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71.
The effects of initial sucrose concentration, nitrate to ammonium ratio, total N concentration and phosphate concentration in medium on cell growth and isocamptothecin A and B synthesis by suspension call culture of Camptotheca acuminata were investigated in 250 mL shake flasks. 30 g L^-1 sucrose concentration was beneficial to secondary metabolites synthesis. The cell growth and metabolites synthesis were also affected by the ratio of NO3^-/NH4^+ , and nitrate was tavourable for cell growth. The maximum dry weight was achieved when nitrate was used as the sole N souree. The effect of total initial N on the cell cultures was also investigated with NO3^-/NH4^+ ratio of 1 : 2. The final dry cell weight was similar throughout culture period and 50 mM initial N was favourable for secondary metabolite synthesis. 50 mM initial phosphate concentration facilitated both cell growth and secondary metabolites synthesis. 相似文献
72.
73.
采用聚季铵盐修饰的方法合成了水溶性的多壁碳纳米管(MWNTs)。用混酸(浓硫酸与浓硝酸,体积比为3∶1)对平均直径为20 nm的MWNTs进行氧化处理。红外光谱分析表明,氧化1 h~3 h可使MWNTs表面生成羧基,扫描电镜测试表明MWNTs被氧化为短管。氧化2 h的MWNTs经过与氯化亚枫的酰氯化及与聚季铵盐的脂化缩合两个反应步骤,合成了表面接枝聚季铵盐的MWNTs,红外光谱分析证明了该MWNTs的生成。透射电镜测试显示聚季铵盐修饰的MWNTs在乙醇中能够有效地克服团聚倾向;水溶性实验表明改性后的MWNTs在水中具有自分散性,并能够保持长时间的溶解能力。 相似文献
74.
The West Palearctic species of Rhoptromeris are revised. A total of 11 species are recognised as valid in this region, including four newly described species: Rhoptromeris dichromata sp. nov., Rhoptromeris koponeni sp. nov., Rhoptromeris leptocornis sp. nov. and Rhoptromeris macaronesiensis sp. nov. Eucoila luteicornis Ionescu, 1959 is synonymised with Rhoptromeris heptoma (Hartig, 1840) syn. nov. A checklist of the Holarctic Rhoptromeris is presented and an identification key to the West Palearctic species is provided. www.zoobank.org/urn:lsid:zoobank.org:pub:8164332C-93E2-4E3F-A408-F5FF5DFB366E 相似文献
75.
为探索介质流向对截止阀内部流场和流阻特性的影响,应用SOLIDWORKS软件建立截止阀的三维模型。使用Fluent软件中的有限元法和标准k-ε湍流模型,在不同开度的情况下,采用数值模拟分析的方法,研究不同介质流向下截止阀的流场和流阻特性,分析阀体内部的速度和压力分布规律。结果表明:在两种不同的流向下,阀门的流通能力相差较小;在小开度时,介质高进低出时能够减小阀门的压力损失,在流通高压介质的情况下,高进低出的流向能够极大的减小阀门的关闭力。流场的分析为截止阀的结构设计和优化提供参考。 相似文献
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Mid-troposphere CO2 data retrieved by the AIRS (atmospheric infrared sounder) were validated with five ground-based stations and aircraft measurements in the Northern Hemisphere. AIRS CO2 products show good agreement with ground and aircraft observations. The data had a monthly average accuracy better than 3 ppmv. In this study, the spatial and temporal distribution of mid-troposphere CO2 from January 2003 to December 2008 was analyzed based on this satellite product. The average concentration of atmospheric CO2 was higher in the Northern Hemisphere than in the Southern Hemisphere. The yearly average results show a gradual increase from 2003 to 2008. In China, the annual growth rate was about 2 ppmv/a, similar to the United States, Europe, Australia and India, but was slightly lower than Canada and Russia. Mid-troposphere CO2 concentrations were higher over northern China than over southern areas, due to differences in natural conditions and industrial layout. There were four centers of high CO2 concentration between 35° and 45°N over China, with low concentrations over Yunnan Province. There was a significant seasonal CO2 variation with peak concentration in spring and the lowest concentration in autumn. 相似文献
78.
将巴斯德毕赤酵母异源表达出的疏水蛋白rHGFI和rHFBI修饰硅化玻璃后进行玻璃表面接触角测定.结果显示:蛋白处理的硅化玻璃表面由疏水变为亲水.蛋白溶液表面张力测定结果表明:rHGFI和rHFBI是表面活性非常强的蛋白,均可使表面张力降低.以常用的食品乳化剂酪蛋白酸钠为对照,检测rHGFI和rHFBI蛋白的发泡能力和泡沫稳定性.0.1%的rHGFI和rHFBI蛋白溶液发泡能力明显高于同浓度的酪蛋白酸钠溶液,rHFBI蛋白溶液的泡沫稳定性最佳. 相似文献
79.
80.
Leaf rust caused by Puccinia triticina is an economically-important disease in wheat worldwide.A combination of different types of resistance genes may significantly enhance rust resistance under rust-favorable conditions.To investigate the interactions between the rust resistance gene Lr34 and the lesion mimic gene lm on 1BL in Ning 7840,a segregating F8-10 population of 180 recombinant inbred lines was developed from Ning 7840/Chokwang and evaluated for both lesion mimic expression and leaf rust response at the adult plant stage in a greenhouse.A major quantitative trait locus(QTL),derived from Sumai 3,was co-localized with Lr34 on chromosome 7D and explained 41.5% of phenotypic variations for rust severity and 22.1% for leaf tip necrosis(LTN).The presence of Lr34 was confirmed by Lr34-specific markers cssfr1 and cssfr2 in Ning 7840 and Sumai 3.Unlike Lr34,lm conditioned a spontaneous lesion mimic phenotype and had a significant effect on reducing uredinial size,and a smaller effect on severity.Additive effects were observed between lm and Lr34 for severity and LTN,and an epistatic effect was observed for infection type.Single marker analysis also identified several other QTL with minor effects on severity,infection type,or LTN. 相似文献