全文获取类型
收费全文 | 2254篇 |
免费 | 63篇 |
国内免费 | 118篇 |
专业分类
系统科学 | 94篇 |
丛书文集 | 86篇 |
教育与普及 | 166篇 |
理论与方法论 | 11篇 |
现状及发展 | 15篇 |
研究方法 | 7篇 |
综合类 | 2052篇 |
自然研究 | 4篇 |
出版年
2024年 | 15篇 |
2023年 | 35篇 |
2022年 | 52篇 |
2021年 | 55篇 |
2020年 | 30篇 |
2019年 | 3篇 |
2018年 | 19篇 |
2017年 | 31篇 |
2016年 | 19篇 |
2015年 | 46篇 |
2014年 | 60篇 |
2013年 | 59篇 |
2012年 | 74篇 |
2011年 | 94篇 |
2010年 | 93篇 |
2009年 | 127篇 |
2008年 | 132篇 |
2007年 | 125篇 |
2006年 | 73篇 |
2005年 | 76篇 |
2004年 | 48篇 |
2003年 | 60篇 |
2002年 | 64篇 |
2001年 | 77篇 |
2000年 | 77篇 |
1999年 | 122篇 |
1998年 | 87篇 |
1997年 | 109篇 |
1996年 | 99篇 |
1995年 | 98篇 |
1994年 | 97篇 |
1993年 | 70篇 |
1992年 | 39篇 |
1991年 | 37篇 |
1990年 | 53篇 |
1989年 | 31篇 |
1988年 | 29篇 |
1987年 | 13篇 |
1986年 | 5篇 |
1985年 | 2篇 |
排序方式: 共有2435条查询结果,搜索用时 19 毫秒
161.
Intrusion detection using rough set classification 总被引:6,自引:0,他引:6
Recently machine learning-based intrusion detection approaches have been subjected to extensive researches because they can detect both misuse and anomaly. In this paper, rough set classification (RSC), a modern learning algorithm, is used to rank the features extracted for detecting intrusions and generate intrusion detection models. Feature ranking is a very critical step when building the model. RSC performs feature ranking before generating rules, and converts the feature ranking to minimal hitting set problem addressed by using genetic algorithm (GA). This is done in classical approaches using Support Vector Machine (SVM) by executing many iterations, each of which removes one useless feature. Compared with those methods, our method can avoid many iterations. In addition, a hybrid genetic algorithm is proposed to increase the convergence speed and decrease the training time of RSC. The models generated by RSC take the form of "IF-THEN" rules, which have the advantage of explication. Tests and comparison of RSC with SVM on DARPA benchmark data showed that for Probe and DoS attacks both RSC and SVM yielded highly accurate results (greater than 99% accuracy on testing set). 相似文献
162.
163.
BAIYong GONGWei LIUTianyun ZHUYuxian 《科学通报(英文版)》2003,48(20):2221-2225
Cinnamoyl CoA reductase (CCR: EC 1.2.1.44),the entry-point enzyme of the llgnin specific biosynthetic pathway, catalyzes the conversion of cinnamoyl CoA esters to their corresponding dnnamaldehydes. Multiple sequence alignment showed that the deduced polypeptide shared 70% similarity and 30% sequence identity at the amino acid level with defined CCR genes from other plant species and they all contain the common signature sequences thought to be the catalytic site as well as the putative NADP binding domain.Using a conserved OsCCR cDNA fragment as the probe for library screening, we isolated the genomic DNA that covered the whole coding region of OsCCR with total length of 3045bp including 4 introns and 5 exons. The open reading frame for our OsCCR gene coBtAin~ 337 amino adds. Northern blot indicated that OsCCR was expressed in different organs with the highest level found in stems. In situ hybridization results showed that OsCCR mRNA was localized mainly along the vascular bundles in stems and leaves, and also in lateral roots that was differentiating from the tiilering node. We conclude that the vascular-localized expression of OsCCR gene may suggest its possible involvement in llgnin biosynthesis. Cloning and characterization of OsCCR will help to clarify how llgniflcations in plants are regulated and will provide a physical basis for creating genetically engineered rice plants with optimal lignin contents. 相似文献
166.
运用ANSYS软件对空心板桥水泥混凝土铺装层进行实体建模,并针对铺装层破坏形式开展力学分析,提出铺装层破坏的解决办法. 相似文献
167.
168.
170.
Modification of the rib operon derived from Bacillus subtilis and its expression in Escherichia coli
张会图 Meng Kun Wang Yaru Luo Huiying Yuan Tiezheng Yang Peilong Bai Yingguo Yao Bin Fan Yunliu 《高技术通讯(英文版)》2007,13(1):85-90
A riboflavin operon (rib operon) derived from Bacillus subtilis 368 was modified on structure and the resulting operons were expressed in various strains of Escherichia coli. The results showed that the optimization of the rib operon and the host strain used for expression are two main factors affecting the riboflavin production. Replacing the promoterl and rfn box of the rib operon with a strong constructive promoter spol drastically increased the expression of the rib genes. When E. coli JM109 was used as the host strain, the highest riboflavin production reached 95.3μg/mL (about eight times higher than that of the unmodified r/b operon). In addition, when tetracycline (20 μg/mL) was used as the selective pressure, compared with the ampicillin resistant transformants, a higher riboflavin yield was obtained in tetracycline resistant host strain. 相似文献