SPME-GC-MS法比较野生和栽培铁皮石斛的挥发性成分

采用固相微萃取(SPME)技术,气质联用(GC-MS)法分析野生和栽培铁皮石斛的挥发性成分.从野生和栽培铁皮石斛中,分别鉴定出97种和80种挥发性成分,相对含量均占总离子峰的96.0%,具有60个相同的化合物,主要成分均包含芳樟醇、癸醛、对映-海松-8(14),15-二烯等化合物.     

Crystal structure of the transfer-RNA domain of transfer-messenger RNA in complex with SmpB

Accurate translation of genetic information into protein sequence depends on complete messenger RNA molecules. Truncated mRNAs cause synthesis of defective proteins, and arrest ribosomes at the end of their incomplete message. In bacteria, a hybrid RNA molecule that combines the functions of both transfer and messenger RNAs (called tmRNA) rescues stalled ribosomes, and targets aberrant, partially synthesized, proteins for proteolytic degradation. Here we report the 3.2-A-resolution structure of the tRNA-like domain of tmRNA (tmRNA(Delta)) in complex with small protein B (SmpB), a protein essential for biological functions of tmRNA. We find that the flexible RNA molecule adopts an open L-shaped conformation and SmpB binds to its elbow region, stabilizing the single-stranded D-loop in an extended conformation. The most striking feature of the structure of tmRNA(Delta) is a 90 degrees rotation of the TPsiC-arm around the helical axis. Owing to this unusual conformation, the SmpB-tmRNA(Delta) complex positioned into the A-site of the ribosome orients SmpB towards the small ribosomal subunit, and directs tmRNA towards the elongation-factor binding region of the ribosome. On the basis of this structure, we propose a model for the binding of tmRNA on the ribosome.     

Trigger factor in complex with the ribosome forms a molecular cradle for nascent proteins

During protein biosynthesis, nascent polypeptide chains that emerge from the ribosomal exit tunnel encounter ribosome-associated chaperones, which assist their folding to the native state. Here we present a 2.7 A crystal structure of Escherichia coli trigger factor, the best-characterized chaperone of this type, together with the structure of its ribosome-binding domain in complex with the Haloarcula marismortui large ribosomal subunit. Trigger factor adopts a unique conformation resembling a crouching dragon with separated domains forming the amino-terminal ribosome-binding 'tail', the peptidyl-prolyl isomerase 'head', the carboxy-terminal 'arms' and connecting regions building up the 'back'. From its attachment point on the ribosome, trigger factor projects the extended domains over the exit of the ribosomal tunnel, creating a protected folding space where nascent polypeptides may be shielded from proteases and aggregation. This study sheds new light on our understanding of co-translational protein folding, and suggests an unexpected mechanism of action for ribosome-associated chaperones.     

汉语中关于“死”的委婉语及其会话原则

会话的合作原则和礼貌原则是语用学研究的一个重要内容，人们在交际中要遵循合作原则，但在具体语境中却可能由于礼貌而故意违反合作原则，以求达到更好的交际效果。汉语中关于“死”的委婉语主要是体现礼貌原则当中的两个准则，礼貌原则受到具体社会文化的影响，人们在交际过程中在考虑礼貌原则的同时也是在寻求其文化适应性的过程。     

Evaluation of the transfer function of human pulse system based on signal detection

On the basis of the pulse source-system model, a convenient and effective method used to evaluate the transfer function of human pulse system has been proposed by using the principle of signal detection and system analysis. The experimental results show that the pulse system of the normal pulse has 3 formants; the smooth pulse, 2 formants; the wiry pulse, 4 formants; and the thready pulse, only 1 formant. Formant frequencies reflect the resonance behaviour of the arterial system.     

由高斯核确定的卷积函数类上的n-宽度

研究了由高斯核确定的多元卷积函数类上的n-宽度,并得到了某些情况下宽度的渐近阶.     

强磁场中NO分子的模型势与闭合轨道

我们采用一种全新的方法来计算强外磁场中高里德堡态NO分子的模型势,此势把多通道的几何对称性考虑进去,以致此势在长程区和短程区都是适用的．利用哈密顿量的经典正则方程,我们还得到各通道的经典闭合轨道,从而为NO分子回归谱的计算做好准备．     

基于抗白噪声理论的支持向量机

支持向量机（SVM）是在统计学习理论基础上发展起来的一种新的机器学习方法.具有泛化能力强,全局最优等特点.我们针对于传统的支持向量机算法忽略了当采取的训练集中有噪声干扰的情况,通过改造原有的经验风险和调节核函数中的参数,达到抑制或者减弱随机噪声干扰的目的,并具体地给出了抗高斯白噪声的支持向量机模型.     

初中物理实验目标评估初探

本项实验主要对初中物理学生实验这一薄弱环节进行了研究。文章介绍了＂实验的方法与过程＂，＂初中物理学生实验能力目标＂、＂初中物理学生实验达标评估细则＂．．．．．．。限于篇幅，删去了具体的事例及实验结论篇。     

在HBV和(或)AFB1诱发树鼩肝癌过程中肝组织ras癌基因的表达

为了解在人乙型肝炎病毒（ＨＢＶ）和／或黄曲霉毒素（ＡＦＢ１）诱发树Ｑｕ肝癌过程中Ｐ＾２１在肝组织的表达情况,用免疫组化方法观察人ＨＢＶ＋ＡＦＢ１双因素组（Ａ组）、单因素ＨＢＶ组（Ｂ组）、单因素ＡＦＢ１组（Ｃ组）、及对照组（Ｄ组）实验第４４周、１０５周、１１９周动物肝活检组织Ｐ＾２１蛋白的表达情况。结果,至１１９周,累计肝组织Ｐ＾２１阳性动物百分率分别为３５．３、５．３、１３．３、０。表明人ＨＢＶ与