用密度梯度离心分离的大白鼠胚胎海马细胞类型移植存活

在灭菌条件下,用机械解离法将15—20天的胚胎Wistar大白鼠的海马制成细胞悬浮液,并于4℃,用不连续的ficoll-urografin密度梯度离心法分离出不同的层次。大细胞在上层,小细胞在下层,分别加以收集、洗涤与再悬浮。5微升的每种细胞悬浮液分别注入4月龄Wistar雌性大白鼠的右侧海马,而同体积的生理盐水注入左侧作为对照。三周之后,对5只大细胞接受者与5只小细胞接受者作检查,每组各有2只动物见有移植物良好地存活。存活的大细胞主要是海马CA3区的大锥体细胞,此外还有乙酰胆碱酯酶阳性的大细胞,可能是齿状门CA4区的大多形细胞。存活的小细胞主要是乙酰胆碱酯酶阳性的小多形细胞与篮状细胞,此外还有形似齿状回的颗粒细胞和海马CA1区的小锥体细胞。根据本实验,只要作一些改进,被分类的移植细胞有可能在成鼠的脑内存活得更多更好。     

基于Simplex-annealing混合方法的模型参数估计

针对现有算法的导数依赖性及其局部优化性能 ,为控制工程中的模型参数估计课题提供一种新思路。把具有概率突跳特性模拟退火 (SA)和基于高维 Euclid空间中凸多面体结构的单纯形搜索法 (SM)有机地结合 ,通过对搜索操作和参数的有效设计 ,提出了一种基于 Sim plex- annealing混合算法 (SMSA)的模型参数估计方法。对以传递函数、状态空间和自回归滑动平均 (ARMA)模型形式表达的不同典型对象进行仿真 ,结果表明 :SMSA方法在模型结构已知的情况下可准确地估计参数 ,其性能明显优于单一遗传算法(GA)     

广义变系数KdV,mKdV方程的精确类孤子解

利用截断展开法和延拓齐次平衡法同时求出了广义变系数KdV方程和广义变系数mKdV方程的精确钟状类孤子解 .其基本思想是 :设方程的解形式为u(x ,t) =∑nm=0υm(t)Fm,　F =eα( ξ+ξ0 )1+eα( ξ+ξ0 )代入给定方程确定出n ,并令F的各次幂项的系数为零 ,得到超定可积分方程组 ,由此求出给定方程的精确类孤子解 .     

5-HMF抑制A375细胞增殖的信号转导通路

通过Caspase活性检测和Western blotting法对ROS介导的A375细胞凋亡和G0/G1细胞周期阻滞中相关蛋白进行分析。结果表明:5-HMF通过清除细胞内ROS激活了DNA损伤介导的P53磷酸化、AKT通路和MAPKs通路,这些通路共同作用于相应的下游底物,激活外源性的死亡受体通路和内源性的线粒体通路导致细胞的凋亡,其中,5-HMF通过上调线粒体中的促凋亡因子、下调抑凋亡因子并活化Bid而激活內源性途径;此外,P53、AKT等也可以抑制或增强G0/G1期主要调控因子的表达,导致G0/G1期阻滞,证明了5-HMF可抑制A375细胞增殖的信号转导通路。     

基于多重散射理论的二维声子晶体带结构计算

二维固体结构声子晶体如平板、板壳在结构材料的减振、降噪方面有广泛的应用,研究二维固体结构声子晶体的禁带特性,对减振、降噪结构材料及声学滤波器的设计有重要的理论指导意义.用声子晶体带结构计算的多重散射理论,计算了二维碳一环氧树脂声子晶体的能带结构.结果表明,将波速较大的碳柱体按正方形周期排列放入到波速较小的环氧树脂中,在布里渊区两个主要方向上出现了完全禁带.     

Flame spread over the surface of thermal insulation materials in different environments

Experiments were conducted in a plateau area in Lhasa and a plain area in Hefei China to investigate the flame spread characteristics on thermal insulation materials under different environmental conditions (pressure and oxygen concentration).Molded polystyrene foam (EPS) and extruded polystyrene foam (XPS) samples were placed horizontally on a small-scale flame spread experimental bench.Changes in the average length of the pool fire,flame spread speed,average flame height,and length of preheating zone were used to determine the effect of the plateau and plain environments on flame spread characteristics.These parameters were all larger in Hefei than in Lhasa,which indicates the fire hazard in Hefei will be higher than that in Lhasa if insulation materials of the same size are used.     

