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921.
K. Kang C. Y. Kan Y. DU D. S. Liu 《复旦学报(自然科学版)》2005,44(5):738-739
In the past decades, more and more studies have been focused on the synthesis of monodisperse particles with different diameter by special polymerization technique. In 1980' s, Ugelstad, et al invented two-step swelling method to prepare monodisperse microsphere with large size more than 1μm. In the following decade, Okubo and his coworkers synthesized monodisperse crosslinked polymer particles above 3μm using one-step dynamic swelling method. New method has been developed to produce particles more than 50 gm in diameter with a standard deviation of less than 2 % . Up to now, most of the monodisperse particles were usually prepared by polymerization of St in the presence of surfactants. In this presentation, sub-micro sized P (MMA-EA-AA/MAA) particles with narrow size distribution were prepared by seeded emulsion polymerization in the absence of any surfactant materials. 相似文献
922.
Y. Kumagai M. Shinyashiki G. F. Sun N. Shimojo M. Sagai 《Cellular and molecular life sciences : CMLS》1994,50(7):673-676
Cuprozinc superoxide dismutase (Cu,Zn-SOD) was isolated from bovine erythrocytes by pH-controlled ammonium sulfate-methanol extraction (ASME extraction). Adjustment of the pH of a suspension of the lysed red cells in the presence of ammonium sulfate (90% saturation) to pH 5.0, followed by partition with an equal amount of methanol, resulted in isolation of the enzyme with specific, activity of greater than 2000 units/mg of protein. Further purification using DEAE-cellulose column chromatography gave a highly purified Cu,Zn-SOD showing a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Using this procedure about 14 mg of pure Cu,Zn-SOD with a specific activity of 4728 units/mg of protein can be recovered from one liter of bovine blood. The enzyme was characterized and the results obtained were in agreement with earlier reports. This procedure appears, therefore, to be a convenient method for isolating the enzyme. 相似文献
923.
J. M. Carlin Y. Ozaki G. I. Byrne R. R. Brown E. C. Borden 《Cellular and molecular life sciences : CMLS》1989,45(6):535-541
Summary Indoleamine 2,3-dioxygenase (IDO) is an interferon (IFN)-induced protein that initiates the metabolism of tryptophan along the kynurenine pathway. Although IDO can be induced by IFN- in many cell types, only mononuclear phagocytes have been shown to be induced to decyclize tryptophan by all three IFN classes. Since tryptophan is an essential amino acid necessary for a variety of metabolic processes, depletion of available tryptophan may be an important mechanism for control of rapidly-dividing microbial pathogens and tumors. The purpose of this review is to present evidence that documents the effects of IFN-induced IDO on prokaryotic and eukaryotic pathogens, as well as on a variety of tumor cell lines. 相似文献
924.
H. Minakata H. Komura S. Y. Tamura Y. Ohfune K. Nakanishi T. Kada 《Cellular and molecular life sciences : CMLS》1985,41(12):1622-1623
Summary Five unusual amino acids were identified as antimutagens against spontaneous mutation ofSalmonella typhimurium TA100: L-azetidine-2-carboxylic acid (1) from Liliaceae plants, -(methylenecyclopropyl)glycine (2) fromLitchi chinensis seeds, and 2-amino-4-methylhex-5-ynoic acid (3), hypoglycin A (4), and (2S, 4R)-2-amino-4-hydroxyhept-6-ynoic acid (5) fromEuphoria longana seeds. The absolute stereochemistry of5 was determined by its chiral synthesis from L-allylglycine, proving that5 is the C-4 epimer of the amino acid previously isolated from dried longan seeds.This work was partly supported by a Grant-in-Aid form the Ministry of Education, Science, and Culture, Japan, No. 57740283 (to HK). 相似文献
925.
Summary A new N-pyridinyl benzamide was found to potentiate strongly the effects of apomorphine on the motility of reserpinized mice and on circling behavior. Since dopaminergic agonist activity could not account for this potentiation, involvement of-adrenergic agonist activity provided the only consistent explanation.Acknowledgments. We wish to thank Mr A. Decoodt for his excellent technical assistance and the Laboratoire de Chimie Therapeutique (Prof. Le Baut), Université de Nantes, for providing samples of N-PCB. 相似文献
926.
927.
生物膜是由降解底物的活性物质与非活性物质所组成.基于这种观点,提出了系统描述生物膜中活性生物量(Ma)与非活性物质量(Mi)增长的生物数学模型体系.从理论上阐明了活性生物量与非活性物质量间动态的相互作用,并论证了生物膜反应器在运行中存在最大活性生物量(Ma)max,且在反应器达到最大活性生物量(Ma)max以后,非活性物质量的积累导致生物膜的继续增长,但不能改善生物膜反应器的出水物质. 相似文献
928.
929.
In crustaceans, all the steps in the assimilation of food take place in the hepatopancreas. To facilitate the study of this organ, a method for the dissociation of cell types was developed. The hepatopancreas of the prawn Palaemon serratus was mechanically dissociated and the cells separated by Percoll density-gradient centrifugation. The E and R cells had similar densities of around 1.05 g/ml. The F cells were separated into two distinct fractions with densities of 1.075 and 1.082 g/ml. The B cells sedimented at a density of 1.12 g/ml. The ratio between the two populations of F cells was found to vary during the intermolt cycle while B cells disappeared after the molt. When the density gradient fractions were incubated with 3H-leucine, incorporation was highest in the F cell fractions. Measurements of alpha-amylase activity, indicated that the two populations of F cells may be derived from the same cell type. 相似文献
930.