Modeling studies on the changes of the Subtropical High over the Western Pacific during the last 100 years

Intensity index series of Subtropical High over the Western Pacific was constructed according to modeling results by using of CCM3.6 atmospheric model with climatologically SST forcing for 30 years, and with observed SST forcing from 1900 to 2000. The index series constructed by modeling closely correlated to the observations for 1951-1999 (c.c. 0.72) and to the statistically reconstructed one for 1900-1950 (c.c. 0.51). The index series for 1900-1999 showed significant 80 a and 40 a periodicity. A minimum of persistence in seasonal variations of the index occurred in Autumn, which was called "Autumn Barrier". It may relate to the "Spring Barrier", found in the seasonal variations of SST over the Eastern Equatorial Pacific. The increasing trend shown in the index series for the 20th century may associate with the global warming caused by enhancement of greenhouse effect.     

Construction of multivalent DNA vaccines forMycobacterium tuberculosis and its immunogenicity

The coding regions of Ag85B MPT-64, and ESAT-6 secreted proteins were cloned initially into the eukaryotic expression vector pJW4303, then transformed to E. coli Top 10 strain for plasmid DNA extraction and further analysis. Plasmids containing the right insertion were sequenced to confirm their identity. COS7 cells were transfected with a mixture containing serially diluted plasmid DNA encoding three secreted proteins and Lipofectin (Gibco). The supernatants and pellets prepared from various cell lines were run on SDS-PAGE gel and the expression of these proteins in COS7 cells were demonstrated by immunoblot using polyclonal or monoclonal antiserum of M.TBH37Rv. 21 days after first vaccination of C57BL-6 mice by all three recombinant eukaryotic expressing vectors, antibody titer for Ag85B reached 1∶3200. 21 days after second vaccination, the antibody titer reached 1∶102400. The highest antibody levels induced by multivalent vaccines after the second injection were equal to or even greater than the highest antibody levels of single DNA vaccine reported in literature after third injections. Antibody titer of MPT-64 was 1∶50 after the first injection and it reached 1∶200 after the second injection. No antigen-specific antibody against ESAT-6 was detected in sera harvested from immunized mice 21 days after both injections. Antigen-specific IFN-g level of Ag85B was 110 pg/mL while no antigen-specific IFN- g level of ESAT-6 and MPT-64 was detected even after third injections. To our knowledge, it is the first time that studies of polyvalent recombinant DNA vaccines against TB were carried out in C57BL-6 mice. Our results indicated that multiple DNA vaccines could be used to enhance protective responses against M.TB.     

Assessment of blood and oxygen delivery to flaps of rhesus using near infrared steady-state spectroscopy

Near infrared spectroscopy has been considered as a potentially ideal noninvasive technique for the postoperative monitoring of plastic surgery. In this study, 6 flaps were raised on rhesus monkeys' forearms. Blood and oxygen delivery to these flaps was monitored following vascular occlusions and inhalation of pure oxygen. Optical fibers were adopted in the probe of the oximeter so that the cutaneous detection could be performed in reflectance mode. Different and repeatable patterns of changes were measured following vascular occlusions on flaps. It is clear that the near infrared spectroscopy is capable of postoperatively monitoring vascular problems in flaps, especially the venous problem. Near infrared spectroscopy showed high sensitivity to detect the dynamic changes in flaps induced by inhalation of pure oxygen in this study. The experimental results indicated that it was potential to assess tissue viability utilizing the dynamic changes induced by some noninvasive stimulation (such as inhaling pure oxygen). It may be a new assessing method that is rapid, little influenced by other factors and less inconvenient to patients.     

PFBC-CC发电技术的进展及创新发展

介绍和分析了增压流化床联合循环（PFBC－CC）发电技术在国外的进展以及国内由东南大学热能工程研究所作为技术依托单位承担的国家重点科技攻关项目－－贾汪PFBC－CC中试电站的研究开发、建设和成功试验运行情况，并在此基础上探讨提出了发展部分气化循环床燃烧联合循环（PGCFB－CC）的创新发展思路。     

异形坯结晶器三维流场模型的研究

依照湍动能理论及异形坯连铸的特点 ,建立了异形坯结晶器三维流场数学模型 ,运用该模型可分析不同水口参数及不同工艺参数条件下的流场分布 ,从而对连铸过程进行离线分析 ,确定最佳参数 ,并可作为在线控制模型的基础     

脉冲电晕技术降低柴油机微粒排放的实验研究

利用线－筒式荷电装置和高压窄脉冲电源来降低柴油机的微粒排放，分析了不同电源电压和脉冲频率下排气微粒的脱除效率，并在不同转速和负荷下进行了实验研究。研究结果表明，电源电压和脉冲频率是影响微粒脱除效率的两个主要因素，其中电源电压是最主要的影响因素，利用脉冲电晕放电降低柴油机的微粒排放效率明显的，是一种很有发展前途的控制尾气排放的新技术。     

偏最小二乘--荧光光度法同时测定多种酚体系

酚类化合物具有平面共轭的分子结构,因而具有较强的内源荧光。在苯酚含量为0～1×10~(-4)mol·L~(-1),对甲基酚含量在0～1×10~(-4)mol·L~(-1),邻苯二酚含量在0～5×10~(-5)mol·L~(-1)时线性关系较好.但是在线性范围内3种酚的荧光强度并不具有良好的线性加和性,而且苯酚、对甲酚、邻苯二酚的荧光光谱严重重叠,常规分析方法难以实现多组分同时测定。文中采用了偏最小二乘法(PLS)处理该3组分的荧光光谱数据,实现了对模拟样品中3组分的同时测定。     

长江上游生态修复与重点区域治理研究

在分析水土流失对洪水、河道影响与洪灾形成的基础上，通过比较分析与实地考察，阐述了四川省域水土保持在长江上游生态建设中的地位。指出了长江上生态修复的重点区域，并就治理对策作了分析探讨。     

LD泵浦的掺Yb3+双包层光纤激光器的实验研究

采用国产的掺Yb^3 双包层光纤和包层泵浦技术，分别对10m和20m光纤的输出特性进行了研究。10m光纤在1064nm获得58mW的激光输出，阈值为138.8mW，斜效率为10％；20m光纤在1067nm获得104.8mW的激光输出，阈值为109mW。     

玻璃纤维增强塑料加固砼梁的抗剪试验

通过对15根玻璃增强塑料（GFRP）片材加固混凝土梁的抗剪试验，研究GFRP片材的形状、加固方式、加固率、以及梁的剪跨比对加固梁抗剪承载力和破坏模式的影响。文中采用拱形桁架模型解释GFRP加固梁的剪切破坏机理，提出GFRP片材加固钢筋混凝土梁的抗剪承载力的计算公式。