Crystal structure of the neurotrophin-3 and p75NTR symmetrical complex

Neurotrophins (NTs) are important regulators for the survival, differentiation and maintenance of different peripheral and central neurons. NTs bind to two distinct classes of glycosylated receptor: the p75 neurotrophin receptor (p75(NTR)) and tyrosine kinase receptors (Trks). Whereas p75(NTR) binds to all NTs, the Trk subtypes are specific for each NT. The question of whether NTs stimulate p75(NTR) by inducing receptor homodimerization is still under debate. Here we report the 2.6-A resolution crystal structure of neurotrophin-3 (NT-3) complexed to the ectodomain of glycosylated p75(NTR). In contrast to the previously reported asymmetric complex structure, which contains a dimer of nerve growth factor (NGF) bound to a single ectodomain of deglycosylated p75(NTR) (ref. 3), we show that NT-3 forms a central homodimer around which two glycosylated p75(NTR) molecules bind symmetrically. Symmetrical binding occurs along the NT-3 interfaces, resulting in a 2:2 ligand-receptor cluster. A comparison of the symmetrical and asymmetric structures reveals significant differences in ligand-receptor interactions and p75(NTR) conformations. Biochemical experiments indicate that both NT-3 and NGF bind to p75(NTR) with 2:2 stoichiometry in solution, whereas the 2:1 complexes are the result of artificial deglycosylation. We therefore propose that the symmetrical 2:2 complex reflects a native state of p75(NTR) activation at the cell surface. These results provide a model for NTs-p75(NTR) recognition and signal generation, as well as insights into coordination between p75(NTR) and Trks.     

Performance Improvement of Distributed Systems by Autotuning of the Configuration Parameters

The performance of distributed computing systems is partially dependent on configuration parameters recorded in configuration files. Evolutionary strategies, with their ability to have a global view of the structural information, have been shown to effectively improve performance. However, most of these methods consume too much measurement time. This paper introduces an ordinal optimization based strategy combined with a back propagation neural network for autotuning of the configuration parameters. The strategy was first proposed in the automation community for complex manufacturing system optimization and is customized here for improving distributed system performance. The method is compared with the covariance matrix algorithm. Tests using a real distributed system with three-tier servers show that the strategy reduces the testing time by 40% on average at a reasonable performance cost.     

N杂环卡宾在催化有机合成中的应用

1991年,Arduengo等分离出第一个稳定的N杂环卡宾——咪唑-2-碳烯,之后,N杂环卡宾化学得到了迅速的发展,在多种催化反应中得到了广泛的应用.本文综述了N杂环卡宾在催化有机反应中的研究进展,如催化安息香缩合反应、Stetter反应、a3-d3极性反转反应、开环反应、酯基交换反应、开环聚合反应.     

GF(3)上一类几乎平衡的6阶分圆序列

具有良好自相关性的伪随机序列在信息安全等领域中有着广泛的应用.在GF(3)上构造一类周期为p的几乎平衡6阶分圆序列,利用6阶分圆数计算出该序列的自相关值,并进一步给出满足适当条件的素数p,使得这类序列的自相关值为3值.     

使用多方态的量子密钥共享方案(英文)

给出使用Sen等推广的多方对称态的量子密钥共享方案,我们采用不同的方法证明这个方案是安全的.然后,根据局域实在性的破缺,证明了对称的多方量子态的量子密钥共享的安全性,并作了进一步的推广.     

基于AMESim-simulink仿真对混合动力汽车感应电机控制系统性能的研究

本文将直接转矩控制技术应用于混合动力汽车电机控制系统中,用于研究感应电机在混合动力客车中的运行性能,使电机控制系统有较高的响应速度和较强的鲁棒性。同时有效地分析了电机在混合动力汽车中的运行状态,并用matlab/simulink软件建立了直接转矩控制仿真模型,通过AMESim混合动力客车整车模型联合仿真,显示了控制策略在混合动力汽车电机控制系统运行的快速性和稳定性。     

基于AMESim-simulink仿真对混合动力汽车感应电机控制系统性能的研究

本文将直接转矩控制技术应用于混合动力汽车电机控制系统中,用于研究感应电机在混合动力客车中的运行性能,使电机控制系统有较高的响应速度和较强的鲁棒性.同时有效地分析了电机在混合动力汽车中的运行状态,并用matlab/simulink软件建立了直接转矩控制仿真模型,通过AMESim混合动力客车整车模型联合仿真,显示了控制策略在混合动力汽车电机控制系统运行的快速性和稳定性.     

浅议高校图书馆员职业道德

介绍了高校图书馆员职业道德的具体内容,阐述了良好职业道德所体现出的作用,提出了图书馆员提高自身职业道德的途径。     

高校女性图书馆员群体现状调查分析

女性图书馆员作为高校教师构成中占有较大比例的一个特殊群体,研究其群体特征具有重要的意义。从2005年开始,调查跟踪四川省10所高校图书馆女性馆员的现状,采用多种研究方法对她们的工作压力、幸福指数、事业发展状况以及她们对高校人文环境的评价、对社会层面的关注方向等方面进行了综合调查分析,拟对现代高校人才工程的建设、图书馆员知识结构和专业结构的建设有所贡献。     

十二种花类药材中八种微量元素的主成分和因子分析

应用化学计量学方法研究了十二种花类药材中所含微量元素之间的关系,结果表明应用四个主成分可以合理解释微量元素之间的相关关系; 主成分分析和因子得分可以用于十二种不同花类药材质量的综合评价,为花类药材的合理利用提供了可靠依据.