Arousal and fright responses and their habituation in the slippery dick,Halichoeres bivittatus

Summary Behavioral arousal and fright responses ofHalichoeres bivittatus occurred in aquaria to a moving shadow and a tap stimulus. Arousal was characterized by changes in the beat of pectoral fins, dorsal fin erection and eye movements, whereas in fright, adduction of pectoral and dorsal fins and rapid forward movement occurred. Serial stimulus presentation caused the fright response to be replaced by arousal which habituated in that the proportion of behavioral components exhibited decreased during the process.P.R. Laming was in receipt of a Royal Society travel grant for assistance in the expenses of this work, for which he is duly grateful. We are grateful to Nick Zinkowski, Culebra for the loan of minnow traps.     

Evaluation of monoaminergic receptors in the genetically epilepsy prone rat

Summary The intensity of sound-induced convulsions in the genetically epilepsy-prone rat (GEPR) was reduced in a dose related fashion by intracerebroventricular administration of dobutamine, (₁ agonist), terbutaline (₂ agonist) or phenylephrine (₁ agonist). BHT-920 (₂ agonist) did not cause a dose-related decrease in sound-induced convulsion intensity. Binding studies showed that whole brain and receptor densities (B_max) were normal while the K_d was increased for the ligand in GEPR brain.Acknowledgment. We are most grateful to Boehringer Ingelheim for generously supplying BHT 920. We are also indebted to Ciba-Geigy Corporation for the gift of terbutaline hydrochloride and phentolamine hydrochloride. The work was supported in part by NIH grant NS 16829.     

Physiological state of submitochondrial particles and their susceptibility to Triton X-100

Summary The solubilizing effect of Triton X-100 on beef heart submitochondrial particles (ETP_H) has been studied under various physiological conditions. Coupled, uncoupled and azide-inhibited ETP_H particles have been studied. Quantitative and qualitative differences are found in the proteins solubilized by the detergent from ETP_H particles under the various conditions tested.Acknowledgments: This work was supported in part by a grant from a Spanish Comisión Asesora para la Investigación Cientifica y Técnica. M.C. Barbero was recipient of a scholarship from the Basque Government.     

Excretion of acid hydrolases during molting inPhilosamia ricini

Summary The level of the acid hydrolases -glucuronidase (EC 3.2.3.11), acid phosphatase (EC 3.1.3.2) and acid protease (EC 3.4.4) was studied during larval growth and molting inP. ricini. The level of activity of these enzymes remained low during larval growth; however, the level increased sharply at the time of molting and declined sharply thereafter in the newly ecdysed insect. Interestingly, the diminished activity of these enzymes was almost quantitatively recovered in the cast-off cuticle. The excretion of acid hydrolases through the cast-off cuticle has hitherto not been reported in insects during molting.     

‘Chloride-cell’ — like mitochondria-rich cells of salamander larva gill epithelium

Summary Two types of mitochondria-rich cells (MRC) are described ultrastructurally in the gill epithelium of salamander larva. They resemble MRC found in larval ventral epidermis. Histochemical localization of carbonic anhydrase indicated numerous positive reacting cells, most of them flask-shaped. Morphological and functional similarities to fish chloride cells are discussed.     

A new interstellar molecule: tricarbon monoxide

The cold dark interstellar Taurus Molecular Cloud One (TMC-1) is a rich source of acetylenic and polyacetylenic molecular species. As well as linear closed-shell molecules (H(C triple bond C)nCN) and symmetric rotors (CH3C triple bond CH, CH3C triple bond CCN), several radicals (C triple bond CH, C triple bond CCN, (C triple bond C2H) have also been identified, many of which had not been studied previously in the laboratory. Whether the observed abundances can be understood in terms of purely gas-phase ion-molecule chemical schemes, which produce reasonable agreement for the simplest polyatomic species, is unclear; alternative models involving the particulate interstellar grains as catalysts or sources have also been suggested. We now report the detection in TMC-1 of a new molecule, tricarbon monoxide (C3O), whose pure rotational spectrum has only very recently been studied in the laboratory. As C3O is the first known interstellar carbon chain molecule to contain oxygen, its existence places an important new constraint on chemical schemes for cold interstellar clouds. In fact, the observed abundance of tricarbon monoxide fits quite well into our model of galactochemistry.     

Genetic basis of susceptibility for development of neoplasms following treatment with N-methyl-N-nitrosourea (MNU) or X-rays in the platyfish/swordtail system

Summary Specific genotypes of the xiphophorine fish develop neoplasms following treatment with N-methyl-N-nitrosourea or X-rays. Several of these neoplasms can be related to the presence of specific chromosomes. The implications of these findings are discussed.Supported by Deutsche Forschungsgemeinschaft through Sonderforschungsbereich 103 Zellenergetik und Zelldifferenzierung, Marburg (projects C 9 and C 10), and by Justus-Liebig-Universität Giessen. We are indebted to Prof. K. Frese, Veterinär-Pathologisches Institut, Giessen and Dr H. D. Mennel, Pathologisches Institut, Freiburg, for their help in the classification of neoplasms. We furthermore thank K. Klinke for breeding the fish. Dedicated to Prof. C. Kosswig on the occasion of his 75^th birthday.The paper contains parts of the dissertations of S. Abdo, J. Haas and G. Kollinger.supported by the Egypt ministry of education.     

Experimental immunity against trypanosomiasis

Summary 9 groups of 6 female rats were used in an experiment using fraction 3 ofTrypanosoma rhodesiense. 500 g gave 100% immunoprotection and 1000 and 1500 g gave 66% immunoprotection when challenged with 5×10² T. brucei. 2 groups of 10 female rats were tested for a short period inoculation immune response. In this, 750 g of fraction 3 ofT. rhodesiense gave 70% immunoprotection when challenged withT. brucei.I thank the University of Zambia for support; Dr M. A. Q. Awan, Mazabuka, Zambia, forT. rhodesiense andT. brucei; Prof. J. W. Kibukamusoke, Dr O. Okong'o and Dr W. W. Anokbonggo for advice; Mr L. Nyaliti for technical help.     

Chromosome variations inXenopsylla astia Rothschild, 1911 (Siphonaptera). A preliminary report

Summary 2 populations of rat fleas,Xenopsylla astia, collected from (A) Bombay and (B) Trivandrum, were found to differ in chromosome number as follows; (A) 2n=10 in both sexes; (B) 2n=18 (male) and 20 (female). Preliminary observations have further shown that the 2 populations differ in certain morphological characters and that their reciprocal hybrids are sterile in the male and partially sterile in the female sex.Acknowledgments. We wish to express our deepest gratitude to Dr Robert E. Lewis, Professor of Entomology, Iowa State University, USA, for confirming our identification of the 2 samples of rat fleas. We are indebted to Dr N.R. Prabhoo, Reader in Zoology, University of Kerala, Trivandrum, for his advice and helpful suggestions. We are also indebted to Prof. K.M. Alexander, for encouragement and providing facilities. Financial help of the University Grants Commission, India, to one of us (C.T.) is also gratefully acknowledged.