Impact of biofilm matrix components on interaction of commensal Escherichia coli with the gastrointestinal cell line HT-29

Commensal Escherichia coli form biofilms at body temperature by expressing the extracellular matrix components curli fimbriae and cellulose. The role of curli fimbriae and cellulose in the interaction of commensal E. coli with the intestinal epithelial cell line HT-29 was investigated. Expression of curli fimbriae by the typical commensal isolate E. coli TOB1 caused adherence and internalization of the bacteria and triggered IL-8 production in HT-29 cells. In particular, induction of IL-8 production was complex and involved curli-bound flagellin. While cellulose alone had no effect on the interaction of TOB1 with HT-29 cells, co-expression of cellulose with curli fimbriae decreased adherence to, internalization and IL-8 induction of HT-29 cells. Investigation of a panel of commensal isolates showed a partial correlation between expression of curli fimbriae and enhanced internalization and IL-8 production. In addition, a high immunostimulatory flagellin was identified. Thus, the consequences of expression of extracellular matrix components on commensal bacterial-host interactions are complex.     

Differentiated cells are more efficient than adult stem cells for cloning by somatic cell nuclear transfer

Since the creation of Dolly via somatic cell nuclear transfer (SCNT), more than a dozen species of mammals have been cloned using this technology. One hypothesis for the limited success of cloning via SCNT (1%-5%) is that the clones are likely to be derived from adult stem cells. Support for this hypothesis comes from the findings that the reproductive cloning efficiency for embryonic stem cells is five to ten times higher than that for somatic cells as donors and that cloned pups cannot be produced directly from cloned embryos derived from differentiated B and T cells or neuronal cells. The question remains as to whether SCNT-derived animal clones can be derived from truly differentiated somatic cells. We tested this hypothesis with mouse hematopoietic cells at different differentiation stages: hematopoietic stem cells, progenitor cells and granulocytes. We found that cloning efficiency increases over the differentiation hierarchy, and terminally differentiated postmitotic granulocytes yield cloned pups with the greatest cloning efficiency.     

中国大学生朗读英语基本句型的语调分析

通过使用Praat语音分析软件，对我国大学生和以英语为母语的本族语者所读的英语基本句型的语调进行了对比和初步的分析，指出了我国大学生朗读英语常用基本句型时所使用的语调的大致形态和特点，揭示了其中存在的问题。希望对我国大学生英语语调的学习有所启示和帮助。     

一般非线性系统的鲁棒适应控制

主要对结构不确定性中含有未知参数和未知非线性函数的非线性系统,在参数上界未知的情况下,对参数进行在线估计,设计出适应控制器.     

中部地区中小学教师教育公平意识的调查与研究

本研究采用自编问卷对中部六省的600名中小学教师的教育公平意识进行了问卷调查,调查的内容包括教师对学生的关注、对学生的评价、对学生进行教育资源的分配和教育公平感等四个维度.调查结果显示,不同性别、不同学历、不同教龄,不同学段和不同区域的教师在教育公平意识上存在一些差异.这些差异在一定程度上表现了当前中部地区中小学教师教育公平意识状况,并有其内在的原因.     

九龙江特大桥钢吊箱设计施工

随着高速公路、铁路建设的飞速发展,大跨径深水桥梁基础多采用群桩基础.大体积砼承台.水中承台施工与钢吊箱围堰的设计施工密不可分,如何在保证钢吊箱围堰施工质量、安全的情况下让结构更为简单,将工程成本降至最低,文章根据以往施工的经验,结合九龙江特大桥钢吊围堰施工实际,详细介绍了钢吊箱围堰设计施工.     

钼-硫氰酸盐-甲基紫高灵敏离子缔合体系的研究和应用

本文研究了在聚乙烯醇存在下,钼(Ⅵ)—SCN—甲基紫高灵敏离子缔合体系,在0·10—0·14M硫酸介质中,该体系λ_(max)=530nm,表观摩尔吸光系数_(530)=9·20×10~5l·mol~(-1)·cm~(-1),0～μgM_0/25ml符合比尔定律,选择性较好,并用于钢铁中钼的测定。对显色反应机理作了初步探讨。