RhoA/Rho激酶在大鼠阴茎勃起机制中的调节作用

一般认为一氧化氮（nitric oxide,NO）释放增加促进小动脉和海绵体平滑肌舒张是导致男性阴茎勃起的主要机制。研究发现内源性血管收缩因子对维持阴茎萎状态有重要作用。应用大鼠模型活体检测使用Y-27632拮抗Rho-激酶活性后对阴茎勃起生理机制的影响;应用免疫转印技术检测 阴茎海绵体内RhoA和Rho激酶蛋白的表达。结果显示在大鼠海绵体组织内有内源性RhoA和Rho激酶蛋白的表达和存在;Y-27632海绵体内注射阻滞RhoA/Rho激酶活性使ICP和CCP/MAP比值明显升高;局部小剂量应用Y-27632对MAP没有明显影响;Y-27632可增强系列电刺激引起的由NO介导的CCP/MAP比值的增加;NO合成酶和鸟苷酸环化酶抑制剂的作用不能阻滞RhoA/Rho激酶抑制剂对大鼠阴茎海绵体平滑肌的松弛作用和CCP/MAP的增加。说明RhoA/Rho激酶信号系统发挥了维持海绵体萎软状态重要作用,这是与NO介导途径不同的阴茎勃起生理调节机制。RhoA/Rho激酶抑制剂可能是ED治疗的新领域新方法。     

Application of microfluidic chip to realize controllable pH gradient

A kind of PH gradient microfluidic chips through soft-lithography microfabrication for isoelectric focusing (IEF) and high performance liquid chromatography (HPLC) is introduced here. These pH gradient chips have the advantages such as easy fabrication, controllable pH range and precision, isoelectric focusing and separation at the same time, low voltage for isoelectric focusing, and time stable pH gradient. This method has potential application to sample preparation, separation and analysis of microfluidic chips.     

光突发交换网络的数据信道分组抢占调度策略研究

光突发交换是面向下一代因特网的光交换模式,它结合了光电路交换和光分组交换的优点,同时克服了他们的一些缺点.介绍了光突发交换网络中一种支持区分服务的数据信道调度策略,通过仿真分析了数据信道分组策略对网络性能的影响,为进一步研究提供了有价值的参考.     

半导体热电材料Bi1-xSbx薄膜的电化学制备

B I1-XSBX半导体合金是性能优异的热电和磁电功能材料,为制备固态电制冷器件、温差发电器件和磁电器件的重要材料。电化学沉积薄膜技术工艺设备简单成本低,在半导体薄膜制备方面有很好的应用前景。系统研究了高浓度盐酸(2.4 MOL/L)的B I和SB盐酸溶液,成份从纯铋逐步变化到纯锑的B I1-XSBX合金半导体薄膜电化学沉积特性。测试了沉积过程I-V循环曲线和和沉积电荷效率等电化学参数。结果表明在所有成份范围内都可以得到典型的B I1-XSBX固溶体结构的高质量薄膜。薄膜生长为典型的溶液扩散控制过程,具有高的沉积电荷效率。薄膜沉积和溶解之间的电位差随溶液中SB(Ⅲ)离子浓度增加而增大,生长的薄膜越来越稳定。在30%SB浓度附近,电化学过程、薄膜结构和性能发生明显的突变。应用X射线衍射和电子显微镜研究薄膜结构,发现薄膜具有明显的(012)择优取向,薄膜晶粒尺寸也随SB浓度的增加而变化。     

Microbial starch-binding domains are superior to granule-bound starch synthase I for anchoring luciferase to potato starch granules

Microbial starch-binding domains (SBD) and granule-bound starch synthase I (GBSSI) are proteins which are accumulated in potato starch granules. The efficiency of SBD and GBSSI for targeting active luciferase reporter proteins to granules during starch biosynthesis was compared. GBSSI or SBD sequences were fused to the N- or C-terminus of the luciferase (LUC) gene, via an artificial Pro-Thr encoding linker sequence. The genes were introduced into an amylose-free (amf) potato mutant. It appeared that SBD was superior to GBSSI as a targeting sequence, mainly because the luciferase retained higher activity in the SBD-containing fusion proteins than in the GBSSI-containing ones.     

宏观面内剪切作用下多尺度裂纹模型的耦合闭合解

裂纹在面内剪切荷载作用下,宏观应力场呈反对称分布,微观应力场将不再具有反对称性,可能是面内拉伸与面内剪切的混合型分布.另外,考虑微观裂纹前还有一个刃型位错区,据此建立起一个多尺度嵌套的裂纹模型,可以描述从原子尺度下的刃型位错区过渡到Ⅰ、Ⅱ复合型微观裂纹,再过渡到Ⅱ型宏观裂纹.材料不同尺度下的损伤,通过约束过渡区相连接,引入尺度变换因子的概念,并利用位移连续性条件与应力场匹配条件,求解出多尺度耦合问题的闭合形式的解.通过数值计算,讨论了材料宏观力学性能对不同尺度下各影响因素的的敏感性.研究结果表明,材料破坏机理非常复杂,与作用荷载、不同尺度下缺陷的几何形状、尺寸及材料性能等因素相关.     

Accelerated carbonation and leaching behavior of the slag from iron and steel making industry

Ground granulated blast furnace slag(GGBFS)and steelmaking slag have been used as a raw material for cement production or as an aggregate to make concrete,which contribute aluminum,calcium,iron,and silicon oxides.The suitability of the slag for a particular application depends on its reactivity,cost,availability,and its influence on the properties of the resulting concrete.For the interest of durability studying of concrete in the presence of slag,the accelerated carbonation products and leaching behavior of the slag and Portland cement(PC)were studied.The experimental results confirmed that the slag was more resistant to carbonation compared to PC.The carbonation degree of GGBFS reduced by 17.74%;and the carbonation degrees of steelmaking slags reduced by 9.51%-11.94%.Carbonation neutralized the alkaline nature of the hydrated pastes and gave rise to the redox potential of the leachate slightly(30-77 mV).The carbonation also increased the release of most of the elements presented,except for calcium,to the aqueous environment.It is concluded that blend cements(PC plus slag)have economical advantages and better durability compared to PC.     

温度和pH值对羊毛织物尺寸稳定性的影响

通过一系列实验分析了温度和pH值对羊毛纤维的膨胀和羊毛织物尺寸变化的影响．在pH2．1的溶液中，羊毛织物的尺寸随温度的升高而下降，变化的幅度取决于织物组织和织物的定型率．在羊毛等电点pH4．8时，纤维的膨胀达到最小值，而织物的尺寸则随着溶液酸性的增加而减小．