枯草杆菌(B.subtilis)原生质体融合的方法学研究

利用改良的HM制备液,在适宜的条件下酶解制备亲本株的原生质体,在DNase存在的条件下,用PEG(MW6000)做为聚合剂,经低温短时间处理,将枯草杆菌(B.subtilis)Ki—2—132衍生株与BR_(151)衍生株的原生质体融合。再在适宜的条件下使用改良的DM_3再生培养基,补加适量经处理的人血清白蛋白,同时加入适量适合于枯草杆菌细胞壁再生的引物进行再生,使再生率达到了55～58.15％左右。融合率高达1.82×10~(-2)。筛选出51种不同类型的融合子923株,经传代8代测得其融合子的稳定率达32.46％,从而在工业菌株的选育中使获得高产菌株成为可能。     

Application of triple potential step amperometry method for quantitative electroanalysis

A novel quantitative electroanalysis method, triple potential step amperometry (TPSA), was developed and explained with an example of nitrobenzene analyzing in water. The selectivity of TPSA was im- proved by controlling the potential step within a narrow interval and using enzyme-modified electrode, the narrow potential step makes the method avoid most interferents, and enzyme-modified electrode can enhance the response of target substance selectively. The peak area was investigated for quanti- tative calibration, such as nitrobenzene concentration showing a linear relation with the peak area, with the correlation coefficients being 0.9995. The t-test and F-test were applied to evaluating the reliability of TPSA, the results showed that there was no evidence of systematic error for TPSA, and the method was of no significant difference from CV. The merit of fast detecting and few potential changing times make the TPSA suitably applicable to low-cost automatic monitoring equipments.     

Online uniform machine covering with the known largest size

This paper investigates the semi-online scheduling problem with the known largest size on two uniform machines. The objective is to maximize the minimum machine completion time. Both lower bounds and algorithms are given. Algorithms are optimal for the majority values of s≥1, where s is the speed ratio of the two machines. The largest gap between the competitive ratio and the lower bound is about 0.064. Moreover, the overall competitive ratio 2 matches the overall lower bound.     

Decentralized and overall condition monitoring system for large-scale mobile and complex equipment

It is an urgent project to realize online and overall condition monitoring and timely fault diagnosis for large-scale mobile and complex equipment. Moreover, most of the existing large-scale complex equipment has quite insufficient accessibility of examination, although it still has quite a long service life. The decentralized and overall condition monitoring, as a new concept, is proposed from the point of view of the whole system. A set of complex equipment is divided into several parts in terms of concrete equipment. Every part is processed via one detecting unit, and the main detecting unit is connected with other units. The management work and communications with the remote monitoring center have been taken on by it. Consequently, the difficulty of realizing a condition monitoring system and the complexity of processing information is reduced greatly. Furthermore, excellent maintainability of the condition monitoring system is obtained because of the modularization design. Through an application example, the design and realization of the decentralized and overall condition monitoring system is introduced specifically. Some advanced technologies, such as, micro control unit (MCU), advanced RISC machines (ARM), and control area network (CAN), have been adopted in the system. The system's applicability for the existing large-scale mobile and complex equipment is tested.     

价格测算模型解的存在性定理

价格测算模型解的存在性定理曹国钧，王健（重庆医药设计院６３００００）（渝州大学数学系）ＡＴｈｅｏｒｅｍｆｏｒＥｘｉｓｔｅｎｃｅｏｆｔｈｅＳｏｌｕｔｉｏｎｏｎＰｒｉｃｅＥｓｔｉｍａｔｅＭｏｄｅｌＣａｏＧｕｏｊｕｎＷａｎｇＪｉａｎ（ＣｈｏｎｇＱｉｎｇＰｈ...     

SO_4~（2—）／Fe_2O_3型固体超强酸在乙酸异戊酯合成中的应用

Ｆｅ＿２Ｏ＿３型固体超强酸在酯化反应中显示出与浓硫酸相近的催化活性，但具有易与产物分离、腐蚀作用小、污染程度轻和可重复使用的优点。本文考察了Ｆｅ＿２Ｏ＿３型固体超强酸在乙酸异戊酯合成反应中的摧化活性，结果表明，最佳催化剂用量为Ｆｅ＿２Ｏ＿３型固体超强酸占总投料量的２％，最优反应时间为２ｈ，此时异戊醇的转化率可达９４％。     

轮式装载机结构件的强度分析与可靠性分析

本文分析了用SAP5P进行轮式装载机结构件强度分析与可靠性分析的条件,讨论了计算中可能出现的问题,对计算模型的建立,各类部件单元的选取,计算结果的数据处理、应力分布和强度分布函数的确定和选择等提出了一套可行的方法,有很高的实用价值。     

科研成果管理的程序设计

用ｄＢＡＳＥⅢ软件设计了可普遍运行的科研成果管理程序。     

“准平面”法——平面光弹点测法的新方法

本文分析了三维效应对平面光弹技术测量精度的影响,并以阿朋(Aben)的偏振光在光弹性体中传播的三维理论为基础,提出了平面光弹的“准平面”特征以及平面光弹的“准平面”点测法。此方法消除了三维效应所导致的测量误差,提高了测量精度。实验证明新方法是可行的。     

单晶硅表面键合菁染料的表面增强拉曼散射

用表面增强拉曼散射技术研究单晶硅表面键合光敏染料，可获得信噪比强的ＳＥＲＳ图。因染料分子中含有孤对电子的氮原子和起增强作用的银离子相互作用，使Ａｇ＋成为连结染料分子间的锁，增加染料分子极化率，使拉曼散射截面积增大，从而起到增强效果的作用。