     

老年人住宅建筑设计的新思考

针对老年人生活行为特征和老年人对住宅建设及住宅功能的需求，阐述了老年住宅建筑设计的原则，提出了老年住宅建筑设计的要点，强调了住宅建筑设计要以人为本和以老年人为核心的设计思想。     

城市污水厂剩余活性污泥农用价值分析

分析了目前国内外污水污泥处置、利用、管理的现状和发展趋势，通过对城市污水处理厂剩余活性污泥成分、利用风险以及污泥农用市场的分析，综合评价了经过无害化、稳定化处置后剩余污泥的农用价值。     

电铲铲斗齿的修复工艺

通过分析铲斗齿的磨损原因，提出了采用堆焊的方法对铲斗齿进行修复。详细介绍了铲斗齿的修复工艺，堆焊材料的选择及堆焊时应注意的事项。实践表明，此修复工艺切实可行，取得了令人满意的效果。     

基于普通编码器的高精度位置检测方法

根据普通增量式光电编码器测量转角位置的原理，分析了量化误差的形成原因和编码器脉宽制造误差对测量精度的影响，提出了新的信号处理算法——脉冲细分法，利用该方法减小了量化误差．同时标定出编码器的脉宽系数井以它作为脉宽制造误差的补偿参数，消除对位置测量造成的影响，最终提高了系统的测量精度．     

P物质对GABAA受体介导电流的作用

应用全细胞膜片箝技术,在大鼠新鲜分离的背根神经节细胞上观察到:SP对GABA激活电流有调制作用。实验结果表明:多数受检的大鼠背根神经节神经元对SP(28/41,68.5％)和GABA(36/41,88.2％)敏感,100μmol/LSP和100μmol/L GABA激活内向电流的幅值分别为243.8±82.7pA(x±s,n=9)和1.76±0.48nA(x±s,n=13)。GABA与SP激活的内向电流明显不同,前者具有明显的去敏感现象。实验预加SP(0.001～1μmol/L)30s后再加GABA,则GABA激活电流的幅值明显减小,而且GABA激活电流减小的程度与预加SP的浓度呈量效依赖关系。SP主要抑制GABA激活电流的峰电流,对其稳态电流的抑制不明显。预加0.1μmol/L SP之后约4min左右,SP对GA-BA激活电流的抑制效率最大,为49.8±7.2％(x±s,n=7,P≤0.01),而预加SP 12min之后,SP才失去对GABA激活电流的抑制作用。