Oxidation of cyclophosphamide by means of KMnO4

Zusammenfassung Nach Oxidation von Cyclophosphamid mit KMnO₄ lässt sich eine Verbindung gewinnen, die dünnschichtchromatographisch und reaktionskinetisch mit dem vorherrschenden Urinmetaboliten übereinstimmt. Es handelt sich um N,N-bis(2-chloräthyl) diamidophosphorsäure (2-carboxyäthylester). Da weder diese Verbindung noch das bereits als Nebenmetabolit bekannte 4-Keto-cyclophosphamids zelltoxisch wirkt, muss der Effekt des Cyclophosphamids von einem anderen Stoffwechselprodukt ausgehen. Durch modifizierte oxidative Umsetzung in vitro sollte es möglich sein, Cyclophosphamid auch in den wirksamen Metaboliten zu überführen.     

Fungistatic action of the pigment secreted by the fungusEpicoccum nigrum link

Résumé On a constaté que le pigment sécrété par le mycèteEpicoccum nigrum a une réaction fungistatique sur leBoyrytis allii utilisé comme un organisme d'essai. Cette réaction a été dépendante du pH et a été assez basse aux niveaux dépassant 5.0.     

Microtubules with more than 13 protofilaments in the dividing nuclei of ciliates

Microtubules are largely composed of proteins called tubulins. These are stacked in linear arrays called protofilaments (p). Most microtubules have precisely 13p (ref. 1). The 'incomplete' B and C microtubules (10 or 11p) of cilia, flagella, basal bodies and centrioles are widespread exceptions. Very few examples of 'complete' microtubules with more, or less, than 13p have been found. However, the 'ciliate cell' includes a larger number of highly differentiated types of microtubule arrays than most other cell types. The present study was undertaken to ascertain whether there is variation in p number in two ciliates. In both, all complete cytoplasmic microtubules examined have 13p but microtubules with 13-16p are present in the nucleoplasm of dividing nuclei. These features are probably common to ciliates in general because the free-living hymenostone Paramecium tetraurelia and the parasitic heterotrich Nycotherus ovalis are not closely related in terms of taxonomic criteria or life-style.     

Bestimmung des α-2-Makroglobulinumsatzes beim Menschen

Summary The turnover of ₂-macroglobulins was measured by quantitative precipitation of the I¹³¹-marked protein, using a specific antiserum. In 6 normal persons, the half-life was found to be between 192 and 267 h. The catabolic rates in relation to the intravascular pool range between 437 and 703 mg/day

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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft.     

Zur Frage der genetischen Änderung eines Ascites-Hepatoms der Ratte durch Heterotransplantation auf chinesische Hamster

Summary Heterotransplantation of the rat hepatoma of Zajdela into golden hamsters, mice and Chinese hamsters has induced virtually no change in the neoplasm. The host tissues have shown, however, a 1% increase in their own mitoses, which is attributed to growth stimulation by the tumor.