Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae

The aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. Here we report the genome sequence of the model organism Aspergillus nidulans, and a comparative study with Aspergillus fumigatus, a serious human pathogen, and Aspergillus oryzae, used in the production of sake, miso and soy sauce. Our analysis of genome structure provided a quantitative evaluation of forces driving long-term eukaryotic genome evolution. It also led to an experimentally validated model of mating-type locus evolution, suggesting the potential for sexual reproduction in A. fumigatus and A. oryzae. Our analysis of sequence conservation revealed over 5,000 non-coding regions actively conserved across all three species. Within these regions, we identified potential functional elements including a previously uncharacterized TPP riboswitch and motifs suggesting regulation in filamentous fungi by Puf family genes. We further obtained comparative and experimental evidence indicating widespread translational regulation by upstream open reading frames. These results enhance our understanding of these widely studied fungi as well as provide new insight into eukaryotic genome evolution and gene regulation.     

The human chorionic gonadotrophin bio-assay by seminal vesicles in mice

Zusammenfassung Biologische Methode zur Bestimmung von Choriogonadotrophin (HCG) im Harn, welche auf Gewichtszunahme von Samenblasen juveniler Mäuse basiert.     

Relationship between the chemical structure of thiazolidine acetic acid derivatives and the antiherpetic activity in human embryonic cell cultures

Zusammenfassung Durch Abwandlung der Struktur der 4-Oxo-5-thiazolidinessigsäure ergeben sich starke inhibitorische Effekte auf die cytopathogenen Wirkungen, die vom VirusHerpes simplex in Kulturen menschlicher Embryonalzellen ausgeübt werden.     

Patient Safety Culture,Evidence-Based Practice and Performance in Nursing

Evidence-based practice is a problem-solving approach in which the best available and useful evidence is used by integrating research evidence, clinical expertise, and patient values and preferences to improve health outcomes, service quality, patient safety and clinical effectiveness, and employee performance. This study aimed to identify the effects of nurses’ patient safety culture perceptions and their evidence-based nursing attitudes on the hospital’s patient safety level and employee performance. A cross-sectional design was employed in this study. Participants included nurses working in a state hospital in Burdur, Turkey, and 218 nurses responded to the research instrument (participation rate: 55.7%). Data were collected through face to face interviews that were conducted from February 20–May 20, 2016. The results of the analyses revealed that evidence-based nursing attitudes and hospital safety culture dimensions explained 29.2% of the total variance in the hospital patient safety level, while evidence-based nursing attitudes and hospital safety culture dimensions explained 15.5% of the total variance in nurse performance. The “evidence-based nursing related beliefs and expectations” aspect of the evidence-based nursing attitude dimension, and the “management support for patient safety” aspect of the patient safety culture dimension were significant predictors of nurses’ performance. The present findings add to the increasing interest in improving evidence-based practice and service quality in order to achieve better patient outcomes.     

秦皇岛海水浴场海滩小型底栖动物丰度的研究

2013年5月、9月和10月,在秦皇岛东山浴场海滩对小型底栖动物进行采样,对其平均丰度、各潮带水平分布、垂直分布和优势类群进行了研究。结果显示:小型底栖动物的平均丰度为695.26 ind/10 cm~2,5月份最低为428.58 ind/10 cm~2,9月份为504.99 ind/10 cm~2,10月份最高达到1 152.20 ind/10 cm~2;低潮带平均丰度为261.68 ind/10 cm~2,中潮带为318.54 ind/10 cm~2,高潮带为115.04 ind/10 cm~2;主要小型底栖动物类群为自由生活的海洋线虫、桡足类、多毛类、枝角类、涡虫类和端足类等,其中,海洋线虫为第一绝对优势类群,平均占93.01%,多毛类和涡虫类分别为第二、三优势类群,平均分别占3.28%和2.81%;小型底栖动物垂直分布由表层向底层呈减少的趋势。此外,海洋线虫与桡足类的丰度比为695.69,大于100,初步判断为有污染。     

职业人文教育：逻辑解构与理论重构--兼与张少兰老师商榷

职业人文教育命题的提出有其现实必要性与可能性，应然的职业人文教育应为职业教育的题中应有之义，但由于其理论范式和实践路径缺乏顶层设计，因此必须重塑并彰显职业人文教育的本然要义。重构的职业人文教育是以人文精神为旨归，以培养“成人”为其价值追求的职业教育，它是一种存在教育、素质教育与生态教育。实践职业人文教育，必须从职业教育的顶层设计、课程设计和教学设计上入手，进行理论范式的转型与渗透。这是就张少兰老师对“职业人文教育”理论的生成语境、价值追求和实践路径选取等命题的质疑的理论回应。     

对映选择性Diels-Alder反应中的化学酶

对近些年来发展起来的催化对映选择性ＤｉｅｌｓＡｌｄｅｒ反应的化学酶进行了综述     

四变量复合项组合险象精确定位

根据逻辑余式的原理，利用k=1状态变化过程对，在二变量组合险象的基础上，运用快速简化计算方法，精确定位四变量输入逻辑函数组合险象204163项，为多输入无组合险象数字电路的设计奠定了强有力的基础。     

基于对象感知的城市物流服务需求调研方法

为了有效获取真实有用的物流服务决策信息,确实提高城市物流需求预测的精度、效度,文中采取感知研究、举例研究、实证研究等方法,在分析物流服务需求的特征及传统调研方法的无效性的基础上,进行了服务需求调研方法的要素构建与过程剖析,并提出了基于对象感知的物流服务需求凋研方法体系,最终阐明了合适的感知对象和有效的感知方法对获取城市物流服务真实需求的重要性.