Abnormal pattern detected in fragile-X patients by pulsed-field gel electrophoresis.

The fragile-X syndrome is the most frequent inherited form of mental retardation, with an incidence of 1 in 1,500 males. It is characterized by the presence of a fragile site at Xq27.3 induced in vitro by folate deprivation or by inhibitors of deoxynucleotide synthesis. Its mode of inheritance is unusual for an X-linked trait, with incomplete penetrance in both males and females. Some phenotypically normal males transmit the mutation to all their daughters who rarely express any symptoms, but penetrance is high in sons and daughters of these carrier women. Genetic and physical mapping of the Xq27-q28 region has confirmed that the disease locus is located at or very near the fragile site. Hypotheses proposed to account for the abnormalities in the inheritance of the disease include sequence rearrangements by meiotic recombination or a mutation that affects reactivation of an inactive X chromosome during differentiation of female germ cells. To detect such rearrangements, or methylation changes that may reflect a locally inactive X chromosome, we used pulsed-field gel analysis of DNA from fragile-X patients with probes close to the fragile-X locus. The probe Do33 (DXS465) detected abnormal patterns in fragile-X patients, but not in normal controls or in non-expressing male transmitters.     

产芽孢细菌B130（BACILLUS SPP）培养条件的初步研究

本文就产芽孢细菌B130(Bacillus spp.)培养条件进行了初步研究,筛选出产菌量较高且来源方便,价格低廉的培养基成分(％w/v):豆饼粉3,麸皮4,玉米粉3,最适pH:6.5或8.0,确定了最佳培养时间区间为平衡期末期。     

Erosion and Sediment Production in Small Watershed in Purple Hilly Areas and Prevention Techniques

Purple Soil distributes extensively and mainly in China. Because of abundant easily weatherable parent rocks/materials and unstable soil structure, and also influenced by parent materials, usage systems, and slope gradients, erosion and sediment production of purple soils are very severe with main fashions of water erosion and gravitational erosion. Basing on observed data in small watersheds, rainfall erosivity,vegetation coverage, previous soil water content, flow and relating factors such as climate, topograph of small watershed,land usage, and soil kinds are all the influence factors of erosion and erodibility of purple soil as well as sediment production and transport in small watershed of purple hilly areas.The effective technological countermeasures of ecosystem restoration, agricultural tillage for water conservation and erosion prevention, agriculture project, and soil changing for fertility and anti-erosion were provided.     

信息技术与学科教学的整合需要研究型教师

信息技术与学科的有效整合需要教师具备整合的基本能力，并成为研究型教师。指出教师要不断更新信息技术知识和技能，利用现代教学设施，依据教学实践开展研究，从而探索出有效的整合模式。     

Effect of 15(R)-15-methyl-prostaglandin E2 on iron absorption in the rat

Summary The administration of 15(R)-15-methyl prostaglandin E₂ (15(R)-15-M-PGE₂) in vivo significantly diminished the uptake of⁵⁹Fe into blood, spleen, liver, femur and dried intestine of rats, whereas acetylsalicylic acid (ASA) increased the counts significantly. This effect of ASA was counteracted by 15(R)-15-M-PGE₂. It is suggested that prostaglandins (PGs) might play an important role in inhibiting iron absorption at the intestinal level.This work was supported by grant No.6638 from CONICET (Argentina). The technical assistance of Mrs María E. Castro and Norma Rizzo is gratefully acknowleged.     

Energetic landscape of alpha-lytic protease optimizes longevity through kinetic stability.

During the evolution of proteins the pressure to optimize biological activity is moderated by a need for efficient folding. For most proteins, this is accomplished through spontaneous folding to a thermodynamically stable and active native state. However, in the extracellular bacterial alpha-lytic protease (alphaLP) these two processes have become decoupled. The native state of alphaLP is thermodynamically unstable, and when denatured, requires millennia (t1/2 approximately 1,800 years) to refold. Folding is made possible by an attached folding catalyst, the pro-region, which is degraded on completion of folding, leaving alphaLP trapped in its native state by a large kinetic unfolding barrier (t1/2 approximately 1.2 years). alphaLP faces two very different folding landscapes: one in the presence of the pro-region controlling folding, and one in its absence restricting unfolding. Here we demonstrate that this separation of folding and unfolding pathways has removed constraints placed on the folding of thermodynamically stable proteins, and allowed the evolution of a native state having markedly reduced dynamic fluctuations. This, in turn, has led to a significant extension of the functional lifetime of alphaLP by the optimal suppression of proteolytic sensitivity.     

Comparative study of the karyotypes of two Egyptian species of bats,Taphozous perforatus andTaphozous nudiventris (Chiroptera: Mammalia)

A detailed karyotypic analysis of two Egyptian species of bats,Taphozous perforatus andTaphozous nudiventris, was made on the basis of conventional data and G-band patterns. No detectable karyotypic difference was found between the two species (2n=42, F.N. 64). By comparison of G-band patterns, some differences between the two species were seen in the corresponding autosomal pairs. These results are reported for the first time in Egypt.     

新时期大学生成长观教育面临的主要问题

社会主义市场经济的建立和发展使人的主体性意识进一步增强,为大学生的发展提供了更加广阔的空间,对大学生的素质提出了更高的要求,也给教育工作者提出了新的要求.从个人才干增长与社会需要、奉献与索取、成长中的自由与必然三个方面阐述新时期大学生成长观教育中所面临的主要问题,以期在教育工作中做到有的放矢.     

在线固溶条件下的输出辊道受热分析

为满足300系列奥氏体不锈钢轧后在线目溶处理的要求，针对某厂已有设备条件，进行在线固溶处理输出辊道的改造，利用ANSYS有限元分析软件，通过对不同结构和材质条件下输出辊道的受热分析，为输出辊道的改造提供了理论依据。结果表明，将原有的铸铁辊道改为陶瓷辊道能够满足生产的要求。     

SIMULTANEOUS REACTION AND LIQUID-LIQUID EXTRACTION IN THE HYDROGEN PEROXIDE PRODUCTION

The gas-liquid-liquid reactive extraction system for preparing hydrogen peroxide via anthraquinone was investigated. The oxidation reaction of hydrogenated working solution was combined with the extraction of hydrogen peroxide from working solution in a sieve plate column. The reaction of 2-ethylanthrahydroquionone with oxygen and the liquid-liquid extraction of hydrogen peroxide take place simultaneously. The oxygen was introduced with hydrogenated working solution through a nozzle in the bottom of the column, which worked as agitated air as well as oxidation reagent. The results showed the oxidation and extraction do not hamper each other, on the contrary, the presence of oxidation gas in the column can promote the transfer of hydrogen peroxide from organic phase to aqueous phase, thus the reaction efficiency and extraction efficiency increased with increasing gas superficial velocity. Furthermore, the oxidation efficiency is almost 100% and the extraction efficiency is higher than 90% in this process.