国内亚健康研究现状综述

亚健康是21世纪人类面临的一个重要问题,目前国内研究尚处于起步阶段．本文采用文献资料、访谈等研究方法,从亚健康的概念、病因机制、症状及有效干预措施等四个方面进行了综述,并提出相关建议：对亚健康状态的评价不同于对疾病的评价,应以四维健康观为参照进行评价;应综合运用临床流行病学、卫生统计学、量表学、心理学、人文科学、系统生物学等各学科的研究方法,对亚健康进行系统研究;以具体人群作为研究对象,横向与纵向研究为突破口,确定不同人群亚健康状态的特征与分布,增强研究的针对性,从而为预防和治疗提供依据．     

基于矢量量化的仿生模式识别方法研究

该文探索了另一种新的仿生模式识别算法:基于矢量量化的仿生模式识别,用高维空间闭域作为同源类模板,并将其应用于语音关键词识别。实践表明仿生模式识别方法有许多优点,能够有效降低识别的错误率,识别效率优于传统模式识别方法。     

Single-cell discrimination based on optical tweezers Raman spectroscopy

The ability to discriminate between single cells in a label-free and noninvasive fashion is important for the classification of cells, and for the identification of similar cells from different origins. In this paper, we present the Raman spectroscopy-based identifi- cation of different types of single cells in aqueous media, and discrimination between the same types of cells from different donors using a novel Laser Tweezers Raman Spectroscopy (LTRS) technique, which combines laser trapping and micro-Raman spectroscopy. First, we measured the spectra of individual living human erythrocytes, i.e. red blood cells, and leucocytes (U937 cancer cells). High-quality Raman spectra with low fluorescence were obtained using a home-LTRS apparatus and 20 cells were measured for each cell type. The smoothing, baseline subtraction, and normalization of the data were followed by a principal components analysis (PCA). The PCA loading plots showed that the two different types of cells could be completely separated based only on the first component (PC1) (i.e. the peaks at 1300 cm1 ); the discrimination accuracy could therefore reach 100%. More than 50 spectra were taken for each erythrocyte obtained from the four healthy volunteers. The average discrimination accuracy was 84.5% for two random individuals taken from the four volunteers, according to the first and second PCs. This work demonstrates that LTRS is a powerful tool for the accurate identification and discrimination of single cells, and it has the potential to be applied for the highly sensitive identification of cells in clinical diagnosis and medical jurisprudence.     

细节决定成败 和谐促进发展——以烟台大学图书馆为例

简述了细节服务的内涵与重要性,介绍了一些因注重细节而获成功的事例,以烟台大学图书馆为例,讨论了高校图书馆文献流通部细节服务的具体体现,提出了一些行之有效的管理办法。     

表皮生长因子间接竞争酶联免疫吸附测定法的建立

目的：建立一种可应用于临床的快速定量测定表皮生长因子含量的酶联免疫吸附测定法(ELISA)。方法：以重组人表皮生长因子(ECF)为包被抗原和竞争抗原，两与一定量的ECF。抗血清反应，建立检测EGF的间接竞争ELISA方法。结果：理想的包被抗原质量浓度为1μg/mL，ECF抗血清的工作浓度为1：10000，酶标二抗工作浓度为1：3000，可测最适范围为0．5～16ng／mL，最小检测量为0．5ng/mL，批内和批间变异系数分别为6．21％和7．42％。得到回归方程y=-13．65ln(x) 81．554，相关系数R^2=0．997。结论：建立了快速定量检测ECF的间接竞争ELISA方法。     

鹅掌楸生殖生态研究:果实与种子变异格局

选择浙江龙王山自然保护区和贵州松桃两个居群的中国鹅掌啾,研究其果实与种子的变异格局。结果表明,两个鹅掌楸居群的生殖包装格局有显差别。分析翅果重、聚合果重、翅果长、聚合果长4个密切相关的指标,得出龙王山居群的频率分布属正态分市．而松桃居群的频率分布则偏离正态分布,并且是右偏的。这种差别意味着前具更多中等大小的果实和种子．而后以较大的果实和种子居多。就1个聚合果而言,饱满种子多位于果实中部,但资源却主要分布在果实中下部,     

General Improved KdV方程的三层加权平均线性差分格式

本文对广义Improved KdV方程的初边值问题进行了数值研究,提出了一个三层加权平均线性差分格式,分析了差分解的存在唯一性,证明了格式的二阶收敛性和稳定性.数值实验验证了差分格式的有效性.     

杂质对三元准周期超晶格隧穿性质的影响

应用δ函数势模型和散射矩阵方法,研究了势阱中的杂质对三元准周期超晶格电子隧穿性质的影响。结果表明,杂质对三元准周期超晶格的电子共振隧穿谱有明显影响。改变δ势垒高度会引起透射峰的移动;杂质放在势阱中的不同位置,也会使得电子透射率谱发生变化。     

社会资本视角下透析纽约三家社区图书馆给予的启示

借助2008年GongH等关于纽约三家社区图书馆的运行和发展情况调查所提供的材料,从社会资本角度,对美国社区图书馆的运行和特点进行了剖析,在此基础上,提出了我国社区图书馆建设应该采取的措施。     

Treatment of PC-3 cells with ultrasound combined with microbubbles induces distinct alterations in the expression of Bcl-2 and Bax

The objective of the present study was to investigate whether ultrasound combined with microbubbles induces apoptotic cell death in androgen-independent prostate cancer cells and to identify the probable mechanism. We used ultrasound in continuous wave mode with a frequency of 21 kHz and a spatial-average temporal-average intensity of 46 mW/cm². Ultrasound combined with microbubbles (200 μL SonoVue?) was used to treat androgen-independent human prostate cancer PC-3 cells for 30 s. PC-3 cells were divided into three groups: the control group, the ultrasound group and the ultrasound combined with microbubbles group. Immediately after treatment, trypan blue exclusion was used to assess cell viability. Cell apoptosis at 24 h after treatment was measured using transmission electron microscopy and flow cytometry. Western blotting was used to evaluate the expression of the apoptosis-related proteins, Bcl-2 and Bax. Ultrasound combined with microbubbles had a minimal effect on the viability of PC-3 cells and induced minimal levels of cell lysis. The level of apoptosis in PC-3 cells induced by this modality was significantly higher than in controls (12.77 ± 0.31% vs. 2.56 ± 0.22%, P<0.01). Treatment with ultrasound combined with microbubbles increased the expression of Bax, a pro-apoptotic protein, and decreased the expression of Bcl-2, an anti-apoptotic protein. It was concluded that ultrasound combined with microbubbles induces apoptotic cell death in human prostate cancer PC-3 cells through down-regulation of Bcl-2 and up-regulation of Bax.