金黄色葡萄球菌核酸酶及其突变体酶动力学性质的比较

将金黄色葡萄球菌核酸酶的类似物及其不同点突变体的基因进行表达，经Ｂｉｏ－Ｒｅｘ７０柱层析及尿处理进行分离纯化，在ＳＤＳ－ＰＡＧＥ上显示一条蛋白带，相对分子质量约为１７ｋＤ。测试了它们的比活力，Ｋｍ，Ｖｍａｘ，Ｋｉ等酶动力学参数。结果表明，ＳＮａｓｅＲ与ＳＮａｓｅ的催化活性无显著差异，但ＳＮａｓｅＲ对底物的亲和力明显增加。     

配气凸轮优化设计综述

探讨了发动机配气凸轮设计的近代技术发展，重点论述了凸轮型线的静态优化设计和动态优化设计及系统优化设计，阐述了这三种方法的特点以及用这些方法设计凸轮的优缺点，在此基础上讨论了凸轮型线优化设计方法的发展方向．     

大型电站汽轮发电机板翅式空-空冷却器优化设计

研究了大型电站汽轮发电机板翅式空ˉ空冷却器最优设计问题；建立了以最小质量为目标函数的最优设计数学模型，采用复形调优法编制了考虑工作条件、几何约束、热力参数约束的不同换热翅型的优化设计软件包，为扩大大型电站汽轮发电机的使用环境提供了条件。经比较分析，在给定约束条件下，锯齿形型面为最佳，其优化设计结果质量可减少约１６％。     

各种排样方式下顶点算法的研究

多边形顶点算法是冲裁排样优化问题的一种新算法。本文在单排顶点算法的基础上，对其它常用排样方式进行了研究，通过对原图形数组的适当拆分和重组构成新的多边形，即可采用在图形内求多边形顶点处宽度的算法。该算法不仅简化了计算，提高了运算速度，而且保证了最优解。     

刚性有限元与有限元耦合求解方法

提出刚性有限元（RFEM）与有限元（FEM）耦合求解方法，从能量原理导出了耦合矩阵方程，通过极限分析算例说明了该方法的可行性及有效性。     

非确定性 TS 模糊神经模型鲁棒性的统计判据

非确定性情况下的鲁棒性是智能控制系统不可避免的一个问题．本文将ＴＳ模型的模糊神经网络表达加以拓展，提出并证明了一种基于信息论统计测度的鲁棒性描述判据，该判据是由模糊神经网络、子波变换和信息论统计测度３个部分组成，着重体现了神经信号处理、可变分辨率和随机信号处理的综合，这就为分析智能控制系统的鲁棒性提供了一种有效的数学形式．该结论有助于非确定性智能控制系统的建模与智能控制器的设计．     

Electrochemical behavior of a series of azobenzene self-assembled monolayers on gold

Conclusion This work shows that the structures of C_n AzoC₂ (n = 1, 2, 3, 4) SAMs are significantly different and influence the observed electrochemical behavior of these novel azobenzene systems. The sluggish electron-transfer kinetics of azobenzene SAMs were explained by the alkyl spacer between azobenzene and electrode, the spatial inhibition for structural change in the redox process of azobenzene, as well as the ion-transportation barriers. For decrease of the apparent electrontransfer rate constants with increasing the length of end-groups, and the electrochemical inaccessibility of C₄AzoC₂ SAMs, the spatial inhibition for structure conformational change and ion-transportation was considered as the predominant factor.     

Organic solvents enhanced spectrofluorimetric method for determination of laccase activity

A novel fluorimetric method for determination of laccase activity in organic solvents is proposed, based on the oxidation ofo-phenylenediamine (1,2-diaminobenzene, OPDA) catalyzed by laccase yielding 2,3-diaminophenazine. The optimal conditions for laccase in organic media areT=55°C, pH=6.5, 1.0×10⁻²mol/L OPDA, 1.25 mL ethanol, 1.25 mL 1,4-dioxane and 1.25 mL acetone. The linear range of the method proposed in ethanol, 1,4-dioxane and acetone media were 0.44–19.33, 0.11–20.85, 0.38–21.05 U with the detection limit of 0.088, 0.022, 0.076 U, respectively. The proposed method has been applied to the analysis of laccase activity of real samples with more accurate and sensitive than that of the previous method reported. Supported by the National Natural Science Foundation of China and the Special Founds of State Education Committee for Doctorate Research Huang Zuyun: born in 1963, Ph.D     

The dissociation of nickel-oxine in surfactant micelles

The dissociation kinetics of Ni complex (NiL₂) of Oxine (HL) by H⁺ was investigated in neutral (Brij-35), cationic (CTAN), and anionic (SDS) micelles. The kinetic results have been interpreted by using the distribution model. The relevant distribution constants were determined independently by spectrophotometry. Supported by Natidnal Science Foundation of China Cai Ruxiu: born in Nov. 1939, Professor     

关于虚位移独立性的研究

从虚位移方程的基础解系出发探讨虚位移的独立性，并根据Четаев定义将其结果推广到高阶非完整系统。同时，根据对实位移的相应讨论，明确指出实位移是不相互独立的。