Carbon and hydrogen isotopic compositions and their evolutions of gases generated by herbaceous swamp peat at different thermal maturity stages

Coalbed methane (CBM) accumulation models include continuous gas accumulation and staged gas accumulation.However,studied on the geochemical characteristics and indices to evaluate staged accumulation CBM are lacking.This study for the first time obtained the carbon and hydrogen isotopic compositions of methane and ethane generated at different evolution stages using thermal simulation of samples prepared by treating an herbaceous swamp peat at different temperatures.The results showed that the carbon isotopic compositions of methane and ethane were obviously affected by the thermal evolution level of the starting sample,while the hydrogen isotopic compositions were closely related to the maturity of gases.The carbon and hydrogen isotopic compositions of gases generated by coal-forming organic matter with Ro values from 1.2%,1.7%,2.4%,3.2% and 3.7% to 5.2% were determinated.The relationship between Ro values and the carbon and hydrogen isotopic compositions of gases generated by coal-forming organic matter at different evolution stages as well as the carbon or hydrogen isotopic relationships between methane and ethane were established.The results provide a scientific basis for studying the genesis of CBM generated at different maturity intervals and understanding the geochemical characteristics of staged accumulation CBM.These results were applied to a case study on CBM from the southern Qinshui basin,and it was found that the CBM accumulated after the Middle Jurassic and was characteristic of staged gas accumulation.This is consistent with the result of geological studies,and further showed that the results of thermal simulation experiments are very important for evaluating the genesis of natural CBM.     

Accurate localization and excision of genomic islands in four strains of <Emphasis Type=Italic>Pseudomonas aeruginosa</Emphasis> and <Emphasis Type=Italic>Pseudomonas fluorescens</Emphasis>

Mobile genomic islands (GIs) can be excised from the chromosome, then form a circular intermediate and be reintegrated into the chromosome by the GI internal integrase. Some mobile GIs can also be transferred into a new receptor cell by transformation, conjugation, or transduction. The action sites of the integrase are usually flanked direct repeats (DRs) of the GIs. Accurate localization of the flanking sequences is a precondition for determining the mobility of the GI. Mobile GIs are generally associated with transfer RNAs (tRNAs). Based on the correlation between flanking sequences and tRNA sequences, the flanking sequences of 11 putative mobile GIs in Pseudomonas aeruginosa PAO1, P. aeruginosa PA14, P. fluorescens Pf-5 and P. fluorescens Pf0-1 were identified. Among the 11 GIs, Pf0-1GI-1 is responsible for benzoate degradation. PAO1GI-1, Pf5GI-2, Pf5GI-3, and Pf5GI-4 were confirmed experimentally to be excised from a chromosome to form a circular intermediate. The action sites of the integrases are these GIs direct repeats. Due to distinct DRs, cutting sites for the internal integrase of PAO1GI-1, Pf5GI-2, Pf5GI-3 and Pf5GI-4 were determined outside the T-loop of the tRNA^Gly gene, outside the anticodon loop of the tRNA^Ser gene and tRNA^Lys gene, and at the asymmetric 3′-end of the tRNA^Leu gene, respectively. PAO1GI-1 and other mobile GIs may be transferred into many different strains that belong to different phyla because of the clear flanking sequences. This study describes basic information about the action sites of the integrases, assesses the mobility of GIs, and can help design and transfer mobile GIs to candidate strains.     

Characteristics of strontium isotopes and their implications in the Qixing Cave of Guizhou,China

The strontium isotopic compositions (87Sr/86Sr) of samples including soils, bedrock, soil waters, drip waters and their corresponding speleothems in the Qixing Cave (QXC), Guizhou Province, China, were systemically measured and analyzed. The results indicate that there are significant Sr isotopic differences among samples. The mean 87Sr/86Sr ratios in drip water for the samples 1#, 4# and 9# were 0.709568, 0.709139 and 0.708761, respectively, which possibly result from different flow paths, residence times, and other hydrogeological processes in the unsaturated zone overlying QXC. Meanwhile, levels of 40.8%, 57.6% and 72.4% of Sr in drip waters for 1#, 4# and 9#, respectively, were derived from bedrock dissolution, which was calculated by the mixture model of the two end-members (soil and bedrock). There is, however, no positive correlation between the relative proportion from bedrock dissolution (δ13C value is 1.8‰) and drip water δ13CDIC values. The mean drip water δ13CDIC value in 1# is the heaviest (–4.5‰) with the lowest contribution rate of bedrock dissolution, whereas the value in 9# is the lightest (–9.3‰) with the highest contribution rate of bedrock dissolution. The proportion from host rock dissolution in 4# is higher than that in 1# and lower than that in 9#, while its mean drip water δ13CDIC value (–8.6‰) is higher than that of 9# and lower than that for 1#. This suggests that the prior calcite precipitation (PCP) processes in the unsaturated zone overlying the cave are responsible for the δ13CDIC value differences between different drip waters, and not bedrock dissolution. Furthermore, this study also demonstrates that the 87Sr/86Sr ratios of speleothems in the 1# and 4# mainly reflect the variation in the relative proportions from the soil system (soil water) and bedrock dissolution overlying the cave. It is, therefore, feasible to use the strontium isotopic signals of speleothems as an indicator for soil chemical weathering intensity, and consequently as a monsoon proxy in the study area.     

Effect of hydrogen donating ability of benzofuranone on the antioxidant activity

The rate constants for the hydrogen donation of three 3-aryl-benzofuranone compounds with tert-butoxyl radicals have been cal-culated through laser flash photolysis (LFP) technique, which were used to evaluate the hydrogen donating abilities of those 3-aryl-benzofuranone compounds. The antioxidant activities of those 3-aryl-benzofuranone compounds in polypropylene (PP) have been studied using the oxidation induction time (OIT) of PP stabilized by those 3-aryl-benzofuranone compounds too. It has been found t...