人免疫缺陷病毒2型(HIV-2)穿膜蛋白基因片段克隆表达

以人免疫缺陷病毒２（ Ｈ Ｉ Ｖ２） 的原病毒基因组为模板,通过套式 Ｐ Ｃ Ｒ 扩增得到了 Ｈ Ｉ Ｖ２ 的一段特异性膜抗原蛋白基因片段;随后将此片段克隆入诱导表达载体ｐ Ｇ Ｅ Ｘ５ Ｘ１ ,获得了重组表达质粒ｐ Ｇ Ｅ Ｘ３６ Ｅ Ｎ Ｖ． Ｉ Ｐ Ｔ Ｇ 对重组表达菌株诱导后, Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 结果显示重组菌株有特异性表达蛋白带产生     

用PB实现Foxpro与Oracle之间的数据转移

讲述了在ＰｏｗｅｒＢｉｕｌｄ６．０中实现一种简便的数据转移的方法。     

基于动态温度调控的空调系统能耗预测

针对动态温度调控的空调系统能耗预测问题,设计了动态温度调控策略并通过EnergyPlus仿真得到空调系统逐时能耗数据集.在采用集成方法分析能耗的基础上,建立改进PSO算法优化BP神经网络(improved particle swarm optimization-back propagation neural netwo...     

深井泵柱塞修复工艺的探讨

机械设备中金属件的修复技术主要有:堆焊、热喷涂、槽镀和电刷镀。文中对四种金属件修复技术进行分析对比,并结合稠油深井泵柱塞的失效特点,说明了电刷镀是稠油深井泵柱塞修复的最隹方法,给出了电刷镀工艺流程及基本参数。     

大针茅(Stipa grandis)和克氏针茅(S.krylovii)对PEG渗透胁迫适应性反应的比较研究

本文以大针茅（Stipa grandis）和克氏针茅（S．krylovii）为实验对象,研究PEG渗透胁迫下,两种植物的种子发芽情况以及不同龄期植株叶片含水量、叶片延伸速率、游离脯氨酸含量与根冠比的变化特征．结果表明：（1）PEG胁迫对大针茅萌发的影响大于克氏针茅;（2）相同龄期的克氏针茅比大针茅更能适应渗透胁迫;（3）四月龄植株较二月龄植株具有更高的胁迫适应性．以上结果为认识大针茅和克氏针茅的干旱适应性规律提供了部分实验证据．     

基于APDL语言的结构优化设计

以三杆桁架为例,介绍了运用APDL（Ansys Parameter Design Language）语言进行参数化建模及结构优化设计的过程,得出了参数的优化结果.该优化过程表明,运用APDL进行优化设计的方法避免了大量的重复有限元建模和前后处理操作,明显提高了产品的设计效率.     

西峡县香菇生产管理中存在的问题与对策

西峡县是我国食用菌生产大县,也是代料香菇生产大县。该产业的健康发展,直接关系着地方的财政增收及农民致富。近年来,西峡县食用菌产业不断发展壮大,但群众的科学种菇意识有所淡薄,直接影响了种菇的产量和效益,现进行分析。     

Association analyses identify multiple new lung cancer susceptibility loci and their interactions with smoking in the Chinese population

To find additional susceptibility loci for lung cancer, we tested promising associations from our previous genome-wide association study (GWAS) of lung cancer in the Chinese population in an extended validation sample size of 7,436 individuals with lung cancer (cases) and 7,483 controls. We found genome-wide significant (P < 5.0 × 10(-8)) evidence for three additional lung cancer susceptibility loci at 10p14 (rs1663689, close to GATA3, P = 2.84 × 10(-10)), 5q32 (rs2895680 in PPP2R2B-STK32A-DPYSL3, P = 6.60 × 10(-9)) and 20q13.2 (rs4809957 in CYP24A1, P = 1.20 × 10(-8)). We also found consistent associations for rs247008 at 5q31.1 (IL3-CSF2-P4HA2, P = 7.68 × 10(-8)) and rs9439519 at 1p36.32 (AJAP1-NPHP4, P = 3.65 × 10(-6)). Four of these loci showed evidence for interactions with smoking dose (P = 1.72 × 10(-10), P = 5.07 × 10(-3), P = 6.77 × 10(-3) and P = 4.49 × 10(-2) for rs2895680, rs4809957, rs247008 and rs9439519, respectively). These results advance our understanding of lung cancer susceptibility and highlight potential pathways that integrate genetic variants and smoking in the development of lung cancer.     

Genome-wide association identifies a susceptibility locus for coronary artery disease in the Chinese Han population

Coronary artery disease (CAD) causes more than 700,000 deaths each year in China. Previous genome-wide association studies (GWAS) in populations of European ancestry identified several genetic loci for CAD, but no such study has yet been reported in the Chinese population. Here we report a three-stage GWAS in the Chinese Han population. We identified a new association between rs6903956 in a putative gene denoted as C6orf105 on chromosome 6p24.1 and CAD (P = 5.00 × 10?3, stage 2 validation; P = 3.00 × 10?3, P = 1.19 × 10?? and P = 4.00 × 10?3 in three independent stage 3 replication populations; P = 4.87 × 10?12, odds ratio = 1.51 in the combined population). The minor risk allele A of rs6903956 is associated with decreased C6orf105 mRNA expression. We report the first GWAS for CAD in the Chinese Han population and identify a SNP, rs6903956, in C6orf105 associated with susceptibility to CAD in this population.     

静电放电电磁脉冲场对针板间隙流注放电的影响分析

为了分析自然环境下静电放电电磁脉冲场对针板间隙流注放电的影响,基于流体动力学理论,建立静电放电电磁脉冲场下流注放电模型,对大气压下10mm针板间隙流注放电过程进行了仿真,研究了静电电磁脉冲场对流注运动发展过程中电场与光子通量的影响,以及得到不同静电电磁脉冲场下流注放电间隙贯穿时间的变化规律。结果表明:无静电电磁脉冲场时,随着时间的推移流注不断向阴极发展,当流注头部靠近阴极时,流注头部电场强度逐渐增大,光子通量幅值亦迅速增长;通入静电电磁脉冲场后,相同时间内,电场向板极推进的速度明显加快,击穿电压阈值显著降低,随着静电放电输出电压的增加,流注贯穿间隙的时间逐渐减小。对于强电磁场环境下电子设备安全防护的研究具有参考意义。