全文获取类型
收费全文 | 29822篇 |
免费 | 110篇 |
国内免费 | 115篇 |
专业分类
系统科学 | 171篇 |
丛书文集 | 451篇 |
教育与普及 | 36篇 |
理论与方法论 | 142篇 |
现状及发展 | 13434篇 |
研究方法 | 1416篇 |
综合类 | 13870篇 |
自然研究 | 527篇 |
出版年
2013年 | 249篇 |
2012年 | 506篇 |
2011年 | 1016篇 |
2010年 | 222篇 |
2008年 | 571篇 |
2007年 | 670篇 |
2006年 | 619篇 |
2005年 | 648篇 |
2004年 | 701篇 |
2003年 | 566篇 |
2002年 | 601篇 |
2001年 | 887篇 |
2000年 | 837篇 |
1999年 | 606篇 |
1992年 | 574篇 |
1991年 | 402篇 |
1990年 | 435篇 |
1989年 | 412篇 |
1988年 | 410篇 |
1987年 | 432篇 |
1986年 | 468篇 |
1985年 | 596篇 |
1984年 | 423篇 |
1983年 | 339篇 |
1982年 | 315篇 |
1981年 | 334篇 |
1980年 | 395篇 |
1979年 | 933篇 |
1978年 | 736篇 |
1977年 | 720篇 |
1976年 | 540篇 |
1975年 | 538篇 |
1974年 | 841篇 |
1973年 | 693篇 |
1972年 | 784篇 |
1971年 | 816篇 |
1970年 | 1133篇 |
1969年 | 900篇 |
1968年 | 772篇 |
1967年 | 817篇 |
1966年 | 758篇 |
1965年 | 568篇 |
1964年 | 190篇 |
1959年 | 277篇 |
1958年 | 533篇 |
1957年 | 368篇 |
1956年 | 279篇 |
1955年 | 260篇 |
1954年 | 301篇 |
1948年 | 190篇 |
排序方式: 共有10000条查询结果,搜索用时 968 毫秒
991.
Bazooka provides an apical cue for Inscuteable localization in Drosophila neuroblasts 总被引:1,自引:0,他引:1
Asymmetric cell division generates daughter cells with different developmental fates from progenitor cells that contain localized determinants. During this division, the asymmetric localization of cell-fate determinants and the orientation of the mitotic spindle must be precisely coordinated. In Drosophila neuroblasts, inscuteable controls both spindle orientation and the asymmetric localization of the cell-fate determinants Prospero and Numb. Inscuteable itself is localized in an apical cortical crescent and thus reflects the intrinsic asymmetry of the neuroblast. Here we show that localization of Inscuteable depends on Bazooka, a protein containing three PDZ domains with overall sequence similarity to Par-3 of Caenorhabditis elegans. Bazooka and Inscuteable form a complex that also contains Staufen, a protein responsible for the asymmetric localization of prospero messenger RNA. We propose that, after delamination of the neuroblast from the neuroepithelium, Bazooka provides an asymmetric cue in the apical cytocortex that is required to anchor Inscuteable. As Bazooka is also responsible for the maintenance of apical-basal polarity in epithelial tissues, it may be the missing link between epithelial polarity and neuroblast polarity. 相似文献
992.
Distribution of spatial and nonspatial information in dorsal hippocampus 总被引:10,自引:0,他引:10
The hippocampus in the mammalian brain is required for the encoding of current and the retention of past experience. Previous studies have shown that the hippocampus contains neurons that encode information required to perform spatial and nonspatial short-term memory tasks. A more detailed understanding of the functional anatomy of the hippocampus would provide important insight into how such encoding occurs. Here we show that hippocampal neurons in the rat are distributed anatomically in distinct segments along the length of the hippocampus. Each longitudinal segment contains clusters of neurons that become active when the animal performs a task with spatial attributes. Within these same segments are ordered arrangements of neurons that encode the nonspatial aspects of the task appropriate to those spatial features. Thus, anatomical segregation of spatial information, together with the interleaved representation of nonspatial information, represents a structural framework that may help to resolve conflicting views of hippocampal function. 相似文献
993.
