Genistein inhibits human TNF-α-induced porcine endothelial cell adhesiveness for human monocytes and natural killer cells

Cellular immune response is a major barrier to xenotransplantation. Human tumor necrosis factor-α (hTNF-α) possesses cross-species activity and directly amplifies the immune rejection via the upregulation of adhesion molecules on porcine endothelium. We investigated the role of protein tyrosine phosphorylation in the induction of expression of E-sclectin and vascular cell adhesion molecule-1 (VCAM-1), and the augmentation of adhesion of human peripheral blood monocytes (PBMo) and natural killer cells (PBNK), after rhTNF-α-stimulation of porcine aortic endothelial cells (PAEC) in vitro, rhTNF-α-increased adhesiveness of PAEC for both PBMo and PBNK was dose-dependently reduced by pretreatment of PAEC with the selective protein tyrosine kinase (PTK) inhibitor genistein. The inhibitory effect occurred at the early time of PAEC activation triggered by rhTNF-α, and was completely reversible. PTK activity assay indicated that genistein also suppressed rhTNF-α stimulated activation of protein tyrosine kinases (PTKs) in PAEC in a dose-dependent manner. Flow cytometric analysis showed that genistein inhibited the upregulation of E-selectin and VCAM-1 by rhTNF-α. These results suggest that PTKs may regulate the expression of E-selectin and VCAM-1 on PAEC and the adherence of PBMo and PBNK induced by rhTNF-α. Moreover, dietary genistein, used as an adhesion antagonist, may contribute to managing the cell-mediated rejection in the clinical application.     

眼镜蛇神经毒素低分子量水解物的镇痛研究

眼镜蛇神经毒素经胃液水解后超滤得分子量〈３０００ｕ低分子量ｃｏｂｒｏｔｏｘｉｎ胃液水解物。该低分子量产物在小鼠热板及大鼠电尾嘶叫测痛实验，都显示出明显的镇痛作用，并有剂量一效应关系。     

A4分子的定性分子轨道处理

本文用定性分子轨道理论和碎片轨道法构造了氢原子簇Ｈ４和代表性元素磷的四原子簇合物Ｐ４的分子轨道。可得分子轨道的形状和相对能级次序与定理计算结果一致。     

Effects of kallidinogenase on urinary kallikrein excretion and plasma prostanoid concentrations in patients with essential hypertension

The effects of kallidinogenase on urinary kallikrein excretion, plasma immunoreactive prostanoids and platelet aggregation were investigated in patients with essential hypertension. Urinary kallikrein excretion and plasma 6-keto PGF1 alpha concentration were significantly decreased in these patients. Significant decreases in blood pressure, as well as significant increases of urinary kallikrein excretion and plasma 6-keto PGF1 alpha concentration after kallidinogenase administration were also observed.     

交换桥臂测量电阻消除系统误差的条件

本文通过理论推导和实验方法,阐述用交换桥臂测量电阻消除系统误差必须具备两个条件．     

《小企业会计制度》与《企业会计制度》的比较

在社会主义市场经济日益活跃的今天,小企业的地位和作用日益突出,但同时也存在一些问题.本文通过<小企业会计制度>与<企业会计制度>的比较,旨在明晰小企业会计在实际工作中的内容,引导其向规范方向发展.     

绿色糖单孢菌麦芽糖α-淀粉酶基因在枯草芽孢杆菌中的高效分泌表达

【目的】构建高产麦芽糖α-淀粉酶的工程菌株并实现高效分泌表达。【方法】PCR扩增麦芽糖α-淀粉酶基因sva,再与大肠杆菌-枯草芽孢杆菌麦芽糖诱导型穿梭载体pHCMCO4-Pglv连接,构建重组质粒pHCMCO4-Pglv-sva并转入枯草芽孢杆菌进行表达,对重组酶进行SDS-PAGE分析,然后对重组菌株的生长及发酵条件进行优化。【结果】成功构建重组质粒pHCMCO4-Pglv-sva并在枯草芽孢杆菌中实现分泌表达,SDS-PAGE分析发现,在55kDa处得到特异性蛋白条带。单因素试验结果显示,重组菌的最适诱导温度为35℃,最适接种量为4%(V/V),最适装液量为30mL。正交实验结果显示,重组菌的最佳发酵条件组合是诱导温度37℃、接种量5%(V/V)、装液量25mL,在此条件下发酵液粗酶活力达到257.3698U/mL。装液量对重组菌的产酶量影响显著。【结论】成功构建能高效分泌表达麦芽糖α-淀粉酶的枯草芽孢杆菌工程菌株,经发酵条件优化后,重组酶产量显著提高10倍左右。     

高职院校《IT职业英语》课程教学设计

对于高职院校软件技术专业即将就业的学生来说,具备IT职业英语能力是至关重要的。《IT职业英语》是一门融英语语言技能和IT职业技能为一体的综合职业技能课程。教学内容组织遵循阶段性和循序渐进原则,辅助相应的教学模式和方法,以实现该课程的目标。     

土壤中重金属污染源分析研究

针对某市表层土壤分析数据,判断该市各功能区污染程度和污染源.由单因子指数分析和富集系数分布图可知,金属Cu、Hg、Cd、Pb在该市工业区属于重度污染,As、Cu、Cd、Hg和Pb在该市生活区为重度污染,在主干道路区Hg、Cd和Pb呈中、重度污染.由卡梅罗综合污染指数得出该市功能区污染程度依次为工业区、主干道路区、生活区、公园绿地区和山区.金属空间分布图表示元素的浓度较高点均集中在工业区、主干道路区和生活区,而由主成分分析能定量的描述出Hg、Cu、Pb、As、Cd在主成分中占较大比重,由R型聚类分析知Pb、As、Hg具有较大的相关性,由此可知由此城市土壤污染主要来源于工业污水排放、生活垃圾以及汽车尾气的排放.     

离子膜蒸发工序蒸汽冷凝水回收再利用

烧碱浓缩的过程中会产生大量的冷凝水,主要作为凉水塔的补充水和机泵冷却水。由于冷凝水温度较高,其回收再利用前需要进行冷却,造成热量的浪费。介绍了离子膜蒸发系统含碱冷凝水循环再利用的改造措施,提出将不同温度的冷凝水分开,将温度高的部分送至化盐工序,不仅能将热量充分利用起来,还可以节约大量生产用水。