Ionic colloidal crystals of oppositely charged particles

Colloidal suspensions are widely used to study processes such as melting, freezing and glass transitions. This is because they display the same phase behaviour as atoms or molecules, with the nano- to micrometre size of the colloidal particles making it possible to observe them directly in real space. Another attractive feature is that different types of colloidal interactions, such as long-range repulsive, short-range attractive, hard-sphere-like and dipolar, can be realized and give rise to equilibrium phases. However, spherically symmetric, long-range attractions (that is, ionic interactions) have so far always resulted in irreversible colloidal aggregation. Here we show that the electrostatic interaction between oppositely charged particles can be tuned such that large ionic colloidal crystals form readily, with our theory and simulations confirming the stability of these structures. We find that in contrast to atomic systems, the stoichiometry of our colloidal crystals is not dictated by charge neutrality; this allows us to obtain a remarkable diversity of new binary structures. An external electric field melts the crystals, confirming that the constituent particles are indeed oppositely charged. Colloidal model systems can thus be used to study the phase behaviour of ionic species. We also expect that our approach to controlling opposite-charge interactions will facilitate the production of binary crystals of micrometre-sized particles, which could find use as advanced materials for photonic applications.     

Role of the HTLV-III/LAV envelope in syncytium formation and cytopathicity

Acquired immune deficiency syndrome (AIDS) is characterized by marked depletion of the T4+ helper subset of T cells. The aetiological agent of the disease, the human T-lymphotropic virus type III (HTLV-III)/lymphadenopathy-associated virus (LAV), specifically kills T4+ cells in vitro. Part of this specificity for the T4+ population residues in the relative efficiency with which HTLV-III infects these cells, as a result of a specific interaction between the T4 molecule and the virus envelope glycoprotein. In addition, the cytotoxic consequences of HTLV-III replication are dependent on cell type, as certain lymphoid and myeloid cells can be productively infected without notable cytopathic effect. Here we investigate the basis for the specific cytotoxicity of the virus, and report that high-level expression of the HTLV-III envelope gene induces syncytia and concomitant cell death in T4+ cell lines but not in a B-lymphocyte line. Syncytium formation depends on the interaction of envelope-expressing cells with neighbouring cells bearing surface T4 molecules. These results explain, at least in part, the specific cytopathic effect of HTLV-III infections.     

No evidence for thick deposits of ice at the lunar south pole

Shackleton crater at the Moon's south pole has been suggested as a possible site of concentrated deposits of water ice, on the basis of modelling of bi-static radar polarization properties and interpretations of earlier Earth-based radar images. This suggestion, and parallel assumptions about other topographic cold traps, is a significant element in planning for future lunar landings. Hydrogen enhancements have been identified in the polar regions, but these data do not identify the host species or its local distribution. The earlier Earth-based radar data lack the resolution and coverage for detailed studies of the relationship between radar scattering properties, cold traps in permanently shadowed areas, and local terrain features such as the walls and ejecta of small craters. Here we present new 20-m resolution, 13-cm-wavelength radar images that show no evidence for concentrated deposits of water ice in Shackleton crater or elsewhere at the south pole. The polarization properties normally associated with reflections from icy surfaces in the Solar System were found at all the observed latitudes and are strongly correlated with the rock-strewn walls and ejecta of young craters, including the inner wall of Shackleton. There is no correlation between the polarization properties and the degree of solar illumination. If the hydrogen enhancement observed by the Lunar Prospector orbiter indicates the presence of water ice, then our data are consistent with the ice being present only as disseminated grains in the lunar regolith.     

A role for Gbx2 in repression of Otx2 and positioning the mid/hindbrain organizer.

The mid/hindbrain (MHB) junction can act as an organizer to direct the development of the midbrain and anterior hindbrain. In mice, Otx2 is expressed in the forebrain and midbrain and Gbx2 is expressed in the anterior hindbrain, with a shared border at the level of the MHB organizer. Here we show that, in Gbx2-/- mutants, the earliest phenotype is a posterior expansion of the Otx2 domain during early somite stages. Furthermore, organizer genes are expressed at the shifted Otx2 border, but not in a normal spatial relationship. To test whether Gbx2 is sufficient to position the MHB organizer, we transiently expressed Gbx2 in the caudal Otx2 domain and found that the Otx2 caudal border was indeed shifted rostrally and a normal appearing organizer formed at this new Otx2 border. Transgenic embryos then showed an expanded hindbrain and a reduced midbrain at embryonic day 9.5-10. We propose that formation of a normal MHB organizer depends on a sharp Otx2 caudal border and that Gbx2 is required to position and sharpen this border.     

Variations in Variation and Selection: The Ubiquity of the Variation-and-Selective-Retention Ratchet in Emergent Organizational Complexity

The variation and selection form of explanationcan be prescinded from the evolutionary biologyhome ground in which it was discovered and forwhich it has been most developed. When this isdone, variation and selection explanations arefound to have potential application to a widerange of phenomena, far beyond the classicalbiological ground and the contemporaryextensions into epistemological domains. Itappears as the form of explanation most suitedto phenomena of fit. It is also found toparticipate in multiple interestingrelationships with other forms of explanation. We proceed with an examination of multiplekinds of phenomena, interrelationships withother members of the family of forms ofexplanation, and some novel applications evenwithin the home ground of evolutionary biology.     

A transgene containing lacZ inserted into the dystonia locus is expressed in neural tube

The site of integration of transgenes in the host genome can affect levels of expression and occasionally confer ectopic patterns of expression on otherwise tissue-specific genes. We describe here a line of mice in which an hsp68-lacZ transgene is expressed in unstressed developing neural tissue and where the transgene insertion has caused a mutation of a neural tissue-specific gene, dystonia musculorum (dt). This coincidence suggests that expression of the hsp68-lacZ construct may be controlled directly by cis-acting regulatory sequences that normally control the developmental expression of the dt gene. Such constructs may serve as useful tools for identifying new tissue-specific enhancers and their associated genes.     

Short-term effects of logging on red-backed voles and deer mice

Clearcutting and selective logging effects on red-backed voles ( Clethrionomys gapperi ) and deer mice ( Peromyscus maniculatus ) were studied (September–November, 1975; June–October, 1976) in Bridger-Teton National Forest, Wyoming. Five selective cuts (total 137 ha) removed 57 percent (range 34–74 percent) of the trees. One clearcut (9.6 ha) eliminate 84 percent of the trees. Soils remained mesic in selective cuts, but became xeric in the clearcut. Snap-trapping indicated that voles were most abundant on the unlogged and selectively cut mesic sites (76 percent of 408 captures), whereas deer mice were more common on the xeric clearcut (80 percent of 60 captures). Species composition remained unchanged on selective cuts following logging (77 percent voles of 256 captures), but changed from predominantly voles to mostly deer mice (80 percent of 60 captures) in the clearcut. Intraspecific age and sex ratios, litter sizes, and morphological measurements were compared between logged and unlogged areas.