排序方式: 共有53条查询结果,搜索用时 15 毫秒
41.
Pyrexia is a new thermal transient receptor potential channel endowing tolerance to high temperatures in Drosophila melanogaster 总被引:2,自引:0,他引:2
Lee Y Lee Y Lee J Bang S Hyun S Kang J Hong ST Bae E Kaang BK Kim J 《Nature genetics》2005,37(3):305-310
Several transient receptor potential channels were recently found to be activated by temperature stimuli in vitro. Their physiological and behavioral roles are largely unknown. From a temperature-preference behavior screen of 27,000 Drosophila melanogaster P-insertion mutants, we isolated a gene, named pyrexia (pyx), encoding a new transient receptor potential channel. Pyx was opened by temperatures above 40 degrees C in Xenopus laevis oocytes and HEK293T cells. It was ubiquitously expressed along the dendrites of a subset of peripheral nervous system neurons and was more permeable to K(+) than to Na(+). Although some pyx alleles resulted in abnormal temperature preferences, pyx null flies did not have significantly different temperature preferences than wild-type flies. But 60% of pyx null flies were paralyzed within 3 min of exposure to 40 degrees C, whereas only 9% of wild-type flies were paralyzed by the same stimulus. From these findings, we propose that the primary in vivo role of Pyx is to protect flies from high-temperature stress. 相似文献
42.
Regulation of ion transport by phosphorylation and G proteins occurs in several epithelial and non-epithelial cell plasma membranes1-5. It is not known whether transporters on intracellular membranes are target sites for second messengers. Here we present direct evidence that a chloride conductance in endocytic vesicles from rabbit proximal tubule is activated by phosphorylation through a cyclic AMP-dependent protein kinase. To measure chloride transport, endocytic vesicles were labelled in vivo with a Cl(-)-sensitive fluorescent indicator6-8. It was found that labelled endosomes contained an inward proton pump and a chloride conductance, but no ion-coupled chloride transport, and that the chloride conductance was regulated by protein kinase A. These results, taken together with measurements of chloride effects on ATP-dependent acidification, suggest that endosomal pH can be controlled by phosphorylation of a stilbene-sensitive conductive chloride transporter. 相似文献
43.
Decision trees are mainly used to classify data and predict data classes. A spatial decision tree has been designed using Euclidean distance between objects for reflecting spatial data characteristic. Even though this method explains the distance of objects in spatial dimension, it fails to represent distributions of spatial data and their relationships. But distributions of spatial data and relationships with their neighborhoods are very important in real world. This paper proposes decision tree based on spatial entropy that represents distributions of spatial data with dispersion and dissimilarity. The rate of dispersion by dissimilarity presents how related distribution of spatial data and non-spatial attributes. The experiment evaluates the accuracy and building time of decision tree as compared to previous methods and it shows that the proposed method makes efficient and scalable classification for spatial decision support. 相似文献
44.
A highway distress and preventive measures decision support system based on WebGIS and J2EE architecture were studied, and the key issues for the system are presented. Such issues as spatial data and attribute data organization, technology of enhancing user experience, database connection pool were investigated. The feasibility of the theories and methods were tested through the system. 相似文献
45.
This study is the second report on the genus Promalactis, which includes five new species, P. ceratiscus sp. nov., P. flavidius sp. nov., P. latifasciata sp. nov., P. mecodigita sp. nov. and P. petasumella sp. nov., from western and southern Cambodia. Also provided is a taxonomic key to all species of Cambodian Promalactis.
http://www.zoobank.org/urn:lsid:zoobank.org:pub:72474144-5D65-48BE-9021-0490952AAA3C 相似文献
46.