It is usually assumed that, after construction of basic network architecture in embryos, immature networks undergo progressive maturation to acquire their adult properties. We examine this assumption in the context of the lobster stomatogastric nervous system. In the lobster, the neuronal population that will form this system is at first orgnanized into a single embryonic network that generates a single rhythmic pattern. The system then splits into different functional adult networks controlled by central descending systems; these adult networks produce multiple motor programmes, distinctively different from the single output of the embryonic network. We show here that the single embryonic network can produce multiple adult-like programmes. This occurs after the embryonic network is silenced by removal of central inputs, then pharmacologically stimulated to restore rhythmicity. Furthermore, restoration of the flow of descending information reversed the adult-like pattern to an embryonic pattern. This indicates that the embryonic network possesses the ability to express adult-like network characteristics, but descending information prevents it from doing so. Functional adult networks may therefore not necessarily be derived from progressive ontogenetic changes in networks themselves, but may result from maturation of descending systems that unmask preexisting adult networks in an embryonic system. 相似文献
994.
Identification of in vivo substrates of the chaperonin GroEL 总被引:22,自引:0,他引:22
995.
A plant regulator controlling development of symbiotic root nodules 总被引:37,自引:0,他引:37
996.
997.
998.
999.
Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana 总被引:21,自引:0,他引:21
Lin X Kaul S Rounsley S Shea TP Benito MI Town CD Fujii CY Mason T Bowman CL Barnstead M Feldblyum TV Buell CR Ketchum KA Lee J Ronning CM Koo HL Moffat KS Cronin LA Shen M Pai G Van Aken S Umayam L Tallon LJ Gill JE Adams MD Carrera AJ Creasy TH Goodman HM Somerville CR Copenhaver GP Preuss D Nierman WC White O Eisen JA Salzberg SL Fraser CM Venter JC 《Nature》1999,402(6763):761-768
Arabidopsis thaliana (Arabidopsis) is unique among plant model organisms in having a small genome (130-140 Mb), excellent physical and genetic maps, and little repetitive DNA. Here we report the sequence of chromosome 2 from the Columbia ecotype in two gap-free assemblies (contigs) of 3.6 and 16 megabases (Mb). The latter represents the longest published stretch of uninterrupted DNA sequence assembled from any organism to date. Chromosome 2 represents 15% of the genome and encodes 4,037 genes, 49% of which have no predicted function. Roughly 250 tandem gene duplications were found in addition to large-scale duplications of about 0.5 and 4.5 Mb between chromosomes 2 and 1 and between chromosomes 2 and 4, respectively. Sequencing of nearly 2 Mb within the genetically defined centromere revealed a low density of recognizable genes, and a high density and diverse range of vestigial and presumably inactive mobile elements. More unexpected is what appears to be a recent insertion of a continuous stretch of 75% of the mitochondrial genome into chromosome 2. 相似文献
1000.
A structural change in the kinesin motor protein that drives motility 总被引:34,自引:0,他引:34
Rice S Lin AW Safer D Hart CL Naber N Carragher BO Cain SM Pechatnikova E Wilson-Kubalek EM Whittaker M Pate E Cooke R Taylor EW Milligan RA Vale RD 《Nature》1999,402(6763):778-784
Kinesin motors power many motile processes by converting ATP energy into unidirectional motion along microtubules. The force-generating and enzymatic properties of conventional kinesin have been extensively studied; however, the structural basis of movement is unknown. Here we have detected and visualized a large conformational change of an approximately 15-amino-acid region (the neck linker) in kinesin using electron paramagnetic resonance, fluorescence resonance energy transfer, pre-steady state kinetics and cryo-electron microscopy. This region becomes immobilized and extended towards the microtubule 'plus' end when kinesin binds microtubules and ATP, and reverts to a more mobile conformation when gamma-phosphate is released after nucleotide hydrolysis. This conformational change explains both the direction of kinesin motion and processive movement by the kinesin dimer. 相似文献