A transgenic insertion upstream of sox9 is associated with dominant XX sex reversal in the mouse

In most mammals, male development is triggered by the transient expression of the Y-chromosome gene, Sry, which initiates a cascade of gene interactions ultimately leading to the formation of a testis from the indifferent fetal gonad. Several genes, in particular Sox9, have a crucial role in this pathway. Despite this, the direct downstream targets of Sry and the nature of the pathway itself remain to be clearly established. We report here a new dominant insertional mutation, Odsex (Ods), in which XX mice carrying a 150-kb deletion (approximately 1 Mb upstream of Sox9) develop as sterile XX males lacking Sry. During embryogenesis, wild-type XX fetal gonads downregulate Sox9 expression, whereas XY and XX Ods/+ fetal gonads upregulate and maintain its expression. We propose that Ods has removed a long-range, gonad-specific regulatory element that mediates the repression of Sox9 expression in XX fetal gonads. This repression would normally be antagonized by Sry protein in XY embryos. Our data are consistent with Sox9 being a direct downstream target of Sry and provide genetic evidence to support a general repressor model of sex determination in mammals.     

酚与4,4′-联吡啶π-π叠加作用的核磁氢谱研究

通过核磁氢谱法研究酚类化合物与4,4′-联吡啶季铵盐π-π叠加作用,研究了吡啶环上α-H,β-H及氧乙基上的α′-H,β′-H的化学位移值及其改变的情况.结果表明,β-萘酚、α-萘酚、对苯二酚、间苯二酚及苯酚使上述氢的化学位移降低,其化学位移降低次序为:β萘酚>α-萘酚>对苯二酚、间苯二酚>苯酚.而8-羟基喹啉和间硝基苯酚导致上述氢的化学位移稍升高.     

灌孔混凝土砌块砌体受压性能研究

通过对灌孔混凝土砌块砌体中混凝土芯柱三向受压应力状态进行分析,提出了适用于灌孔混凝土受压弹性模量的计算方法.同时,基于大量的试验结果,提出了适用于灌孔混凝土砌块砌体的泊松比、峰值应变、极限应变等参数,并引入到Domingo J.Carreira等提出的混凝土本构模型中,得出了适用于灌孔混凝土砌块砌体受压的应力-应变全曲线公式.对比结果表明,计算值与试验结果吻合良好,可供灌孔混凝土砌块砌体结构设计和研究参考.     

Nb2O5掺杂及TiO2压敏陶瓷埋烧工艺的研究

通过微结构分析、I-V特性及复阻抗频谱的测量,比较了埋烧和传统的裸烧工艺对于Nb^5 掺杂的TiO2压敏陶瓷材料的压敏电压和非线性系数的影响,结果表明掩埋法烧结可以降低该类陶瓷材料的压敏电压和非线性系数;考察了Nb2O5掺杂的作用,表明Nb^5 固溶于TiO2中取代Ti^4 使晶粒半导化．Nb2O5掺杂量对TiO2压敏陶瓷的I-V特性和微观结构都会有影响作用,适量Nb^5 的掺杂有助于晶粒的生长.     

一种地面目标的相关跟踪方法

提出了一种用多子模板对目标进行相关跟踪的方法，该方法建立了综合多子模板相关结果、目标跟踪判断及自适应刷新模板等准则，较好地解决了相关跟踪中如何判断目标锁定、部分遮挡、相似目标出现及模板的刷新等问题。