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951.
952.
E. Bonilla M. Salazar J. Estevez H. Hernández P. Rangel 《Cellular and molecular life sciences : CMLS》1984,40(8):868-869
Summary After i.p. inoculation with the Guajira strain of Venezuelan equine encephalomyelitis virus a significant decrease in the density of (3H) spiroperidol binding sites in the striatum, midbrain and frontal cortex was observed. No changes in the affinity of the receptors could be demonstrated. This finding is compatible with neuronal degeneration caused by the viral infection.Acknowledgments. This work was partially supported by Condes-Luz and Fundacite-Zulia. Butaclamol isomers were a gift of Ayerst Laboratories, Montreal, Canada. 相似文献
953.
Comparative distribution of vasoactive intestinal polypeptide (VIP), substance P and PHI in the enteric sphincters of the cat 总被引:2,自引:0,他引:2
G P McGregor A E Bishop M A Blank N D Christofides Y Yiangou J M Polak S R Bloom 《Experientia》1984,40(5):469-471
In the feline gastrointestinal tract, the neuropeptides, substance P, VIP and PHI were investigated by specific radioimmunoassays and immunocytochemistry. The concentrations of all 3 peptides and the level of peptidergic innervation were significantly less in the anal sphincter than elsewhere, whereas no significant differences were seen between other sphincter and non-sphincter regions. 相似文献
954.
955.
956.
Human hepatitis B vaccine from recombinant yeast 总被引:22,自引:0,他引:22
W J McAleer E B Buynak R Z Maigetter D E Wampler W J Miller M R Hilleman 《Nature》1984,307(5947):178-180
The worldwide importance of human hepatitis B virus infection and the toll it takes in chronic liver disease, cirrhosis and hepatocarcinoma, make it imperative that a vaccine be developed for worldwide application. Human hepatitis B vaccines are presently prepared using hepatitis B surface antigen (HBsAg) that is purified from the plasma of human carriers of hepatitis B virus infection. The preparation of hepatitis B vaccine from a human source is restricted by the available supply of infected human plasma and by the need to apply stringent processes that purify the antigen and render it free of infectious hepatitis B virus and other possible living agents that might be present in the plasma. Joint efforts between our laboratories and those of Drs W. Rutter and B. Hall led to the preparation of vectors carrying the DNA sequence for HBsAg and antigen expression in the yeast Saccharomyces cerevisiae. Here we describe the development of hepatitis B vaccine of yeast cell origin. HBsAg of subtype adw was produced in recombinant yeast cell culture, and the purified antigen in alum formulation stimulated production of antibody in mice, grivet monkeys and chimpanzees. Vaccinated chimpanzees were totally protected when challenged intravenously with either homologous or heterologous subtype adr and ayw virus of human serum source. This is the first example of a vaccine produced from recombinant cells which is effective against a human viral infection. 相似文献
957.
Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte-macrophage colony stimulating factor 总被引:5,自引:0,他引:5
N M Gough J Gough D Metcalf A Kelso D Grail N A Nicola A W Burgess A R Dunn 《Nature》1984,309(5971):763-767
DNA clones specifying the murine granulocyte-macrophage colony stimulating factor have been isolated. This haematopoietic growth factor is encoded by a unique gene specifying a messenger RNA of 1,200 nucleotides and a polypeptide of 118 amino acids. It bears no structural similarity to the functionally related factor, interleukin-3, described recently. 相似文献
958.
959.
J. A. Ruth M. J. Gershten J. K. Disbrow 《Cellular and molecular life sciences : CMLS》1984,40(7):692-694
Summary The accumulation of3H-dopamine by synaptic vesicles from rat striatum was significantly stabilized in a membrane impermeant medium. The characteristics of dopamine accumulation by striatal vesicles were quite similar to those reported for dopamine accumulation by a whole brain vesicle preparation in the same medium, and were siginificantly different from the characteristics previously reported for vesicular accumulation of norepinephrine.Acknowledgments. This work was supported by grant NS 18752 (NIH) of the United States Public Health Service. Reprint requests to J. A. R. 相似文献
960.
Comparison of the survival, fecundity and offspring size of Glossina palpalis palpalis females fed reconstituted oven-dried blood, fresh, frozen/thawed, or reconstituted freeze-dried blood showed that oven-drying at 45 degrees C does not diminish the nutritional quality of blood. The significance of this finding is discussed with a view to optimizing costs and conditions of blood-diet storage and transportation in the context of mass-rearing of tsetse flies. 相似文献