Raychaudhuri S Sandor C Stahl EA Freudenberg J Lee HS Jia X Alfredsson L Padyukov L Klareskog L Worthington J Siminovitch KA Bae SC Plenge RM Gregersen PK de Bakker PI 《Nature genetics》2012,44(3):291-296
The genetic association of the major histocompatibility complex (MHC) to rheumatoid arthritis risk has commonly been attributed to alleles in HLA-DRB1. However, debate persists about the identity of the causal variants in HLA-DRB1 and the presence of independent effects elsewhere in the MHC. Using existing genome-wide SNP data in 5,018 individuals with seropositive rheumatoid arthritis (cases) and 14,974 unaffected controls, we imputed and tested classical alleles and amino acid polymorphisms in HLA-A, HLA-B, HLA-C, HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQB1 and HLA-DRB1, as well as 3,117 SNPs across the MHC. Conditional and haplotype analyses identified that three amino acid positions (11, 71 and 74) in HLA-DRβ1 and single-amino-acid polymorphisms in HLA-B (at position 9) and HLA-DPβ1 (at position 9), which are all located in peptide-binding grooves, almost completely explain the MHC association to rheumatoid arthritis risk. This study shows how imputation of functional variation from large reference panels can help fine map association signals in the MHC. 相似文献
47.
Yaya Wang Qiao Cao Qin Cao Jianhua Gan Ning Sun Cai-Guang Yang Taeok Bae Min Wu Lefu Lan 《科学通报(英文版)》2021,(11):1101-1118
During infections,bacteria stimulate host cells to produce and release histamine,which is a key mediator of vital cellular processes in animals.However,the mech... 相似文献
48.
Adrianto I Wen F Templeton A Wiley G King JB Lessard CJ Bates JS Hu Y Kelly JA Kaufman KM Guthridge JM Alarcón-Riquelme ME;BIOLUPUS GENLES Networks Anaya JM Bae SC Bang SY Boackle SA Brown EE Petri MA Gallant C Ramsey-Goldman R Reveille JD Vila LM Criswell LA Edberg JC Freedman BI Gregersen PK Gilkeson GS Jacob CO James JA Kamen DL Kimberly RP Martin J Merrill JT Niewold TB Park SY Pons-Estel BA Scofield RH Stevens AM Tsao BP Vyse TJ Langefeld CD Harley JB Moser KL Webb CF Humphrey MB 《Nature genetics》2011,43(3):253-258
Systemic lupus erythematosus (SLE, MIM152700) is an autoimmune disease characterized by self-reactive antibodies resulting in systemic inflammation and organ failure. TNFAIP3, encoding the ubiquitin-modifying enzyme A20, is an established susceptibility locus for SLE. By fine mapping and genomic re-sequencing in ethnically diverse populations, we fully characterized the TNFAIP3 risk haplotype and identified a TT>A polymorphic dinucleotide (deletion T followed by a T to A transversion) associated with SLE in subjects of European (P = 1.58 × 10(-8), odds ratio = 1.70) and Korean (P = 8.33 × 10(-10), odds ratio = 2.54) ancestry. This variant, located in a region of high conservation and regulatory potential, bound a nuclear protein complex composed of NF-κB subunits with reduced avidity. Further, compared with the non-risk haplotype, the haplotype carrying this variant resulted in reduced TNFAIP3 mRNA and A20 protein expression. These results establish this TT>A variant as the most likely functional polymorphism responsible for the association between TNFAIP3 and SLE. 相似文献
49.
Digital city, a large complex system, is comprised many subsystem of different fields. It involves common field data, flexible field processes, seamless intra-organization and inter-organization collaborations, and increasing connectivity and interoperability between distributed or decentralized systems. All above these are involved Foundation Data of Digital city. To promote the sustainable development of Digital City, this paper investigates to the construction of Foundation Data Platform {FDP) from the view of the architecture. The paper introduces the concep- tions, characteristics and underlying problems of the FDP, and discusses the key issues of the architecture paradigms what FDP might adopted via comparing Object-Oriented Architecture {OOA), Component-Based Architecture (CBA) , and Service-based Architecture (SBA). The Process Embedded Service-Oriented Architecture (SOA), one of the recent SOA Application Architecture is introduced. The results indicate that the Process Embedded SOA could promote the sustainable development of Digital City and accommodate the dynamic development environments of different fields which constantly changes requirements. 相似文献
50.
With the development of location technologies, advanced LBSbased ITS increasingly requires the capability of database technologies to manage the continuously arrived vehicles' location, traffic jam and other interrelated information of large amounts of traffic in the following years. And some burst arrival stream data will challenge the realtime performance and the allocation of limited resource. However, choosing a desirable database operator scheduling strategy can significantly improve the performance of the system. The path capability strategy was chosen and improved as ITS' operator scheduling strategy to meet the realtime response and the minimal memory requirement of the system.
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